GPR97 (ADGRG3) (NM_170776) Human Untagged Clone

SKU SC100902

GPR97 (untagged)-ORIGENE UNIQUE VARIANT 1 of Human G protein-coupled receptor 97 (GPR97)

Product Data

• Type: Human Untagged Clone
• Target Symbol: GPR97
• Synonyms: GPR97; PB99; PGR26
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_170776, the custom clone sequence may differ by one or more nucleotides; ; ; ATGGCGACGCCCAGGGGCCTGGGGGCCCTGCTCCTGCTCCTCCTGCTCCCGACCTCAGGTCAGGAAAAGC; CCACCGAAGGGCCAAGAAACACCTGCCTGGGGAGCAACAACATGTACGACATCTTCAACTTGAATGACAA; GGCTTTGTGCTTCACCAAGTGCAGGCAGTCGGGCAGCGACTCCTGCAATGTGGAAAACTTGCAGAGATAC; TGGCTAAACTACGAGGCCCATCTGATGAAGGAAGGTTTGACGCAGAAGGTGAACACGCCTTTCCTGAAGG; CTTTGGTCCAGAACCTCAGCACCAACACTGCAGAAGACTTCTATTTCTCTCTGGAGCCCTCTCAGGTTCC; GAGGCAGGTGATGAAGGACGAGGACAAGCCCCCTGACAGAGTGCGACTTCCCAAGAGCCTTTTTCGATCC; CTGCCAGGCAACAGGTCTGTGGTCCGCTTGGCCGTCACCATTCTGGACATTGGTCCAGGGACTCTCTTCA; AGGGCCCCCGGCTCGGCCTGGGAGATGGCAGCGGCGTGTTGAACAATCGCCTGGTGGGTTTGAGTGTGGG; ACAAATGCATGTCACCAAGCTGGCTGAGCCTCTGGAGATCGTCTTCTCTCACCAGCGACCGCCCCCTAAC; ATGACCCTCACCTGTGTATTCTGGGATGTGACTAAAGGGACCACTGGAGACTGGTCTTCTGAGGGCTGCT; CCACGGAGGTCAGACCTGAGGGGACCGTGTGCTGCTGTGACCACCTGACCTTTTTCGCCCTGCTCCTGAG; ACCCACCTTGGACCAGTCCACGGTGCATATCCTCACACGCATCTCCCAGGCGGGCTGTGGGGTCTCCATG; ATCTTCCTGGCCTTCACCATTATTCTTTATGCCTTTCTGAGGCTTTCCCGGGAGAGGTTCAAGTCAGAAG; ATGCCCCAAAGATCCACGTGGCCCTGGGTGGCAGCCTGTTCCTCCTGAATCTGGCCTTCTTGGTCAATGT; GGGGAGTGGCTCAAAGGGGTCTGATGCTGCCTGCTGGGCCCGGGGGGCTGTCTTCCACTACTTCCTGCTC; TGTGCCTTCACCTGGATGGGCCTTGAAGCCTTCCACCTCTACCTGCTCGCTGTCAGGGTCTTCAACACCT; ACTTCGGGCACTACTTCCTGAAGCTGAGCCTGGTGGGCTGGGGCCTGCCCGCCCTGATGGTCATCGGCAC; TGGGAGTGCCAACAGCTACGGCCTCTACACCATCCGTGATAGGGAGAACCGCACCTCTCTGGAGCTATGC; TGGTTCCGTGAAGGGACAACCATGTACGCCCTCTATATCACCGTCCACGGCTACTTCCTCATCACCTTCC; TCTTTGGCATGGTGGTCCTGGCCCTGGTGGTCTGGAAGATCTTCACCCTGTCCCGTGCTACAGCGGTCAA; GGAGCGGGGGAAGAACCGGAAGAAGGTGCTCACCCTGCTGGGCCTCTCGAGCCTGGTGGGTGTGACATGG; GGGTTGGCCATCTTCACCCCGTTGGGCCTCTCCACCGTCTACATCTTTGCACTTTTCAACTCCTTGCAAG; GTGTCTTCATCTGCTGCTGGTTCACCATCCTTTACCTCCCAAGTCAGAGCACCACAGTCTCCTCCTCTAC; TGCAAGATTGGACCAGGCCCACTCCGCATCTCAAGAATAG; ;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_170776 unedited; NGACGTTCGTTTTTGTATACGACTCACTATAGGCGGCCGCGCTGGCGTGGGCAAGGCTGG; CCAAGGATGGCGACGCCCAGGGCCTGGGGGCCCTGCTCCTGCTCCTCCTGCTCCCGACCT; CAGGTCAGGAAAAGCCCACCGAAGGGCCAAGAAACACCTGCCTGGGGAGCAACAACATGT; ACGACATCTTCAACTTGAATGACAAGGCTTTGTGCTTCACCAAGTGCAGGCAGTCGGGCA; GCGACTCCTGCAATGTGGAAAACTTGCAGAGGTTCCGAGGCAGGTGATGAAGGACGAGGA; CAAGCCCCCTGACAGAGTGCGACTTCCCAAGAGCCTTTTTCGATCCCTGCCAGGCAACAG; GTCTGTGGTCCGCTTGGCCGTCACCATTCTGGACATTGGTCCAGGGACTCTCTTCAAGGT; GAGGACTCAGGGAAGCTCCAAGGTTAAGTGCTAGGTCCTCTGGGGGCTCCATGCCACAGT; TTGCTGTCACTGGAGCCTGCCGAGGGATCCAATGGGGACAGAGCAGGCAGTGAGCCTCTT; AGTGTTTCTAATGCAACCCGTGGCCATCTCATACACCTGTCACTAGAGTCTATGGTCTTC; AGACTCACACTGGTCACACGCTCTCATATGTTTGACCCCCACCCATGACGGCCCCTTTGG; GCTTGGCTTATGTGACCTTGTGGATGCTCCGTCTTCTTTCTAGTGTAACTCTTTTTTAAA; AAAAGGCGGACACATGCCTACGCGCACGTCGTCGGACCCCGCTAATACTCGCCAACCCCG; ACACTGGAATAATATACTGCGCGTCACCCACATCTGCCCCAGTATCCATCCCCATTATTT; TATTCCACGCCGCCGGTAAATCTTACCCCCCCTACCTACCATCCGCGGAATCGAACGTTC; TTGTCATGCGCATAAAAATATACGCGCCCT;
• Restriction Sites: NotI-NotI
• ACCN: NM_170776
• Insert Size: 3000 bp
• OTI Disclaimer: The sequence of an 'OriGene Unique Variant' differs significantly from the associated reference. It represents a novel splice variant from the same gene locus of the reference. Although such variants are true transcripts and present opportunity for discoveries, they are not yet curated by NCBI and should not be used if the exact reference accession sequence is required.
• OTI Annotation: This TrueClone was found to represent an alternative form of the specific reference to which it is associated. Its Open Reading Frame (ORF) may represent a novel form or alternative splice variant. By virtue of it being a true transcript (cDNA clone not PCR product), it provides a biologically relevant copy of its mRNA template. For more details, please evaluate the sequence information provided on this website or contact our customer care specialists.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_170776.2, NP_740746.2
• RefSeq Size: 2676 bp
• RefSeq ORF: 2676 bp
• Locus ID: 222487
• UniProt ID: Q86Y34
• Cytogenetics: 16q21
• Protein Families: Druggable Genome, GPCR, Transmembrane
• Summary: Orphan receptor that regulates migration of lymphatic endothelial cells in vitro via the small GTPases RhoA and CDC42 (PubMed:24178298). Regulates B-cell development (By similarity). Seems to signal through G-alpha(q)-proteins (PubMed:22575658).[UniProtKB/Swiss-Prot Function]; Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).