Repulsive Guidance Molecule A (RGMA) (NM_020211) Human Untagged Clone

SKU SC100678

RGMA (untagged)-Human RGM domain family, member A (RGMA), transcript variant 4

Product Data

• Type: Human Untagged Clone
• Target Symbol: Repulsive Guidance Molecule A
• Synonyms: RGM
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC100678 sequence for NM_020211 edited (data generated by NextGen Sequencing); ATGCAGCCGCCAAGGGAGAGGCTAGTGGTAACAGGCCGAGCTGGATGGATGGGTATGGGG; AGAGGGGCAGGACGTTCAGCCCTGGGATTCTGGCCGACCCTCGCCTTCCTTCTCTGCAGC; TTCCCCGCAGCCACCTCCCCGTGCAAGATCCTCAAGTGCAACTCTGAGTTCTGGAGCGCC; ACGTCGGGCAGCCACGCCCCAGCCTCAGACGACACCCCCGAGTTCTGTGCAGCCTTGCGC; AGCTACGCCCTGTGCACGCGGCGGACGGCCCGCACCTGCCGGGGTGACCTGGCCTACCAC; TCGGCCGTCCATGGCATAGAGGACCTCATGAGCCAGCACAACTGCTCCAAGGATGGCCCC; ACCTCGCAGCCACGCCTGCGCACGCTCCCACCGGCCGGAGACAGCCAGGAGCGCTCGGAC; AGCCCCGAGATCTGCCATTACGAGAAGAGCTTTCACAAGCACTCGGCCACCCCCAACTAC; ACGCACTGTGGCCTCTTCGGGGACCCACACCTCAGGACTTTCACCGACCGCTTCCAGACC; TGCAAGGTGCAGGGCGCCTGGCCGCTCATCGACAATAATTACCTGAACGTGCAGGTCACC; AACACGCCTGTGCTGCCCGGCTCAGCGGCCACTGCCACCAGCAAGCTCACCATCATCTTC; AAGAACTTCCAGGAGTGTGTGGACCAGAAGGTGTACCAGGCTGAGATGGACGAGCTCCCG; GCCGCCTTCGTGGATGGCTCTAAGAACGGTGGGGACAAGCACGGGGCCAACAGCCTGAAG; ATCACTGAGAAGGTGTCAGGCCAGCACGTGGAGATCCAGGCCAAGTACATCGGCACCACC; ATCGTGGTGCGCCAGGTGGGCCGCTACCTGACCTTTGCCGTCCGCATGCCAGAGGAAGTG; GTCAATGCTGTGGAGGACTGGGACAGCCAGGGTCTCTACCTCTGCCTGCGGGGCTGCCCC; CTCAACCAGCAGATCGACTTCCAGGCCTTCCACACCAATGCTGAGGGCACCGGTGCCCGC; AGGCTGGCAGCCGCCAGCCCTGCACCCACAGCCCCCGAGACCTTCCCATACGAGACAGCC; GTGGCCAAGTGCAAGGAGAAGCTGCCGGTGGAGGACCTGTACTACCAGGCCTGCGTCTTC; GACCTCCTCACCACGGGCGACGTGAACTTCACACTGGCCGCCTACTACGCGTTGGAGGAT; GTCAAGATGCTCCACTCCAACAAAGACAAACTGCACCTGTATGAGAGGACTCGGGACCTG; CCAGGCAGGGCGGCTGCGGGGCTGCCCCTGGCCCCCCGGCCCCTCCTGGGCGCCCTGGTC; CCGCTCCTGGCCCTGCTCCCTGTGTTCTGCTAG; ; Clone variation with respect to NM_020211.2; 1317 c=>g
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_020211 unedited; ATAGGGCGCGCCGCGAATTCGCACGAGGGACGAGCGGCGCCCCGACACCCCCTCTTCTCC; CGCAGCCCCGCCAGCGCCACCCCCCGCGGGCCGCAGGGGCTCATGCAGCCGCCAAGGGAG; AGGCTAGTGGTAACAGGCCGAGCTGGATGGATGGGTATGGGGAGAGGGGCAGGACGTTCA; GCCCTGGGATTCTGGCCGACCCTCGCCTTCCTTCTCTGCAGCTTCCCCGCAGCCACCTCC; CCGTGCAAGATCCTCAAGTGCAACTCTGAGTTCTGGAGCGCCACGTCGGGCAGCCACGCC; CCAGCCTCAGACGACACCCCCGAGTTCTGTGCAGCCTTGCGCAGCTACGCCCTGTGCACG; CGGCGGACGGCCCGCACCTGCCGGGGTGACCTGGCCTACCACTCGGCCGTCCATGGCATA; GAGGACCTCATGAGCCAGCACAACTGCTCCAAGGATGGCCCCACCTCGCAGCCACGCCTG; CGCACGCTCCCACCGGCCGGAGACAGCCAGGAGCGCTCGGACAGCCCCGAGATCTGCCAT; TACGAGAAGAGCTTTCACAAGCACTCGGCCACCCCCAACTACACGCACTGTGGCCTCTTC; GGGGACCCACACCTCAGGACTTTCACCGACCGCTTCCAGACCTGCAAGGTGCANGGCGCC; TGGCCGCTCATCGNACATAATTACCTGAACGTGCAGGTCACCAACACGCCTGTGCTGCCC; GGCTCAGCGGNCACTGCCACCAGCAAGCTCACCATCATCTGCAAGAACTTCANGAGTGTG; TGGACCANAAGGTGTACCANGCTGAGATGGACGAGCTNCCNGNCGNCTTCGTGGATGGCT; CTANNNAACGTGGGGACATGCACGGGGCCACAGCCTGNAGATCACTGAGAAGGGGGTCAG; CCACACTTGGAGATCCAGNCCAGTACTTNNGACACCATA;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_020211 unedited; GCTTGGACGCGGCCGTTATNCTANAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT; TTTTTTTTTTTTTTTAAAAGAACAAAAAAAACATATTTTATTTGTTTTTACCCATAAATA; AAAAATTTAAAGTAGGTACACGCGGGGAAATTTTAAACAGGTGGAAAACAAGTGCACGCA; CGGGGGGGTCCCCCCTTCCAACCAGAATGGGAGCTACCACATACAAAATAAAGGGCTTCA; TTTTTAATTTCCACCATAGGGACCAACCAAAACCAACAGGGAAAGGGAGGGGCCGGCCCA; CAAACGCTCTCCACATTCTATACACACCGTTTTGCTAGAGGGACCACCACTTTTTGGAGG; GAGGCGGAAACTCACGTTCCCCCCCCCCTTGACCCGCGATTAACAGGTTCTTGGGTAACA; CCACAGGCCCTCCTATAGGGCCGGCCAATGGTGTGCACCTCCCTCTTCCCGCTTCCCCCA; CCCCCCCGTTCACCCTCCTTCCCCTATCCCAACCCCTCTTCGCCTTACTCCCATCATTCC; CGCGTCTCCCGTTCCCCCTTCTTTCCTCCCCCTCTTTTCCTTTATGGCCCCCCCAATTTC; CTCATCCCCCCATTCTTATCCTCTTCCTACCCCCATCCCTCCTTCCCCCATTCTTTCCCC; CCCCCCCCATTTCTTCTATCTCCCTTCTCATCCCACTCCCTTTCCCTACTCCCTTTTCCC; CTATCTATTTTCCCCCCCCCTTTTTTTCCTTCCCGCTCTATCTTTCCTTTAATTATTCCA; ACTTGCCCCCATCCCTTTCCCCCCCCCTCCCGCTCACCTCCTATTATATCACTCTCCCAC; ACCTAATTCCACTCCCCCTCTTTCCACATTTACCGCATTCTCTCCGCCCCTAACAAC;
• Restriction Sites: NotI-NotI
• ACCN: NM_020211
• Insert Size: 3000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_020211.1, NP_064596.1
• RefSeq Size: 3216 bp
• RefSeq ORF: 1353 bp
• Locus ID: 56963
• UniProt ID: Q96B86
• Cytogenetics: 15q26.1
• Summary: This gene encodes a member of the repulsive guidance molecule family. The encoded protein is a glycosylphosphatidylinositol-anchored glycoprotein that functions as an axon guidance protein in the developing and adult central nervous system. This protein may also function as a tumor suppressor in some cancers. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Oct 2009]; Transcript Variant: This variant (4) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at an alternate start codon, compared to variant 1. The encoded isoform (3) has a distinct N-terminus and is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.