Nicastrin (NCSTN) (NM_015331) Human Untagged Clone

SKU SC100655

NCSTN (untagged)-Human nicastrin (NCSTN)

Product Data

• Type: Human Untagged Clone
• Target Symbol: Nicastrin
• Synonyms: ATAG1874
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC100655 sequence for NM_015331 edited (data generated by NextGen Sequencing); ATGGCTACGGCAGGGGGTGGCTCTGGGGCTGACCCGGGAAGTCGGGGTCTCCTTCGCCTT; CTGTCTTTCTGCGTCCTACTAGCAGGTTTGTGCAGGGGAAACTCAGTGGAGAGGAAGATA; TATATCCCCTTAAATAAAACAGCTCCCTGTGTTCGCCTGCTCAACGCCACTCATCAGATT; GGCTGCCAGTCTTCAATTAGTGGAGACACAGGGGTTATCCACGTAGTAGAGAAAGAGGAG; GACCTACAGTGGGTATTGACTGATGGCCCCAACCCCCCTTACATGGTTCTGCTGGAGAGC; AAGCATTTTACCAGGGATTTAATGGAGAAGCTGAAAGGGAGAACCAGCCGAATTGCTGGT; CTTGCAGTGTCCTTGACCAAGCCCAGTCCTGCCTCAGGCTTCTCTCCTAGTGTACAGTGC; CCAAATGATGGGTTTGGTGTTTACTCCAATTCCTATGGGCCAGAGTTTGCTCACTGCAGA; GAAATACAGTGGAATTCGCTGGGCAATGGTTTGGCTTATGAAGACTTTAGTTTCCCCATC; TTTCTTCTTGAAGATGAAAATGAAACCAAAGTCATCAAGCAGTGCTATCAAGATCACAAC; CTGAGTCAGAATGGCTCAGCACCAACCTTCCCACTATGTGCCATGCAGCTCTTTTCACAC; ATGCATGCTGTCATCAGCACTGCCACCTGCATGCGGCGCAGCTCCATCCAAAGCACCTTC; AGCATCAACCCAGAAATCGTCTGTGACCCCCTGTCTGATTACAATGTGTGGAGCATGCTA; AAGCCTATAAATACAACTGGGACATTAAAGCCTGACGACAGGGTTGTGGTTGCTGCCACC; CGGCTGGATAGTCGTTCCTTTTTCTGGAATGTGGCCCCAGGGGCTGAAAGCGCAGTGGCT; TCCTTTGTCACCCAGCTGGCTGCTGCTGAAGCTTTGCAAAAGGCACCTGATGTGACCACC; CTGCCCCGCAATGTCATGTTTGTCTTCTTTCAAGGGGAAACTTTTGACTACATTGGCAGC; TCGAGGATGGTCTACGATATGGAGAAGGGCAAGTTTCCCGTGCAGTTAGAGAATGTTGAC; TCATTTGTGGAGCTGGGACAGGTGGCCTTAAGAACTTCATTAGAGCTTTGGATGCACACA; GATCCTGTTTCTCAGAAAAATGAGTCTGTACGGAACCAGGTGGAGGATCTCCTGGCCACA; TTGGAGAAGAGTGGTGCTGGTGTCCCTGCTGTCATCCTCAGGAGGCCAAATCAGTCCCAG; CCTCTCCCACCATCTTCCCTGCAGCGATTTCTTCGAGCTCGAAACATCTCTGGCGTTGTT; CTGGCTGACCACTCTGGTGCCTTCCATAACAAATATTACCAGAGTATTTACGACACTGCT; GAGAACATTAATGTGAGCTATCCCGAATGGCTGAGCCCTGAAGAGGACCTGAACTTTGTA; ACAGACACTGCCAAGGCCCTGGCAGATGTGGCCACGGTGCTGGGACGTGCTCTGTATGAG; CTTGCAGGAGGAACCAACTTCAGCGACACAGTTCAGGCTGATCCCCAAACGGTTACCCGC; CTGCTCTATGGGTTCCTGATTAAAGCCAACAACTCATGGTTCCAGTCTATCCTCAGGCAG; GACCTAAGGTCCTACTTGGGTGACGGGCCTCTTCAACATTACATCGCTGTCTCCAGCCCC; ACCAACACCACTTATGTTGTACAGTATGCCTTGGCAAATTTGACTGGCACAGTGGTCAAC; CTCACCCGAGAGCAGTGCCAGGATCCAAGTAAAGTCCCAAGTGAAAACAAGGATCTGTAT; GAGTACTCATGGGTCCAGGGCCCTTTGCATTCTAATGAGACGGACCGACTCCCCCGGTGT; GTGCGTTCTACTGCACGATTAGCCAGGGCCTTGTCTCCTGCCTTTGAACTGAGTCAGTGG; AGCTCTACTGAATACTCTACATGGACTGAGAGCCGCTGGAAAGATATCCGTGCCCGGATA; TTTCTCATCGCCAGCAAAGAGCTTGAGTTGATCACCCTGACAGTGGGCTTCGGCATCCTC; ATCTTCTCCCTCATCGTCACCTACTGCATCAATGCCAAAGCTGATGTCCTTTTCATTGCT; CCCCGGGAGCCAGGAGCTGTGTCATACTGA; ; Clone variation with respect to NM_015331.2;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_015331 unedited; GATTTTGTAATACGACTTACTATAGGGCGGCCGCGAATTCGGCACCGCGCTAACAGACAG; AGCCGAACGGGGGCTTCCGCTCAGCAGAGAGGCAAGATGGCTACGGCAGGGGGTGGCTCT; GGGGCTGACCCGGGAAGTCGGGGTCTCCTTCGCCTTCTGTCTTTCTGCGTCCTACTAGCA; GGTTTGTGCAGGGGAAACTCAGTGGAGAGGAAGATATATATCCCCTTAAATAAAACAGCT; CCCTGTGTTCGCCTGCTCAACGCCACTCATCAGATTGGCTGCCAGTCTTCAATTAGTGGA; GACACAGGGGTTATCCACGTAGTAGAGAAAGAGGAGGACCTACAGTGGGTATTGACTGAT; GGCCCCAACCCCCCTTACATGGTTCTGCTGGAGAGCAAGCATTTTACCAGGGATTTAATG; GAGAAGCTGAAAGGGAGAACCAGCCGAATTGCTGGTCTTGCAGTGTCCTTGACCAAGCCC; AGTCCTGCCTCAGGCTTCTCTCCTAGTGTACAGTGCCCAAATGATGGGTTTGGTGTTTAC; TCCAATTCCTATGGGCCAGAGTTTGCTCACTGCAGAGAAATACAGTGGAATTCGCTGGGC; AATGGTTTGGCTTATGAAGACTTTAGTTTCCCCATCTTTCTTCTTGAAGATGAAAATGAA; ACCAAAGTCATCAAGCAGTGCTATCAAGATCACAACCTGAGTCAGAATGGCTCAGCACCA; ACCTTCCCACTATGTGCCATGCAGCTCTTTTCACACATGCATGCTGTCATCAGCACTGCC; ACCTGCATGCNGCGCAGCTCCATCCAAAGCACCTTCAGCATCAACCCAGAAATCGTCTGT; GACCCCCTGTCTGATTACCATGTGTGGAGCATGCTAAAGCCTATNAATACCACTGGGACA; TTAAAGCCTGACGACAGGGTTGTGGNTTGCTGCACCNNGCTGATAAGTCGTCCTTTTTCT; GGATGTGGCCCCAGGGGCTGAAGCGCATGGCTTCTTTGTACCCACTGGCG;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_015331 unedited; GGGGGGTGAAAGTCAATGCTTTTTGATGNCAACTCTTAGTATATATTTACAAAAGCAATA; AGGAAAAAGACAAAAACAAAAATAGATATTACAGCTTAATAAAAAGTACAACTGAGCACT; GTGGGCTGGAGGTGGGATACCCACCCGCAGCACGCCCACCCTAATGTGCCATCTGAGGGC; CTGCCACAAAGGACAGTGGGCAACGAGACAGTAGGCTCAGCCCATATATTCCTCTGTCCT; CAGGGGCTCAATGTACCTTGACCCTGACATTAAACCTTTTATGTACCTTCCCAGTCACGA; AGGTGGCTTGGGTACATTGGATGCGGGCGCGCGCGATCATTATAGAGGTACAGGGCAGGA; AGGAAAAAGTAGAAGGGTCCTGAAGGGCCAATTATGGTGGGTAACTAGGGAGTTAAACAG; GAGCAAGAAGCTTCTATTTGTAATCCCGCCCTCTGGCATGACGCCGAGAAGGAAGAGGAA; ATGGGGAAGGGGTGATGGGAAAGGGGAGCGGATTGAAGTATAATCGGTGACTTTCTCTAC; ACCGATTTTCGGAATTTGCCATATTTTTTTTCCCCCGCTATTTTTTCCGCCCCCCTCCAT; CCCCCGAAATTAATGTTATGGGTTTTCACTTTACGGCGCCCACCTCTCCATTCATCCCCC; CTGATTGTTTCTCCCCATGAGCCCCCCTTTTGTTTGGCGCCCAATTGGACCAGCGGTATT; AGTTCCCACTGATTTGTATTTGTGCATAAAACGAGAGGATTGCGCCGTTTTTATGTTGTC; CCGACTGTTGGGTCCTGCCTGACTCTAATCGGCTGTAATGGGCCGTATAGAATTACGTTT; AGGCCCGGTCGCCTGTTTTTTCTCCCGTTTGTGTCGCCCCCCCCCCCCCGTTTTTTGTTT; TATTTN;
• Restriction Sites: Please inquire
• ACCN: NM_015331
• Insert Size: 3000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_015331.2, NP_056146.1
• RefSeq Size: 2944 bp
• RefSeq ORF: 2130 bp
• Locus ID: 23385
• UniProt ID: Q92542
• Cytogenetics: 1q23.2
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Alzheimer's disease, Notch signaling pathway
• Summary: This gene encodes a type I transmembrane glycoprotein that is an integral component of the multimeric gamma-secretase complex. The encoded protein cleaves integral membrane proteins, including Notch receptors and beta-amyloid precursor protein, and may be a stabilizing cofactor required for gamma-secretase complex assembly. The cleavage of beta-amyloid precursor protein yields amyloid beta peptide, the main component of the neuritic plaque and the hallmark lesion in the brains of patients with Alzheimer's disease; however, the nature of the encoded protein's role in Alzheimer's disease is not known for certain. Mutations in this gene are associated with familial acne inversa. A pseudogene of this gene is present on chromosome 21. Alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined. [provided by RefSeq, Feb 2014]; Transcript Variant: This variant (1) encodes the longest isoform (1).