Mitocondrial Translational Initiation Factor 3 (MTIF3) (NM_152912) Human Untagged Clone

SKU SC100648

MTIF3 (untagged)-Human mitochondrial translational initiation factor 3 (MTIF3), nuclear gene encoding mitochondrial protein, transcript variant 2

Product Data

• Type: Human Untagged Clone
• Target Symbol: Mitocondrial Translational Initiation Factor 3
• Synonyms: IF3mt
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_152912, the custom clone sequence may differ by one or more nucleotides; ; ; ATGGCTGCTCTTTTTCTAAAGAGGTTAACACTACAAACTGTAAAGTCTGAAAATAGTTGCATTAGATGTT; TTGGTAAACACATCCTGCAAAAGACAGCACCAGCACAGTTGTCCCCTATTGCTTCTGCCCCAAGACTCTC; CTTCCTAATTCATGCAAAAGCCTTTAGTACCGCTGAAGACACCCAGAATGAAGGAAAAAAGACAAAAAAG; AATAAAACAGCTTTTAGTAACGTTGGAAGAAAAATTAGTCAGCGAGTTATTCACTTATTTGATGAGAAGG; GCAATGATTTGGGAAACATGCACCGAGCAAATGTGATTAGACTTATGGATGAGCGAGACCTGCGACTGGT; TCAAAGGAACACCAGCACAGAACCTGCAGAGTATCAGCTCATGACAGGATTGCAGATCCTCCAGGAGCGG; CAGAGGCTGAGGGAGATGGAGAAGGCGAACCCCAAAACTGGACCAACCCTGAGAAAGGAACTGATTTTGT; CTTCAAATATTGGACAACATGATTTGGACACAAAGACTAAACAGATTCAGCAGTGGATTAAGAAAAAACA; CCTAGTCCAGATTACCATAAAGAAAGGAAAAAATGTAGACGTGTCAGAAAATGAAATGGAGGAGATATTT; CATCAAATACTCCAGACTATGCCTGGAATAGCTACATTCTCATCTAGGCCACAAGCTGTTCAAGGAGGAA; AAGCTTTAATGTGTGTTCTTCGTGCTTTCAGCAAAAATGAGGAGAAGGCATATAAAGAAACTCAAGAGAC; CCAGGAAAGAGACACTTTGAACAAAGACCATGGAAATGATAAGGAATCAAATGTTCTGCATCAGTAA; ;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_152912 unedited; AAAAANAAAAAAANAACANCCAACANNGGGCCCGGTCCAGCATTTGTCATACGACTCACT; ATAGGCGGCCGCGAATTCTTCCTAAAAAAGATGGCTGCTCTTTTTCTAAAGAGGTTAACA; CTACAAACTGTAAAGTCTGAAAATAGTTGCATTAGATGTTTTGGTAAACACATCCTGCAA; AAGACAGCACCAGCACAGTTGTCCCCTATTGCTTCTGCCCCAAGACTCTCCTTCCTAATT; CATGCAAAAGCCTTTAGTACCGCTGAAGACACCCAGAATGAAGGAAAAAAGACAAAAAAG; AATAAAACAGCTTTTAGTAACGTTGGAAGAAAAATTAGTCAGCGAGTTATTCACTTATTT; GATGAGAAGGGCAATGATTTGGGAAACATGCACCGAGCAAATGTGATTAGACTTATGGAT; GAGCGAGACCTGCGACTGGTTCAAAGGAACACCAGCACAGAACCTGCAGAGTATCAGCTC; ATGACAGGATTGCAGATCCTCCAGGAGCGGCAGAGGCTGAGGGAGATGGAGAAGGCGAAC; CCCANAACTGGACCAACCCTGAGAAAGGAACTGATTTTGTCTTCAAATATTGGACAACAT; GATTTGGACACAAAGACTAAACAGATTCAGCAGTGGATTAAGAAAAAACACCTAGTCCAG; ATTACCATAAAGAAAGGAAAAAATGTAGACGTGTCAGAAAATGAAATGGAGGAGATATTT; TATCAAATACTCCAGACTATGCCTGGAATACCTACATTCTCATCTAAGCCACAAGCTGTT; CAAGGGAGAAAAGCTTTTAATGTGTGTTCTTCGGGCTTTCCAACAAAATGAGGAGCAGGC; CTATTAAGAAACTCCAGAGACCCAGGAAAGAGACACTTTGAACAAAGAACCTGGGAATGA; TAAGGAATCAATGTTCTTGCTCAATCATTTAAATAAAGACAACCTGCTTCTGAAACCACC; AACNCTATCC;
• Restriction Sites: NotI-NotI
• ACCN: NM_152912
• Insert Size: 1100 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_152912.3, NP_690876.2
• RefSeq Size: 1018 bp
• RefSeq ORF: 837 bp
• Locus ID: 219402
• UniProt ID: Q9H2K0
• Cytogenetics: 13q12.2
• Domains: IF3_N
• Summary: This gene encodes a translation initiation factor that is involved in mitochondrial protein synthesis. Polymorphism in this gene is associated with the onset of Parkinson's disease. Alternate splicing results in multiple transcript variants. A pseudogene of this gene is found on chromosome 5. [provided by RefSeq, Oct 2009]; Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1, 2, 3, and 4 encode the same protein.