Coiled coil domain containing protein 111 (PRIMPOL) (NM_152683) Human Untagged Clone

SKU SC100629

CCDC111 (untagged)-Human coiled-coil domain containing 111 (CCDC111)

Product Data

• Type: Human Untagged Clone
• Target Symbol: Coiled coil domain containing protein 111
• Synonyms: CCDC111; MYP22; Primpol1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_152683, the custom clone sequence may differ by one or more nucleotides; ; ; ATGAATAGAAAATGGGAAGCAAAACTGAAGCAAATTGAAGAACGAGCATCTCATTATGAGAGGAAACCGT; TGTCCTCAGTGTATAGACCAAGATTGTCCAAGCCAGAAGAACCACCCTCCATCTGGAGACTATTTCATCG; ACAAGCTCAAGCTTTTAATTTTGTTAAAAGCTGTAAAGAAGACGTTCATGTATTTGCTTTGGAATGCAAA; GTAGGAGATGGACAACGTATTTACCTTGTGACAACCTATGCTGAATTTTGGTTTTACTATAAATCCAGAA; AAAATCTCTTACACTGCTATGAAGTTATTCCTGAAAATGCTGTGTGCAAGCTTTATTTTGATTTGGAATT; TAACAAACCTGCCAACCCAGGAGCTGATGGGAAAAAGATGGTTGCATTACTCATTGAGTATGTGTGTAAA; GCACTTCAAGAGTTATACGGTGTTAATTGCTCAGCTGAAGATGTTTTGAACTTGGATTCTAGCACTGATG; AAAAATTCAGCCGGCATTTAATATTTCAGCTCCATGATGTGGCATTTAAAGATAATATTCATGTTGGTAA; TTTTTTGAGAAAAATTTTGCAGCCTGCTCTTGACTTGCTTGGCAGTGAAGATGATGATAGCGCTCCAGAG; ACAACAGGCCATGGATTTCCCCATTTTTCAGAAGCACCTGCAAGACAAGGATTTTCTTTCAATAAAATGT; TCACAGAAAAGGCTACAGAGGAAAGCTGGACATCGAATTCAAAGAAACTGGAGAGGCTGGGGTCAGCTGA; GCAAAGCAGTCCTGACCTTTCATTTCTAGTTGTGAAGAATAACATGGGAGAGAAGCATCTTTTTGTAGAT; CTCGGAGTTTATACAAGAAATAGAAACTTTCGGCTATATAAATCATCAAAAATTGGAAAGCGTGTGGCTT; TGGAGGTTACTGAAGATAACAAATTTTTTCCTATACAGTCAAAAGATGTTTCTGACGAATATCAATATTT; TCTCTCTTCTTTGGTCAGCAATGTCAGGTTCTCAGATACTTTACGAATTCTTACATGTGAGCCATCTCAG; AATAAACAAAAAGGAGTTGGATATTTTAACAGTATCGGCACTTCAGTAGAAACCATTGAAGGTTTTCAGT; GTTCTCCCTATCCTGAAGTTGATCATTTTGTTCTTTCTTTGGTGAATAAAGATGGCATTAAAGGAGGAAT; TCGGCGTTGGAACTACTTTTTCCCAGAAGAATTACTGGTTTATGATATTTGTAAATATCGGTGGTGTGAA; AACATTGGAAGAGCCCATAAGAGTAATAATATAATGATTCTGGTTGATCTGAAAAATGAAGTTTGGTATC; AAAAATGTCATGACCCTGTATGTAAAGCAGAAAACTTCAAATCTGACTGTTTCCCATTACCTGCTGAAGT; ATGTCTCCTGTTTCTTTTCAAAGAGGAAGAAGAGTTTACAACAGATGAAGCAGATGAAACTAGGAGCAAT; GAAACCCAGAATCCTCATAAACCATCACCTAGCAGGCTGTCAACAGGTGCATCTGCTGATGCTGTCTGGG; ATAATGGCATTGATGATGCTTATTTTTTAGAAGCTACTGAAGATGCTGAATTAGCTGAAGCTGCAGAGAA; CAGTCTTCTCAGTTATAACAGTGAAGTGGATGAAATTCCTGATGAACTAATTATAGAAGTATTACAAGAG; TAA; ;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_152683 unedited; AGAATTTTGTAATACGACTCACTATAGGGCGGCCCGCGAATTCGCACGAGGCTCGCGTCC; CGCGCCAGTCCTCCCGCTTTGGTCCCGCCCTGAGGCTCGGCCCGGCTCCCCCGCCCCGGA; TTTGAAATCTAGGTCTTCCTGAGAGTCCGCGGGCTTCCGCTCCCAGCGCGGGATTCTGGA; GGGAAATTGAGGGAGACTTGGAAACCGCGCCAGGCCCAACGGGTACCTAGAAGGGAGACA; AAGCCAGGCCTGCTGCCCAGTTTGAGCTCTGGGAAGAAGAGGAGCAGGCCGGGACGCCCA; CCGAGGTATCCCAGAGGTGACAGAACTTGATCTGGGCCTGGAAGGGGAAGAATTCGGGAA; AAGCAACGTGGGGCATTCCAGTAGTAATTGATAGAAATATTACGTGGGATAGGATTTATT; CTCTCCACACTTCTGAACCAATGAATAGAAAATGGGAAGCAAAACTGAAGCAAATTGAAG; AACGAGCATCTCATTATGAGAGGAAACCGTTGTCCTCAGTGTATAGACCAAGATTGTCCA; AGCCAGAAGAACCACCCTCCATCTGGAGACTATTTCATCGACAAGCTCAAGCTTTTAATT; TTGTTAAAAGCTGTAAAGAAGACGTTCATGTATTTGCTTTGGAATGCAAAGTAGGAGATG; GACAACGTATTTACCTTGTGACAACCTATGCTGAATTNTGGTTTTACTATAAATCCAGAA; AAAATCTCTTACACTGCTATGAAGTTATTCCTGAAAATGCTGTGTGCAAGCTNTATTNTG; ATTTGGAATTTAACAAACCTGCCAACCCAGGAGCTGATGGNGAAAAGATGGTTGCATTAC; TCATTGAGTATGTGTGTAAAGCACTTNCAGAGTATACGGTGTTAATTGCTCAGCTGAGAT; GTNTGAACTTGGATCTACACTGATGAAAATCAGCCGCATTTATATTTCACTCATGATGTG; CATTAAGATATATCATGTGGTAATTNTTGAAA;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_152683 unedited; AATACTGTGNACCGCGGCCCGCTTTCTAGNGATCGGTTTTTTTTTTTTTTTTTTTTTTTT; TTTTTTTTTTTTTTTTTTTTACACCAAACCTAATTTTTAATGAAATTGGTTTACAGAAGT; AAAAAACAAAAATTGGAAAGCAAAGTAATAAAACTTAGTTTATATAAACAGGTTGAATGA; TATATTTATCAAATCTCACAGACATCAGGCAAATTATAGCCTGGGGACAAAAGTGTTCAT; AGGGAATTAGTTACCCTTGGAATACTTCTATAATTAGTTCATCAGGAATTTCATCCACTT; CACTGTTATAACTGAAAAAACTGTTCTCTGCAGCTTCAGCTAATTCAGCATCTTCAGTAG; CTTCTAAAAAATAAGCATCATCAATGCCATTATCCCAAACAGCATCAGCAGATGCCCCTT; TTGACAGCCTGCTAGGTGATGGTTTATGAGGATTCTGGGTTTCATTGCTCCTAGTTTCAT; CTGCTTCATCTGTTGGAAACTCTTCTTCCTCTTTGAAAAGAAACAGGAGACATACTTCAG; CAGGTAATGGGAAACAGTCAGATTTGAAGTTTTCTGCTTTACTACAGGGTCATGACATTT; TTGATACCAAACTTCATTTTTCAGATCAACCAGAATCATTATATTATTACTCTTATGGGC; TCTTCCAATGTTTTCCCACCACCGATATTTACAAATATCATAAACCAGTAATTCTTCTGG; GAAAAAAGTATTCCCAACGCCGAAATCCTCCTTTTAATGCATCTGAAGTGCCGAAACTGG; TTAAATATCCACTCCTTTTTGTTAATTCTGAGAGGCTCCCATGTAAAAATTCTAAAGATT; CGGAGACCCGGCTTTGTGACCAAGAAGAAAGAAATTTGGATTCCTCCAAAACATCTTTGA; TTGTAAAGAAAAAATTTGTTATCTTCAATAACCCCCAG;
• Restriction Sites: NotI-NotI
• ACCN: NM_152683
• Insert Size: 2250 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_152683.1, NP_689896.1
• RefSeq Size: 2078 bp
• RefSeq ORF: 1683 bp
• Locus ID: 201973
• UniProt ID: Q96LW4
• Cytogenetics: 4q35.1
• Summary: This gene encodes a DNA primase-polymerase that belongs to a superfamily of archaeao-eukaryotic primases. Members of this family have primase activity, catalyzing the synthesis of short RNA primers that serve as starting points for DNA synthesis, as well as DNA polymerase activity. The encoded protein facilitates DNA damage tolerance by mediating uninterrupted fork progression after UV irradiation and reinitiating DNA synthesis. An allelic variant in this gene is associated with myopia 22. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2016]