SIPA1 (NM_153253) Human Untagged Clone

SKU SC100541

SIPA1 (untagged)-Human signal-induced proliferation-associated 1 (SIPA1), transcript variant 1

Product Data

• Type: Human Untagged Clone
• Target Symbol: SIPA1
• Synonyms: SPA1
• Vector: pCMV6-XL6
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC100541 sequence for NM_153253 edited (data generated by NextGen Sequencing); ATGCCCATGTGGGCCGGCGGTGTGGGGAGCCCTCGGCGGGGCATGGCCCCTGCGTCCACA; GATGACCTCTTTGCCCGCAAGCTGCGCCAGCCAGCAAGGCCCCCGCTGACACCGCACACC; TTCGAGCCGAGGCCAGTCCGGGGCCCACTCCTGCGCAGCGGCAGCGATGCAGGCGAGGCC; AGGCCCCCCACGCCAGCCAGCCCCCGTGCCCGTGCCCACAGCCACGAAGAGGCCAGCCGA; CCTGCAGCCACTTCCACCCGGCTCTTCACTGACCCGCTGGCACTGCTGGGGCTGCCAGCA; GAGGAACCAGAGCCTGCCTTCCCACCAGTGCTTGAGCCTCGATGGTTTGCCCACTATGAC; GTGCAAAGCCTGCTCTTTGATTGGGCTCCGAGGTCTCAGGGGATGGGGAGCCACTCAGAG; GCCAGCTCTGGGACCCTGGCTTCAGCCGAGGACCAGGCTGCCAGCTCGGACCTGCTGCAT; GGGGCACCTGGCTTTGTGTGTGAGCTCGGGGGTGAGGGTGAGCTAGGCCTGGGTGGACCA; GCATCCCCACCTGTGCCCCCTGCACTGCCCAACGCGGCCGTGTCCATCCTGGAGGAGCCA; CAGAACCGAACCTCGGCCTACAGCCTGGAGCACGCAGACCTGGGTGCTGGCTACTACCGC; AAATACTTCTATGGCAAAGAACATCAGAACTTCTTCGGGATGGACGAGTCGCTGGGCCCG; GTGGCAGTGAGCCTGCGGCGGGAGGAGAAGGAGGGCAGCGGAGGGGGCACCCTGCACAGC; TACCGCGTCATCGTGCGGACCACGCAGCTCCGGACACTCCGTGGCACCATCTCGGAGGAC; GCGCTGCCGCCGGGGCCCCCACGGGGTCTGTCCCCAAGGAAACTTCTGGAGCACGTGGCG; CCGCAGCTGAGCCCCAGCTGCCTGCGCCTGGGCTCAGCTTCACCCAAGGTACCACGGACG; CTGCTCACACTGGATGAGCAAGTGCTGAGCTTCCAACGCAAGGTGGGCATCCTGTACTGC; CGGGCGGGCCAGGGCTCGGAGGAGGAGATGTACAACAACCAGGAGGCGGGACCGGCCTTC; ATGCAGTTTCTCACCTTGCTGGGCGATGTGGTGCGGCTCAAAGGCTTTGAGAGTTACCGG; GCCCAGCTAGACACCAAAACGGATTCCACAGGCACGCACTCCCTCTACACCACATACCAG; GACCACGAGATCATGTTCCACGTGTCCACGATGCTGCCTTACACCCCTAATAACCAGCAG; CAGCTCCTCCGGAAGCGCCACATTGGCAACGACATTGTGACCATCGTGTTCCAGGAGCCT; GGCAGCAAGCCCTTCTGCCCCACCACCATCCGCTCGCACTTCCAGCACGTGTTCCTAGTG; GTGCGGGCACACACACCCTGCACGCCACACACCACCTACAGGGTGGCCGTGAGCCGCACC; CAGGACACCCCTGCCTTCGGGCCAGCTCTGCCTGCTGGCGGAGGCCCCTTCGCAGCCAAC; GCCGACTTCCGGGCCTTCCTGCTGGCCAAAGCGCTGAATGGTGAGCAGGCGGCCGGCCAC; GCGCGCCAGTTCCACGCCATGGCCACGCGCACCCGCCAGCAGTACCTGCAAGACCTGGCC; ACCAACGAGGTGACCACTACGTCGCTGGACTCGGCTTCACGCTTCGGCCTGCCCTCCCTG; GGTGGGAGGCGCCGGGCGGCCCCTCGGGGCCCAGGCGCCGAGCTGCAGGCAGCGGGCTCA; CTGGTGTGGGGAGTGCGCGCGGCGCCCGGGGCGCGGGTCGCCGCCGGGGCTCAGGCGAGC; GGCCCCGAAGGCATCGAGGTGCCCTGCCTGCTGGGCATCTCGGCCGAGGCTCTGGTGCTG; GTGGCGCCGCGCGACGGCCGCGTAGTGTTCAATTGCGCCTGTCGCGACGTGCTGGCCTGG; ACCTTCTCCGAGCAGCAGCTGGACCTGTACCACGGCCGCGGGGAGGCGATCACGCTGCGC; TTCGACGGGTCCCCCGGCCAAGCCGTGGGCGAGGTGGTGGCGCGCCTGCAGCTGGTGAGC; CGTGGCTGCGAGACCCGCGAGCTGGCGCTGCCCCGCGACGGTCAAGGCCGCCTGGGCTTC; GAGGTGGACGCCGAGGGATTCGTCACGCACGTGGAGCGCTTCACATTCGCCGAGACGGCG; GGGCTGCGGCCCGGGGCGCGCCTCCTGCGCGTGTGCGGCCAGACGCTGCCCAGCCTCCGG; CCCGAGGCCGCTGCCCAGCTCCTGCGCTCGGCGCCCAAGGTCTGCGTCACCGTCCTGCCC; CCCGACGAGAGCGGCCGGCCCCGCAGGAGTTTTTCGGAGCTGTACACGCTGTCGCTGCAG; GAGCCTAGCCGGCGGGGGGCCCCAGATCCTGTGCAGGATGAGGTCCAGGGGGTGACCCTG; CTGCCCACCACAAAGCAGCTGCTGCACCTGTGCCTGCAAGATGGTGGCAGTCCTCCAGGG; CCTGGGGATCTGGCCGAGGAGAGGACTGAGTTCCTGCACAGCCAGAACTCGCTGTCACCA; CGCAGCTCTCTGTCGGATGAGGCCCCAGTCCTGCCCAACACCACCCCGGACCTCCTCCTG; GCCACCACAGCCAAGCCATCAGTACCCAGTGCTGACAGTGAGACACCCCTGACCCAGGAC; AGGCCAGGCAGTCCCAGTGGCTCTGAGGACAAGGGCAACCCGGCGCCGGAGCTGAGGGCC; TCCTTTCTGCCACGTACCTTGTCTCTGCGGAACTCCATCAGCAGGATCATGTCGGAGGCG; GGCAGTGGGACCCTGGAGGACGAGTGGCAGGCCATCTCGGAGATTGCCTCTACTTGCAAC; ACCATTCTGGAGTCGCTGTCCCGAGAGGGACAGCCCATCCCAGAGAGTGGAGACCCTAAG; GGAACTCCAAAATCTGATGCTGAGCCAGAGCCTGGGAACCTCTCAGAGAAGGTCTCTCAC; TTGGAGTCCATGCTCAGGAAGCTGCAGGAGGACCTGCAGAAGGAGAAGGCGGACAGGGCG; GCCCTGGAGGAGGAGGTGCGGAGCCTGAGACACAACAACCGGCGGCTGCAGGCGGAGTCT; GAGAGTGCAGCCACACGCCTCCTCCTGGCCTCCAAGCAGCTGGGCTCACCCACCGCCGAC; CTGGCCTGA; ; Clone variation with respect to ;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_153253 unedited; TAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCATTTAGGTGACACTATAGAATA; CAAGCTACTTGTTCTTTTTGCAGCGGCCGCGAATTCGGCACGAGGGCGGGACCCCAGCGC; GGGCGGCGGCGGCTGGGCGGGAGCCCCAGAGGGCAGGAACTGCTGCCACAACCTCAGGCT; GGGCACCAAACACCCGTGCCCGCCAATGCGGCCCAGCCCCCGGAGAGTCAGGCCCACAGA; GCATGCCCATGTGGGCCGGCGGTGTGGGGAGCCCTCGGCGGGGCATGGCCCCTGCGTCCA; CAGATGACCTCTTTGCCCGCAAGCTGCGCCAGCCAGCAAGGCCCCCGCTGACACCGCACA; CCTTCGAGCCGAGGCCAGTCCGGGGCCCACTCCTGCGCAGCGGCAGCGATGCAGGCGAGG; CCAGGCCCCCCACGCCAGCCAGCCCCCGTGCCCGTGCCCACAGCCACGAAGAGGCCAGCC; GACCTGCAGCCACTTCCACCCGGCTCTTCACTGACCCGCTGGCACTGCTGGGGCTGCCAG; CAGAGGAACCAGAGCCTGCCTTCCCACCAGTGCTTGAGCCTCGATGGTTTGCCCACTATG; ACGTGCAAAGCCTGCTCTTTGATTGGGCTCCGAGGTCTCAGGGGATGGGGAGCCACTCAG; AGGCCAGCTCTGGGACCCTGGCTTCAGCCGAGGACCAGGCTGCCAGCTCGGACCTGCTGC; A;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_153253 unedited; GAAAATCTATGNNACCGCGGCCGCAATTTANGATCGAGTTTTTTTTTTTTTTTTTTCAGT; GGCTTTTTTACTCCCTGAAGTCCTCTTTTCTCCACAGAATATTTACAAACAGGGAAGGGG; TGGAGGCCACTTCCGCCACCAAATAAGGGGCTAGGGAGGGGGGCAGTGCTAAGACACGCC; TCTGCGCAGGGAGAGTTCCTGAGGAGGGCCCAGTGTGACCACAGTGCCCTCAGGGGCCCA; GGTGGTTCCAGACGGCTCAGGCCAGGTCGGCGGTGGGTGAGCCCAGCTGCTTGGAGGCCA; GGAGGAGGCGTGTGGCTGCACTCTCAGACTCCGCCTGCAGCCGCCGGTTGTTGTGTCTCA; GGCTCCGCACCTCCTCCTCCAGGGCCGCCCTGTCCGCCTTCTCCTTCTGCAGGTCCTCCT; GCAGCTTCCTGAGCATGGACTCCAAGTGAGAGACCTTCTCTGAGAGGTTCCCAGGCTCTG; GCTCAGCATCAGATTTTGGAGTTCCCTTAAGGTCTCCACTCTCTGGGATGGGCTGTCCTG; TGGGGACAAAAGGATCAGGTGGGCAGGTCAAAAAGGGGCAAGAACACTGCCCAGGCTTAC; CGGAACCCCCTGTTGGGCTCCCCCTCTCGGACAACGACTCCCAAAAGGTGGTTCCAAGTA; AAGGCAATCTTCCGAGATGGCCTGCCCTTCGTCCTCCAAGGTCCCCCTGGCGCCGCCCTC; GACTAGAACCCCGCGGAAGGAGTTTCCCCAAAAACAAGGGACGTTGGCCAAAAAGAGAGC; CCTAAATTTCGGGCCGGGGTTGGCTTTGTCTTAAAAACCCACGAAAAAGCTGGGCCGGTC; TCGGGTAAGGGGGGGGTCCACTTCTAACCCGGGGACTAAAGGCCGGCGTCTATGGACCTC; AAAAAAGTCCGGGGGT;
• Restriction Sites: NotI-NotI
• ACCN: NM_153253
• Insert Size: 3900 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_153253.28, NP_694985.28
• RefSeq Size: 3657 bp
• RefSeq ORF: 3129 bp
• Locus ID: 6494
• UniProt ID: Q96FS4
• Cytogenetics: 11q13.1
• Domains: PDZ, Rap_GAP
• Protein Families: Druggable Genome, ES Cell Differentiation/IPS
• Protein Pathways: Leukocyte transendothelial migration
• Summary: The product of this gene is a mitogen induced GTPase activating protein (GAP). It exhibits a specific GAP activity for Ras-related regulatory proteins Rap1 and Rap2, but not for Ran or other small GTPases. This protein may also hamper mitogen-induced cell cycle progression when abnormally or prematurely expressed. It is localized to the perinuclear region. Two alternatively spliced variants encoding the same isoform have been characterized to date. [provided by RefSeq, Jul 2008]; Transcript Variant: This variant (1) represents the longer transcript. Variants 1 and 2 encode the same isoform.