KLHL9 (NM_018847) Human Untagged Clone

SKU SC100463

KLHL9 (untagged)-Human kelch-like 9 (Drosophila) (KLHL9)

Product Data

• Type: Human Untagged Clone
• Target Symbol: KLHL9
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC100463 sequence for NM_018847 edited (data generated by NextGen Sequencing); ATGAAAGTGTCCCTTGGTAACGGCGAAATGGGCGTCTCTGCCCATTTGCAGCCTTGTAAG; GCAGGAACCACACGCTTTTTTACCAGCAATACTCACAGTTCGGTGGTATTGCAAGGCTTT; GATCAGCTTAGAATAGAAGGATTGCTTTGTGATGTGACCCTGGTACCAGGTGATGGAGAT; GAAATCTTCCCTGTTCACAGAGCTATGATGGCGTCTGCTAGTGATTATTTCAAAGCCATG; TTCACAGGTGGAATGAAAGAACAAGATTTGATGTGCATTAAGCTTCATGGGGTGAACAAG; GTTGGTCTGAAGAAAATCATTGATTTTATTTATACTGCAAAACTTTCTCTTAATATGGAC; AATCTTCAGGACACACTTGAAGCTGCTAGCTTTTTACAAATATTACCCGTTTTGGATTTC; TGTAAAGTATTTCTTATATCAGGAGTCTCTTTGGATAACTGTGTTGAGGTTGGACGAATT; GCTAACACCTACAATCTTATAGAAGTGGATAAATATGTTAATAATTTCATCCTGAAGAAC; TTTCCTGCTTTATTGAGTACTGGGGAGTTTCTAAAACTCCCTTTTGAACGACTTGCATTT; GTGCTTTCCAGTAATAGTCTTAAGCACTGTACCGAACTTGAACTCTTTAAGGCAGCCTGT; CGCTGGCTAAGGTTGGAAGACCCTCGGATGGATTATGCTGCAAAGTTAATGAAGAATATT; CGATTTCCACTGATGACACCACAGGATCTCATCAATTACGTGCAGACAGTAGATTTCATG; AGAACAGACAATACCTGCGTGAATTTGCTTTTGGAAGCTAGCAATTACCAAATGATGCCA; TATATGCAGCCAGTGATGCAGTCAGATAGAACTGCCATTCGATCTGACTCCACTCACTTG; GTTACATTAGGAGGAGTTTTGAGGCAGCAGCTGGTTGTCAGTAAAGAATTACGGATGTAT; GATGAAAGGGCACAAGAATGGAGATCTTTAGCCCCAATGGATGCTCCCCGTTACCAGCAT; GGTATTGCTGTCATTGGAAACTTTCTTTATGTAGTTGGTGGTCAGAGTAATTATGATACA; AAAGGAAAAACTGCTGTTGATACAGTTTTCAGATTTGATCCTCGGTATAATAAATGGATG; CAGGTTGCATCATTAAATGAAAAGCGCACATTCTTTCACTTGAGTGCCCTCAAAGGACAT; TTGTATGCAGTTGGTGGGCGCAGTGCAGCTGGTGAACTGGCCACAGTAGAATGTTACAAC; CCAAGAATGAATGAGTGGAGCTATGTTGCAAAAATGAGTGAACCCCACTATGGTCATGCT; GGAACAGTATATGGAGGCTTAATGTATATTTCAGGAGGAATTACCCATGACACTTTCCAA; AATGAGCTCATGTGTTTTGACCCAGATACAGATAAATGGATGCAAAAGGCTCCAATGACT; ACAGTCAGAGGTCTGCATTGCATGTGTACAGTTGGAGATAAGCTCTATGTCATTGGTGGC; AATCACTTCAGAGGAACAAGTGATTATGATGATGTTCTAAGCTGTGAATACTATTCACCA; ACCCTTGACCAGTGGACCCCAATTGCCGCCATGTTAAGAGGCCAAAGTGATGTTGGAGTT; GCCGTCTTTGAAAATAAAATCTATGTTGTTGGTGGATATTCTTGGAATAATCGTTGTATG; GTAGAAATTGTCCAGAAATATGACCCAGAAAAAGATGAGTGGCATAAAGTTTTTGATCTT; CCAGAGTCACTTGGTGGCATTCGAGCCTGTACACTCACAGTTTTTCCACCTGAAGAAAAC; CCTGGGTCACCTTCTAGAGAATCACCTCTTTCAGCACCTTCAGATCATTCTTAG; ; Clone variation with respect to NM_018847.2;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_018847 unedited; GGGGTGTTCACCATTTTGTAATACGACTCATATAGGCGGCCGCGNATTCGGCACGAGGGT; TGGGGCTAGGCCGGTATTCTAGGCCCAAGCCCAGGCCCAGGCCTGTGTTCCCGGCCCTTG; CTCAAAGACAGGCCGGTGGCCGCTGCGACACTCTAGGTACAGCGGAGGGCGCAGCGGGCC; GTTCGCTGAGGTGTCCGGACCCGCCGCCGAGCGGGCCGTGGAGCCACAGTACGTGGCGGC; GCCTCTTCCTTGCCCGAAGCGGAACTGCTGAAGGAGCGGCAGCGCAGGCCGGACGAGGTG; AACCAGGTCATTTAACCGCCTTCAGGTGATGAATCATCTGTCTAATAATCAGATTTGAGG; TCCACAGCCCTTTGTGGTCCTGCTACAGGGTGATTTTCAGTGGATGACTGACTGGCGTGG; CCCGAGGGGTGCCGCAGTGACTGCCTTCCCTTGATAAGGAAACGAGCTTTCTGTTCTTTC; TGGACACCTCGTTACCTGTAAAACATTCCGGTTATAACTTCTTTATCCGGCTTGCATCTC; TGTGCAAGAGGAAAGCTTTCACATGAAAGTGTCCCTTGGTAACGGCGAAATGGGCGTCTC; TGCCCATTTGCAGCCTTGTAAGGCAGGAACCACACGCTTTTTTACCAGCAATACTCACAG; TTCGGTGGTATTGCAAGGCTTTGATCAGCTTAGAATAGAAGGATTGCTTTGTGATGTGAC; CCTGGTACCAGGTGATGGAGATGAAAATCTTCCTGTTCACAGAGCTATGATGGCGTCTGC; TAGTGATTATTTCAAAGCCATGTTCACCAGTGGGAATGAAGAACCAGATTTGATGTGCAT; TAAGCTTCATGGGGTGAACAAGGGTTGTCTGAAGAAATTATTGGTTTTATTTATACCGCA; AAACTTCTTTTTATATGAN;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_018847 unedited; TTTTTTTTTTTTTTTTTTTATGCAAGTTAAATTACATTTATTATATAAAGGGATCCTATA; ACTTGATACGAAAAACAAAGCAACTCCAACAGATAACAGAAGGGCAAAAGGACAGGAACA; TCTGATCAAAGAAACACAGCTACCGATAGCACACAAATATTCAACCTCATTAATAATCAA; AGGATTAGGATGCACTTCTTGCTTATTCAATAAAGTTAATAATTTCTAATTTTTCTACTT; TTCAAATGTACTCAAATGTGCTATTTTTAGTAATAAAAAACTGAGTAATTAAAAAAACAT; AGAAAGTATGAAAATTTCTGCCAATGCAGAAATCATAAACAGCATTAAAATGAATCAACA; CTTGTATGGGCAGTAAGGTTCAGACCCCTAGAAGCCAATTCATTTTGCCTTGGTTCCTGA; GTTTTATTATGGGATTGTCAATAAGGAGAAAGTTGTTCCTGATTTACATGCTGACAATCT; TCCAGGT;
• Restriction Sites: NotI-NotI
• ACCN: NM_018847
• Insert Size: 4700 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_018847.1, NP_061335.1
• RefSeq Size: 4373 bp
• RefSeq ORF: 1854 bp
• Locus ID: 55958
• UniProt ID: Q9P2J3
• Cytogenetics: 9p21.3
• Domains: BTB, Kelch
• Protein Pathways: Ubiquitin mediated proteolysis
• Summary: This gene encodes a protein that belongs to the kelch repeat-containing family, and contains an N-terminal BTB/POZ domain, a BACK domain and six C-terminal kelch repeats. The encoded protein is a component of a complex with cullin 3-based E3 ligase, which plays a role in mitosis. This protein complex is a cell cycle regulator, and functions in the organization and integrity of the spindle midzone in anaphase and the completion of cytokinesis. The complex is required for the removal of the chromosomal passenger protein aurora B from mitotic chromosomes. [provided by RefSeq, Jul 2016]