PI 3 Kinase regulatory subunit 4 (PIK3R4) (NM_014602) Human Untagged Clone

SKU SC100382

PIK3R4 (untagged)-Human phosphoinositide-3-kinase, regulatory subunit 4 (PIK3R4)

Product Data

• Type: Human Untagged Clone
• Target Symbol: PI 3 Kinase regulatory subunit 4
• Synonyms: p150; VPS15
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_014602 edited; CGCGAATTCGGCACGAGGGTTGCTTTCCCGGGAGTTCGGCGTTTGCTGGGGCTGCAGCAG; CTGAAGTGTAGTGTTTTCTTGGGACTGGCGGTCTGCACTTCTCTCCCGGGTTCCATCTCC; CCCCGCCCGGTGGTGAGGCCCTCGAGGAGGGCTCGGACGGGTGTAGCGATCCGCGCTAGA; GGAAGACGAGGCCCGGGAACGCATGTCCCCCAGGGCAGGTTAGGGGGCTGGAGGGGTCAA; ATCCCGGGGTACTTGTGGAGACTCTTTAGCGTGGCTTCTTCTCTCTGCTGAGACCCCGAG; AGCTTTCCCAGTTCTCCTCCCAGGACCACCGGGGTTCCTGAAGATCGGGACTTTTCTGCG; CCCCTCCACCAACAGCCCATCTCCTGTCTATGAAGAAAGACCCTTCGTAGAAACAACTTC; CCCGCTGCTGACGCGTTTTCCCGTCCCGTCCCCGAAGTAGTCTACTATGACCTCGTTGTG; AGCCTCTGAACGATTTTGACACTTTCCCGAGGCCTAGGGTATTATATCCTAACCTTACTA; AAGACCACAGAGGTGCTTGCCATTATGGGAAATCAGCTTGCTGGCATTGCTCCCTCCCAG; ATCCTTTCTGTAGAGAGTTATTTTTCAGATATTCATGACTTTGAATATGATAAAAGCCTG; GGGAGTACTCGGTTTTTTAAAGTTGCTCGAGCCAAGCACCGAGAAGGCCTGGTCGTTGTG; AAGGTTTTTGCAATTCAGGATCCCACATTGCCTTTAACCAGCTATAAACAAGAGCTGGAG; GAACTGAAAATCAGGCTTAATTCTGCACAGAATTGTCTACCTTTCCAGAAAGCATCAGAA; AAAGCATCTGAGAAAGCAGCTATGCTCTTTAGGCAGTATGTGCGAGACAATCTCTATGAT; CGCATCAGTACCCGTCCATTCTTGAATAACATTGAGAAGCGCTGGATTGCTTTCCAGATC; CTGACAGCTGTGGACCAAGCACACAAATCTGGAGTTCGTCATGGGGACATCAAGACTGAG; AATGTGATGGTCACCAGTTGGAATTGGGTTCTTCTAACTGATTTTGCCAGTTTTAAGCCC; ACTTATCTTCCAGAAGACAACCCGGCAGATTTCAATTATTTCTTTGACACATCACGGAGG; AGAACTTGCTATATTGCTCCTGAACGTTTTGTTGATGGTGGGATGTTTGCCACTGAGTTA; GAATATATGAGAGATCCTTCAACTCCGCTTGTAGACTTAAATAGCAATCAGAGAACAAGA; GGAGAGTTGAAGAGAGCAATGGACATCTTTTCAGCAGGTTGTGTGATAGCTGAGCTTTTT; ACAGAAGGTGTACCATTATTTGATCTCTCTCAACTTTTGGCTTATAGAAATGGACATTTT; TTCCCTGAACAAGTGCTAAATAAAATTGAAGATCACAGTATCAGAGAATTGGTAACTCAG; ATGATTCACCGTGAGCCAGATAAACGTTTAGAGGCAGAAGATTACTTAAAACAGCAGCGT; GGCAATGCCTTTCCTGAAATATTTTACACTTTTCTTCAGCCCTACATGGCCCAGTTTGCC; AAGGAAACGTTTCTTTCTGCAGATGAGCGTATTCTGGTTATACGGAAGGATTTGGGCAAC; ATTATTCACAATCTCTGTGGACATGATCTGCCAGAAAAAGCCGAAGGAGAGCCTAAGGAA; AATGGGCTGGTTATCTTGGTATCTGTTATAACATCCTGCCTACAGACCCTTAAATACTGT; GATTCCAAACTAGCTGCTTTGGAACTGATTCTTCATTTGGCTCCAAGATTAAGTGTTGAA; ATCCTTTTGGATCGTATTACTCCATATCTTTTGCATTTCAGCAATGACTCTGTTCCTAGG; GTGAGGGCTGAAGCCTTGAGGACGTTGACCAAAGTTCTTGCTCTCGTCAAAGAGGTTCCT; CGTAATGATATCAATATTTATCCGGAATACATTCTGCCAGGCATAGCCCACTTAGCCCAA; GATGATGCTACTATCGTTAGACTAGCCTATGCTGAAAACATAGCTCTGCTGGCAGAAACA; GCTCTGAGATTCCTGGAATTAGTACAGTTAAAAAATCTTAATATGGAAAATGACCCCAAT; AATGAAGAAATAGATGAGGTTACACATCCAAATGGAAATTATGACACAGAGCTCCAAGCC; TTACATGAAATGGTCCAGCAGAAAGTTGTTACTTTGCTAAGTGACCCTGAAAATATTGTA; AAACAAACCTTGATGGAAAATGGAATAACACGGCTGTGTGTATTCTTTGGACGTCAGAAA; GCCAACGATGTTTTGTTGTCCCACATGATTACTTTCCTAAATGATAAGAATGATTGGCAT; CTACGTGGAGCATTTTTTGATAGTATAGTTGGTGTTGCTGCCTATGTTGGCTGGCAAAGC; TCCTCAATTCTCAAGCCTCTGCTGCAACAAGGTCTTAGTGATGCTGAGGAATTTGTCATT; GTGAAAGCTCTTTATGCCCTTACTTGTATGTGCCAGTTAGGACTGCTACAAAAACCCCAT; GTTTACGAATTTGCCAGTGATATTGCCCCCTTCCTGTGTCATCCCAATTTATGGATACGT; TATGGTGCCGTGGGATTTATCACAGTGGTAGCTCGTCAAATAAGTACAGCTGATGTCTAC; TGTAAACTGATGCCTTATCTTGACCCATATATTACCCAACCAATAATACAGATTGAAAGA; AAACTTGTTCTGCTCAGTGTTTTAAAGGAACCAGTAAGTCGTTCTATATTTGATTATGCT; TTGAGGTCTAAAGATATTACTAGCTTGTTCAGACATCTTCACATGCGTCAGAAGAAACGA; AATGGTTCTCTTCCCGACTGCCCTCCGCCAGAGGATCCTGCCATAGCACAGCTTCTGAAG; AAGTTGCTCTCACAGGGAATGACAGAGGAAGAGGAAGACAAACTTCTGGCACTGAAAGAC; TTCATGATGAAATCTAATAAAGCAAAGGCCAATATAGTGGACCAGAGCCATCTTCATGAT; AGTAGTCAGAAAGGTGTAATTGACTTGGCAGCTTTAGGCATAACTGGGAGACAAGTTGAT; CTTGTTAAAACCAAACAAGAACCAGATGACAAACGGGCCAGAAAACATGTAAAACAAGAC; TCAAATGTAAATGAAGAATGGAAAAGCATGTTTGGGTCACTGGACCCACCAAACATGCCA; CAGGCCCTACCTAAAGGGAGTGATCAGGAGGTGATTCAGACTGGGAAACCTCCTCGTTCC; GAGTCCTCTGCTGGCATTTGTGTCCCTTTGTCAACTTCTTCACAGGTTCCAGAAGTGACA; ACTGTCCAAAATAAAAAACCAGTAATACCGGTTTTAAGTAGTACAATCTTACCATCCACC; TATCAGATTCGAATTACAACTTGTAAAACTGAACTTCAGCAACTCATCCAGCAAAAGCGG; GAGCAGTGCAATGCTGAGAGAATAGCTAAGCAGATGATGGAAAATGCTGAATGGGAGAGT; AAACCACCACCACCTGGATGGCGTCCTAAAGGGCTGTTAGTTGCCCATCTTCATGAGCAT; AAATCTGCTGTGAATCGAATTAGAGTCTCTGATGAACACTCACTTTTTGCAACATGTTCA; AATGATGGCACAGTGAAAATCTGGAACAGTCAAAAGATGGAGGGGAAGACCACCACTACC; AGATCTATTCTTACATACAGCCGAATTGGAGGACGAGTCAAGACGCTCACATTCTGCCAA; GGCTCCCACTATTTAGCCATAGCATCTGATAATGGTGCTGTCCAGCTTCTTGGAATTGAG; GCTTCTAAGCTGCCCAAGTCTCCTAAAATCCATCCTCTACAAAGCAGAATTCTAGATCAG; AAGGAGGACGGTTGTGTTGTGGATATGCATCACTTCAACTCTGGAGCACAGTCTGTTCTT; GCCTATGCCACTGTGAATGGCTCTCTGGTTGGCTGGGACCTTAGGTCTTCAAGCAATGCG; TGGACTTTAAAGCATGATTTAAAGTCGGGCCTCATCACTTCCTTTGCTGTGGACATCCAC; CAATGCTGGCTCTGCATTGGTACAAGCAGTGGTACCATGGCTTGTTGGGACATGAGGTTC; CAGTTGCCAATTTCAAGTCACTGTCATCCTTCCAGGGCTCGAATCAGACGCCTCTCAATG; CACCCTCTGTATCAGTCCTGGGTGATTGCAGCTGTTCAGGGCAACAACGAAGTGTCCATG; TGGGACATGGAGACTGGTGACAGAAGATTTACTCTCTGGGCCAGCAGTGCACCACCACTT; TCTGAATTACAGCCTTCTCCTCATAGCGTCCATGGTATCTACTGTAGTCCTGCAGATGGA; AATCCTATCCTACTAACAGCTGGCTCAGATATGAAAATAAGGTTTTGGGACTTGGCTTAC; CCAGAAAGGTCCTATGTTGTTGCAGGAAGTACTAGTTCCCCATCTGTGTCCTACTACAGG; AAAATAATTGAAGGCACTGAAGTTGTCCAGGAAATTCAGAATAAGCAGAAAGTAGGACCA; AGTGATGACACCCCTCGAAGGGGCCCAGAGTCCCTGCCCGTGGGACATCATGACATCATC; ACTGATGTCGCCACATTCCAGACCACACAGGGCTTCATCGTAACTGCTTCTAGAGATGGG; ATTGTGAAGGTGTGGAAATAAAACCTACTGATTTGTATAAATTTTAATAGTTATAAATAT; AATACTATAACTCGAGAAAAGGCATTTCTAGAGAACAGATTCATTTGCTTAATTTTCAAA; ATTATGTCTCCATATTACTGTTTCATGACTGACTGACTAAATGACACCCAAAATGGTTAA; GATGTACTTGACTAGTTTACTTATGCATCTCTTTGCAAGAATCAGCCAGCCAACAATGTC; TGGGATTTTTATTGTATATGTTATAGAGGTGAGAAATGTAAAATATGAAAATGAATATGT; TTATTTTGTATTGAAAA;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_014602 unedited; TGTAATACGACTTACTATAGGGCGGCCGCGAATTCGGCACGAGGGTTGCTTTCCCGGGAG; TTCGGCGTTTGCTGGGGCTGCAGCAGCTGAAGTGTAGTGTTTTCTTGGGACTGGCGGTCT; GCACTTCTCTCCCGGGTTCCATCTCCCCCCGCCCGGTGGTGAGGCCCTCGAGGAGGGCTC; GGACGGGTGTAGCGATCCGCGCTAGAGGAAGACGAGGCCCGGGAACGCATGTCCCCCAGG; GCAGGTTAGGGGGCTGGAGGGGTCAAATCCCGGGGTACTTGTGGAGACTCTTTAGCGTGG; CTTCTTCTCTCTGCTGAGACCCCGAGAGCTTTCCCAGTTCTCCTCCCAGGACCACCGGGG; TTCCTGAAGATCGGGACTTTTCTGCGCCCCTCCACCAACAGCCCATCTCCTGTCTATGAA; GAAAGACCCTTCGTAGAAACAACTTCCCCGCTGCTGACGCGTTTTCCCGTCCCGTCCCCG; AAGTAGTCTACTATGACCTCGTTGTGAGCCTCTGAACGATTTTGACACTTTCCCGAGGCC; TANGGTATTATATCCTAACCTTACTAAAGACCACAGAGGTGCTTGCCATTATGGGAAATC; AGCTTGCTGGCATTGCTCCCTCCCAAATCCTTTCTGTAGAGAGTTATTCCTCCGATATTC; ATGACTTTGAAAATGATAAAAACCCGCGGAGTACCTCCGGTTTTTAAAGCTGCTCGACCC; AATCACCGAAAGGCCCTGGCCGTTGTGAAAGTTTTTGAATTCAAGAACCCCAATTTGCCT; TCACCCCCTTTCAACAAAAAGCTGGAGGGAACCGCAAAATAAGGCCTCAATTCTGCCCCC; ACTCGCTCACTCTTTCCAAAATACTCCCAAAAACGCCCCCCCGAAATCCTCTCCCTCCTT; TTAGCCCTTTGTGCCATC;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_014602 unedited; TGGGTTTTCTATGNACGCGGCCGCATTCTANGATCGAGTTTTTTTTTTTTTTTTTTACAG; TTTAACTGCTTTGAATAGCATTTTTAATAGCAAAAATGTGTTTATACTATAATAGCTTAC; AACCATCTTTTCAACCATCTTTTTCAATACAAAATAAACATATTCATTTTCATATTTTAC; ATTTCTCACCTCTATAACATATACAATAAAAATCCCAGACATTGTTGGCTGGCTGATTCT; TGCAAAGAGATGCATAAGTAAACTAGTCAAGTACATCTTAACCATTTTGGGTGTCATTTA; GTCAGTCAGTCATGAAACAGTAATATGGAGACATAATTTTGAAAATTAAGCAAATGAATC; TGTTCTCTAGAAATGCCTTTTCTCGAGTTATAGTATTATATTTATAACTATTAAAATTTA; TACAAATCAGTAGGTTTTATTTCCACACCTTCACAATCCCATCTCTAGAAGCAGTTACGA; TGAAGCCCTGTGTGGTCTGGAATGTGGCGACATCAGTGATGATGTCATGATGTCCCACGG; GCAGGGACTCTGGGCCCCTTCGAGGGGTGTCATCACTTGGTCCTACTTTCTGCTTATTCT; GAATTTCCTGGACAACTTCAGTGCCTTCAATTATTTTCCTGTAGTAGGACACAGATGGGG; AACTAGTACTTCCTGCAACAACATAGGACCTTTCTGGGTAAGCCAAGTCCCAAAACCTTA; TTTTCATATCTGAGCCAGCTGTTAGTAGGATAGGATTTCCATCTGCAGGACTACAGTAGA; TACCATGGACGCTATGAGGAGAAGGCTGTAATTCAGAAAATGGTGGTGCACTGCTGGCCC; AGAGAGTAAATCTTCTGTCACCAGTCTCCATGTCCCACATGGACACTTCGTT;
• Restriction Sites: NotI-NotI
• ACCN: NM_014602
• Insert Size: 4700 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_014602.1, NP_055417.1
• RefSeq Size: 5060 bp
• RefSeq ORF: 4077 bp
• Locus ID: 30849
• UniProt ID: Q99570
• Cytogenetics: 3q22.1
• Domains: pkinase, S_TKc, WD40
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Regulation of autophagy
• Summary: Regulatory subunit of the PI3K complex that mediates formation of phosphatidylinositol 3-phosphate; different complex forms are believed to play a role in multiple membrane trafficking pathways: PI3KC3-C1 is involved in initiation of autophagosomes and PI3KC3-C2 in maturation of autophagosomes and endocytosis. Involved in regulation of degradative endocytic trafficking and cytokinesis, probably in the context of PI3KC3-C2 (PubMed:20643123).[UniProtKB/Swiss-Prot Function]