GPR73B (PROKR2) (NM_144773) Human Untagged Clone

SKU SC100222

PROKR2 (untagged)-Human prokineticin receptor 2 (PROKR2)

Product Data

• Type: Human Untagged Clone
• Target Symbol: GPR73B
• Synonyms: dJ680N4.3; GPR73b; GPR73L1; GPRg2; HH3; KAL3; PKR2
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >SC100222 representing NM_144773.; Blue=Insert sequence Red=Cloning site Green=Tag(s); ; GAATTCGGCACGAGGCTGGCTCCAAGCAGACATCACCATGGCAGCCCAGAATGGAAACACCAGTTTCAC; ACCCAACTTTAATCCACCCCAAGACCATGCCTCCTCCCTCTCCTTTAACTTCAGTTATGGTGATTATGA; CCTCCCTATGGATGAGGATGAGGACATGACCAAGACCCGGACCTTCTTCGCAGCCAAGATCGTCATTGG; CATTGCACTGGCAGGCATCATGCTGGTCTGCGGCATCGGTAACTTTGTCTTTATCGCTGCCCTCACCCG; CTATAAGAAGTTGCGCAACCTCACCAATCTGCTCATTGCCAACCTGGCCATCTCCGACTTCCTGGTGGC; CATCATCTGCTGCCCCTTCGAGATGGACTACTACGTGGTACGGCAGCTCTCCTGGGAGCATGGCCACGT; GCTCTGTGCCTCCGTCAACTACCTGCGCACCGTCTCCCTCTACGTCTCCACCAATGCCTTGCTGGCCAT; TGCCATTGACAGATATCTCGCCATCGTTCACCCCTTGAAACCACGGATGAATTATCAAACGGCCTCCTT; CCTGATCGCCTTGGTCTGGATGGTGTCCATTCTCATTGCCATCCCATCGGCTTACTTTGCAACAGAAAC; GGTCCTCTTTATTGTCAAGAGCCAGGAGAAGATCTTCTGTGGCCAGATCTGGCCTGTGGATCAGCAGCT; CTACTACAAGTCCTACTTCCTCTTCATCTTTGGTGTCGAGTTCGTGGGCCCTGTGGTCACCATGACCCT; GTGCTATGCCAGGATCTCCCGGGAGCTCTGGTTCAAGGCAGTCCCTGGGTTCCAGACGGAGCAGATTCG; CAAGCGGCTGCGCTGCCGCAGGAAGACGGTCCTGGTGCTCATGTGCATTCTCACGGCCTATGTGCTGTG; CTGGGCACCCTTCTACGGTTTCACCATCGTTCGTGACTTCTTCCCCACTGTGTTCGTGAAGGAAAAGCA; CTACCTCACTGCCTTCTACGTGGTCGAGTGCATCGCCATGAGCAACAGCATGATCAACACCGTGTGCTT; CGTGACGGTCAAGAACAACACCATGAAGTACTTCAAGAAGATGATGCTGCTGCACTGGCGTCCCTCCCA; GCGGGGGAGCAAGTCCAGTGCTGACCTTGACCTCAGAACCAACGGGGTGCCCACCACAGAAGAGGTGGA; CTGTATCAGGCTGAAGTGACCCACTGGTGTCACACAATTGAAAACCCCAGTCCAGTACTCAGAGCATCA; CCCACCATCAACCAAGTTCATAGGCTGCATGGGAAATGACATCTGTGTTCATGCCTCCCCCGTGCCCTC; AAGAAGCTGAATGCTGCAAAGTCGTAACATACAATGAGACTAGACATGAACCAAATCAGCTGACATTTA; CTGATATCCGCTCGACACCTACTGTGTCCACAATCCCAACAAGGAGATTAGACACAAGGAGCAGCAACT; GACATGGACTGAACATGTACTGTGTGCAAACCACACCAATGAGATTAGACAGGGACAGCAGGAGCTGAC; ATTTACTCTTCACCTACTGTAATAAAAAACACTTGATTTGATTACAATCAAAAACATATAAAAAACATA; ACAAAGTAGCAGAAGCTATTGGAGTTTCCAAGCTATCTCCAGATATATAGATAGTTCACCCTCCATCTT; CCCTAATTCTGTATCTTACCAGTGCAGGAATATCAAAAGGCTATAGGCCAGGCATGATGGCTCATGCCT; GTAATCCCAGCACTTGGGGAGGCTGAGGCACGTGGAACACTTGAGGTCAGGAGTTCAACCCAGGCTGGC; CAACATGGTGAAACCCTGTCTCTACTAAAAATACAAAATTAGCTAGGCGTGGTGGCGGGCGCCTGTAAT; CCCAGTTACTCAGGAGGCTGAAGCAGGAGAATAGCTTGAACCTGGGAGTTGGAGTTTGCAGTGAGCTGA; GATTGCTCCACTGCACTCCAGCCTGAGTGACAGAGTGAGACTCTGTCTCAGGAAAAAAACAAACAAACA; AACAACAAAACAACAACAACAACAACCAACGGCTATAGAAGAAGACTCTTAGACACAATGGAAATGTAA; CGATAAGTTTGTCAGTGCGTGGTTTACAGCATCATGGGAGGTGTGTTACAGCCATCATACTGAACTTTC; CCACCCACCTCCTACTGCCTCCCAGGGCATTCTCTAGGATTTTGGCTTCAAGAAAAAAAAAATTCTTAT; AGTCAGCCCAGCCTTATGTGGTTATCCACAATGGTGTAATTTCAAAGGAAAGAACCTAAAAATCACTTT; CCCACTGATGCTTGAAAGCTTATCATTTTATTTGGGTGGAGATGGGTAATCCTGAGGTGTCAATTTTTG; CCTCCTCAGTGCAAAGGATTTCAGTGGCTCTGGGGTCAGGGGGAAAGAGGACAGAGAAAAAAGTGGAGG; TTGCCACTGGCAATGAACATAATCTCTGTGGGCATTTTGCTAAGGACTGGACCACTTTCTAGAACACTC; CCTCTTTTACAAAAGGAACTCTACCTAGAATCCAAAGACCTGGGTTCAGGTCCTAACTCTAAGACTCAA; GTCCTAAATTCATGATGTTTTCTCTCTGTGTCTCAGTTTTGCTTTAATGAAATGGCGATGATGAAAATA; TCCGCTCTTCATACCTTGCAAGACTGTTGGGAGAGCCCATTGAGGCCATGGTTTGTGAATGTGCTTTTC; AACTGTGCACACGATAAGAATGGAGAAGTGATATTGAACAGTTTATTTGGAGGGAGTTTATTTGGAAAC; CCCATCCACTGTGATTTATTAGAGAAATACCCACACTTTTTCATCCCTGTTCTTTGGATGAAAGACTCC; TGAAGACTTCACAGTGTACCTTGTCTACAGTGGGCCAAAAAGGGATCCCTGTTCTTGGTTATAATCTGG; GAAATTTAACCTCAGATTCTCAGTGACCCCAAGACTCTCAGCATCCCTGCGGTCTTAGAAGTGTTGACA; GTCCTCCCTGCATGTTGCAAAATAGCACCCTAGTGCTGCATAAATATCACTTCTGAATCTGTTTGTATT; ATACATTTGTGGTAACTGTAGGTACACGTCTTCATTTCTTCTTGATTCATTTTGATGTGGTAGCTATGC; AAATGGTACCTGGTTTGGGACTGACCCAACCATATTTGACCAATTCCTAATTTTTTATAGACAAGGAAT; TAATTGTTTGCTTGTTTGATTGTTTCTATTATTTGTTGATTTGTTTCTCTGACTGAAGTTTCAACCAAT; ATTTCTTTCTATCACCACCCAGCAGACTCACCTTCAGCCCAATCATTGTACTCTCAGAAAATGCAGGCC; GGCATGGTGGCTCACATCTGTAATCCCAGCACTTCGGGAGGCCAAGATGGGCAGATCACCTGGGGTCGG; GAGTTCGAGACCAGCCTGGCCAACATGGCCAAAAACCCCATCTCTAGAAAAATACAGAAATTAGCTGGC; GTGGTGGCACATGCCTGTGGTCCCAGCTCCTCAGGAGGCTGAGGCATGAGAATTGCTTGAACCCCGGAG; GCAGAGGTTGCAGTGAATTGAGATCACACCACTGCACTCCAGCCTGGGTGATAGAGCAAGATTCCATCT; CAAAAGGAAAATAAAAGAAAATGCAAACACACTATAATATTAGCCAAGCAAGTAACAATTTTAGGTTAT; GTACATTTCTATAAATATAATAATTGTATTTTTATTTATTATTCTATCCTGGCTCTTAGCCGAATCAGG; AGATTCTTTAGGAATGGACCATGTACCAGTCAAGTCTGTCAGCAGGATTCATCACCCTGTTCCTTTTTG; TCCTAGAATATACCAACTTCCTTTCATTGAAATTTAACTGAAAAAACTTTTGTAAATATCAGTGTGTAT; TTGTGATTTTCCAGTGATTAAAGTGTAATGTTGTTATCCAATTAAATAATTAACATGTGAAAAAAAAAA; AAAAAAAAAAAAAACTCGACTCTAGATTGCGGCCGC;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_144773 unedited; NATACGACTTCACTATAGGGCGGCCGCGATTCGGCACGAGGCTGGCTCCAAGCAGACATC; ACCATGGCAGCCCAGAATGGAAACACCAGTTTCACACCCAACTTTAATCCACCCCAAGAC; CATGCCTCCTCCCTCTCCTTTAACTTCAGTTATGGTGATTATGACCTCCCTATGGATGAG; GATGAGGACATGACCAAGACCCGGACCTTCTTCGCAGCCAAGATCGTCATTGGCATTGCA; CTGGCAGGCATCATGCTGGTCTGCGGCATCGGTAACTTTGTCTTTATCGCTGCCCTCACC; CGCTATAAGAAGTTGCGCAACCTCACCAATCTGCTCATTGCCAACCTGGCCATCTCCGAC; TTCCTGGTGGCCATCATCTGCTGCCCCTTCGAGATGGACTACTACGTGGTACGGCAGCTC; TCCTGGGAGCATGGCCACGTGCTCTGTGCCTNCGTCAACTACCTGCGCACCGTCTNCCTC; TACGTCTNCACAATGCCTTGCTGGCCATTGNCATTGACAGATATCTCGCCATCGTTCACC; CCTTGGAACCACGGATGAATTATCAAACGGCCTTCTTNCTGATCGCCTTGGTCTGGATGG; GGGTCCATTCTCATGGCCATCCATCGGCTTACTTTGCACAGAAAACGGTCTCTTTAATTG; TCAAGAGCCAGAGAGATCTTCTGTGGCCAGACTGGCCTGGGGATCAGCAGTTTACTACAA; GGCCTACTTCTTCTCATACTTGGGGCCAAGTCCGGGGCCCTGGGGGACCCAGGACCCTGG; GCTTTCCCAGGATTTCCCGGGGCCTTGGGTAAGGGCAGCCCCTGGGTTTCAAAA;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_144773 unedited; GGACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTCACATGT; TAATTATTTAATTGGATAACAACATTACACTTTAATCACTGGAAAATCACAAATACCCAC; TGATATTTACAAAAGTTTTTTCAGTTAAATTTCAATGAAAGGAAGTTGGTATATTCTAGG; ACAAAAAGGAACAGGGTGATGAATCCTGCTGACAGACTTGACTGGTACATGGTCCATTCC; TAAAGAATCTCCTGATTCGGCTAAGAGCCAGGATAGAATAATAAATAAAAATACAATTAT; TATATTTATAGAAATGTACATAACCTAAAATTGTTACTTGCTTGGCTAATATTATAGTGT; GTTTGCATTTTCTTTTATTTTCCTTTTGAGATGGAATCTTGCTCTATCACCCAGGCTGGA; GTGCAGTGGTGTGATCTCAATTCACTGCAACCTCTGCCTCCGGGGGTTCAAGCAATTCTC; ATGCCTCAGCCTCCTGAGGAGCTGGGACCACAGGCATGTGCCACCACGCCAGCTAATTTC; TGTATTTTTCTAGAGATGGGGTTTTTGGCCATGTTGGCCAGGCTGGTCTCGAACTCCCGA; CCCCAGGTGATCTGCCCATCTTGGCCTCCCGAATGCTGGGATTACAGATGTGAGCCACCA; TGCCGGCCTGCATTTTCTGAGAGTACAATGATTGGGCTGAAGGTGAATCTGCTGGGTGGT; GAAAAAAAAAATATTGGTTGAAACTTCGTTCCGAGAACCAATCCCCAATTATTAGAACAC; TCCAACCAGCAAACATTTATTTCTTGGTCATAAAAAAATAGAAATGGGTCAAATTGGTTG; GGGCAGTCCCCACACCAGTCCTTTTTGCTTGCTCCCCCTCCAATGGATCAGAAGAATGAA; GACTGGTCCTCCCGTCCCACAATGGTTTACCAACCGTTCCGATTGGATTTTTCCCGCCTG; GGTGCTTTTTGCACTGGCCGGGGGCTGTGAACCTTTTGACCCCGGGG;
• Restriction Sites: NotI-NotI
• ACCN: NM_144773
• Insert Size: 3969 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_144773.2
• RefSeq Size: 1155 bp
• RefSeq ORF: 3969 bp
• Locus ID: 128674
• UniProt ID: Q8NFJ6
• Cytogenetics: 20p12.3
• Domains: 7tm_1
• Protein Families: Druggable Genome, GPCR, Transmembrane
• MW: 148.7 kDa
• Summary: Prokineticins are secreted proteins that can promote angiogenesis and induce strong gastrointestinal smooth muscle contraction. The protein encoded by this gene is an integral membrane protein and G protein-coupled receptor for prokineticins. The encoded protein is similar in sequence to GPR73, another G protein-coupled receptor for prokineticins. [provided by RefSeq, Jul 2008]