DIRAS1 (NM_145173) Human Untagged Clone

CAT#: SC100097

DIRAS1 (untagged)-Human DIRAS family, GTP-binding RAS-like 1 (DIRAS1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: DIRAS1
• Synonyms: Di-Ras1; GBTS1; RIG
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC100097 sequence for NM_145173 edited (data generated by NextGen Sequencing)
  ATGCCGGAACAGAGTAACGATTACCGCGTGGTGGTGTTCGGGGCGGGCGGCGTGGGCAAG
  AGCTCGCTGGTGCTGCGCTTCGTGAAGGGCACGTTCCGCGACACCTACATCCCCACCATC
  GAGGACACCTACCGGCAGGTGATCAGCTGCGACAAGAGCGTGTGCACGCTGCAGATCACA
  GACACCACCGGCAGCCACCAGTTCCCGGCCATGCAGCGCCTGTCCATCTCCAAGGGCCAC
  GCCTTCATCCTGGTGTTCTCCGTCACCAGCAAGCAGTCGCTGGAGGAGCTGGGGCCCATC
  TACAAGCTCATCGTGCAGATCAAGGGCAGCGTGGAGGACATCCCCGTGATGCTCGTGGGC
  AACAAGTGCGATGAGACGCAGCGGGAGGTGGACACGCGCGAGGCGCAGGCGGTGGCCCAG
  GAGTGGAAGTGCGCTTTCATGGAGACCTCGGCCAAGATGAACTACAACGTCAAGGAGCTC
  TTCCAGGAGCTGCTGACGCTGGAGACGCGCCGGAACATGAGCCTCAACATCGACGGCAAG
  CGCTCCGGCAAGCAGAAGAGGACAGACCGCGTCAAGGGCAAATGCACCCTCATGTGA
  
  Clone variation with respect to NM_145173.3
  549 g=>c
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_145173 unedited
  CGACTCACTATAGGCGGCCGCGAATTCGCACGAGGGGGGACGGCGCGGGCGCGACTGCGG
  CGGCTCGGGCGGGCGGGGGGCGGCGGCGCCAGCGGACACCGGAGCGGGCAGGAGCAGCGG
  CCGCGGCGCCGCAGGGACCAGCGGGCCCAGGCTCGGTCTGGGGTTCTTGCAGCTGGCACC
  AGCCCTCCCGGCCCCGGCCGCCCACCCGCCGCGGGGAAACATGCCGGAACAGAGTAACGA
  TTACCGCGTGGTGGTGTTCGGGGCGGGCGGCGTGGGCAAGAGCTCGCTGGTGCTGCGCTT
  CGTGAAGGGCACGTTCCGCGACACCTACATCCCCACCATCGAGGACACCTACCGGCAGGT
  GATCAGCTGCGACAAGAGCGTGTGCACGCTGCAGATCACAGACACCACCGGCAGCCACCA
  GTTCCCGGCCATGCAGCGCCTGTCCATCTCCAAGGGCCACGCCTTCATCCTGGTGTTCTC
  CGTCACCAGCAAGCAGTCGCTGGAGGAGCTGGGGCCCATCTACAAGCTCATCGTGCAGAT
  CAAGGGCAGCGTGGAGGACATCCCCGTGATGCTCGTGGGCAACAAGTGCGATGAGACGCA
  GCGGGAGGTGGACACGCGCGAGGCGCACGCGGTGGCCCAGGAGTGGAAGTGCGCTTTCAT
  GGAGACCTCGGCCAAGATGAACTACAACGTCAAGGAGCTCTTCCAGNAGCTGCTGACGCT
  GGAGACGCGCCGGAACATGAGCCTCACATTGACGGCAAGCGCTNCGGCAAGCAGAAGAGA
  CAGACCGCGTCAGGGGCAAATGCACCCTCATGTGAGCCCGGACGCCCGGCTGGGCCGGTG
  GCACACCCAACTGACCCCGGTGGTTCCCCGAAACGACACACTTGCTGGACCCTCTGTTTT
  TTTATCCCCCTGGCCACCTTGGCGGTGAAACCGGGCCACA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_145173
• Insert Size: 3500 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_145173.1, NP_660156.1
• RefSeq Size: 3391 bp
• RefSeq ORF: 597 bp
• Locus ID: 148252
• UniProt ID: O95057
• Cytogenetics: 19p13.3
• Domains: ras, RAS, RHO, RAB
• Gene Summary: DIRAS1 belongs to a distinct branch of the functionally diverse Ras (see HRAS; MIM 190020) superfamily of monomeric GTPases.[supplied by OMIM, Apr 2004]