Stefin B (CSTB) (NM_000100) Human Untagged Clone

CAT#: SC100002

CSTB (untagged)-Human cystatin B (stefin B) (CSTB)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Stefin B
• Synonyms: CPI-B; CST6; EPM1; EPM1A; PME; STFB; ULD
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC100002 sequence for NM_000100 edited (data generated by NextGen Sequencing)
  ATGATGTGCGGGGCGCCCTCCGCCACGCAGCCGGCCACCGCCGAGACCCAGCACATCGCC
  GACCAGGTGAGGTCCCAGCTTGAAGAGAAAGAAAACAAGAAGTTCCCTGTGTTTAAGGCC
  GTGTCATTCAAGAGCCAGGTGGTCGCGGGGACAAACTACTTCATCAAGGTGCACGTCGGC
  GACGAGGACTTCGTACACCTGCGAGTGTTCCAATCTCTCCCTCATGAAAACAAGCCCTTG
  ACCTTATCTAACTACCAGACCAACAAAGCCAAGCATGATGAGCTGACCTATTTCTGA
  
  Clone variation with respect to NM_000100.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000100 unedited
  GCACATTTTGTAATACGACTCACTATAGGGGCGGCCGCGATTCGGCACGAGGCCTCGTGC
  CGAATTCGGCACGAGGGTCGCCGCCAAGATGATGTGCGGGGCGCCCTCCGCCACGCAGCC
  GGCCACCGCCGAGACCCAGCACATCGCCGACCAGGTGAGGTCCCAGCTTGAAGAGAAAGA
  AAACAAGAAGTTCCCTGTGTTTAAGGCCGTGTCATTCAAGAGCCAGGTGGTCGCGGGGAC
  AAACTACTTCATCAAGGTGCACGTCGGCGACGAGGACTTCGTACACCTGCGAGTGTTCCA
  ATCTCTCCCTCATGAAAACAAGCCCTTGACCTTATCTAACTACCAGACCAACAAAGCCAA
  GCATGATGAGCTGACCTATTTCTGATCCTGACTTTGGACAAGGCCCTTCAGCCAGAAGAC
  TGACAAAGTCATCCTCCGTCTACCAGAGCGTGCACTTGTGATCCTAAAATAAGCTTCATC
  TCCGGGCTGTGCCCCTTGGGGTGGAAGGGGCAGGATTCTGCAGCTGCTTTTGCATTTCTC
  TTCCTAAATTTCATTGTGTTGATTTCTTTCCTTCCCAATAGGTGATCTTAATTACTTTCA
  GAATATTTTCAAAATAGATATATTTTTAAAATCCTTANNAAAAAAAAAAAANAAAAAAAA
  AAAAAANNAAAAAAAAAAAAAACCTCGACTCTAGATTGCGGCCGCGGTCATAGCTGTTTC
  CTGAACAGATCCCGGGTGGCATCCCTGTGACCCCTCCCCAGTGCCTCTCCTGGCCCTGNA
  AGTTGCCACTCCAGTGCCCACCAGCCTTGTCCTAATAAAATTAAGTTGCATCATTTTGTC
  TGACTAGGTGTCCTTCTATAATATATGGGGTGGAGGNGGGTTGNAATGGACCAAGGGCCA
  GTTTGGAAAACACCTGTAGGGCCGCGGGGCCTATGGGAACCAGCTGAAT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000100 unedited
  GGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT
  TTTTTTTAAGGATTTTAAAAATATATTTATTTTGAAAATATTCTGAAAGTAATTAAAATC
  ACCTTTTGGGAAGGAAAGAAATCAACCCAATGAAATTTAGGAAAAAAAATGCAAAAGCAG
  CTGCAAAATCCTGCCCCTTCCACCCCAAGGGGCACAGCCCGGAAATGAAGCTTATTTTAG
  GATCACAAGTGCCCCCTCTGGTAAACGGAGGATGACTTTGTCAGTCTTCTGGCTGAAGGG
  CCTTGTCCAAAGTCAGGATCAAAAATAGGGCAGCTCATCATGCTTGGCTTTGTTGGTCTG
  GTAATTAAATAAGGGCAAGGGCTTGTTTTCATGAGGGAAAAATTGGAACACTCGCAGGTG
  TACAAAGTCCTCGTCGCCGACGTGCACCTTGATGAAGTAGTTTGTCCCCGCGACCACCTG
  GCTCTTGAATGACACGGCCTTAAACACAGGGAACTTCTTGTTTTCTTTCTCTTCAAGCTG
  GGACCTCACCTGGTCGGCGATGTGCTGGGTCTCGGCGGTGGCCGGCTGCGTGGCGGAGGG
  CCCCCCGCACATCATCTTGGCGGCGACCCTCGTGCCGAATTCGGCACGAGGCCCTCGTGC
  CGAATTCGCGGCCGCCCTATAGTGAGTCGTATACAAAATTCTGACGGTTCACTAAACGAG
  CTCTGCTTATATAGACCTCCCACCGTACACGCCTACCGNCCATTTGCGTCACGGGGCGGN
  GTTATTACGACTTTTGAAAAGTCCGTTGATTTTGTGCCAAACAACTCCNATGACGTCATG
  GGGTGGAAACTGAAATCCCGGATCAACCGTATCCGCCATTGTGTCTGCAAACGATCACTG
  TATAGCAGACTATACCAAGTCTGCANTAGAAATCGTAGGCATTCTGGCTATGCAGCGGCC
  TTCC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000100
• Insert Size: 640 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000100.2, NP_000091.1
• RefSeq Size: 674 bp
• RefSeq ORF: 297 bp
• Locus ID: 1476
• UniProt ID: P04080
• Cytogenetics: 21q22.3
• Domains: CY
• Gene Summary: The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. There are three inhibitory families in the superfamily, including the type 1 cystatins (stefins), type 2 cystatins and kininogens. This gene encodes a stefin that functions as an intracellular thiol protease inhibitor. The protein is able to form a dimer stabilized by noncovalent forces, inhibiting papain and cathepsins l, h and b. The protein is thought to play a role in protecting against the proteases leaking from lysosomes. Evidence indicates that mutations in this gene are responsible for the primary defects in patients with progressive myoclonic epilepsy (EPM1). One type of mutation responsible for EPM1 is the expansion in the promoter region of this gene of a CCCCGCCCCGCG repeat from 2-3 copies to 30-78 copies. [provided by RefSeq, Jul 2016]