Chloride Channel 5 (CLCN5) (NM_000084) Human Untagged Clone

SKU SC100001

CLCN5 (untagged)-Human chloride channel 5 (CLCN5), transcript variant 3

Product Data

• Type: Human Untagged Clone
• Target Symbol: Chloride Channel 5
• Synonyms: ClC-5; CLC5; CLCK2; DENT1; DENTS; hCIC-K2; NPHL1; NPHL2; XLRH; XRN
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_000084 edited; GGGCGGCCGCGAATTCGGCACGAGGGGCACGAGGAAGACCTGATGGACATTCCAGCAACC; GCTATGGATTTCTCCATGAGAGATGATGTTCCTCCCTTAGACCGAGAAGTAGGAGAGGAC; AAGTCGTACAATGGTGGAGGAATAGGTTCTTCAAATAGGATCATGGACTTCTTGGAGGAG; CCAATCCCTGGTGTAGGGACCTATGATGATTTCAATACAATTGATTGGGTGAGAGAGAAG; TCTCGAGACCGGGATAGGCACCGAGAGATTACCAATAAAAGCAAAGAGTCAACATGGGCC; TTAATTCACAGTGTGAGTGATGCTTTTTCCGGCTGGTTGTTGATGCTCCTTATTGGGCTT; TTATCAGGTTCGTTAGCTGGTTTGATAGACATCTCTGCTCATTGGATGACAGACTTAAAA; GAAGGTATATGCACAGGGGGATTCTGGTTTAACCATGAACATTGTTGCTGGAACTCTGAG; CATGTCACCTTTGAAGAGAGAGACAAATGTCCAGAGTGGAATAGTTGGTCCCAGCTTATC; ATCAGCACAGATGAGGGAGCCTTTGCCTACATAGTCAATTATTTCATGTACGTCCTCTGG; GCTCTCCTATTTGCCTTCCTTGCCGTATCTCTTGTCAAGGTGTTTGCGCCTTATGCCTGT; GGCTCTGGAATCCCTGAGATAAAAACTATCTTGAGTGGTTTCATTATTAGGGGCTATTTG; GGTAAGTGGACTCTGGTTATCAAAACCATCACCTTGGTGCTGGCAGTGTCGTCTGGCTTG; AGCCTGGGCAAAGAGGGCCCTCTAGTGCACGTGGCTTGCTGCTGTGGGAACATCCTGTGC; CACTGCTTCAACAAATACAGGAAGAATGAAGCCAAGCGCAGAGAGGTCTTGTCGGCTGCA; GCAGCAGCTGGTGTATCTGTAGCCTTTGGAGCACCTATAGGTGGAGTATTATTCAGCCTT; GAAGAGGTCAGCTACTATTTTCCCCTCAAAACATTGTGGCGTTCATTCTTTGCTGCCTTG; GTGGCAGCATTCACTCTACGCTCCATCAATCCATTTGGGAACAGCCGCCTGGTACTATTT; TATGTGGAGTTTCACACCCCATGGCATCTCTTTGAGCTCGTGCCATTCATTCTGCTGGGC; ATATTTGGTGGTCTGTGGGGAGCACTGTTTATCCGCACAAACATTGCCTGGTGTCGGAAG; CGAAAGACCACCCAGTTGGGCAAGTATCCTGTTATAGAGGTACTCGTCGTGACAGCCATC; ACTGCCATCCTGGCTTTCCCCAATGAATACACTCGGATGAGCACAAGTGAGCTCATTTCT; GAGCTGTTTAATGACTGTGGCCTTCTGGACTCCTCCAAGCTCTGTGATTATGAGAACCGT; TTCAACACAAGCAAAGGGGGTGAACTGCCTGACAGACCGGCTGGCGTGGGAGTCTACAGT; GCAATGTGGCAGCTGGCTTTAACACTCATACTGAAAATTGTCATTACTATATTCACCTTT; GGCATGAAGATCCCTTCTGGCCTCTTTATCCCTAGCATGGCTGTTGGTGCTATAGCAGGT; CGACTTCTAGGAGTAGGAATGGAACAGCTGGCTTATTACCACCAGGAATGGACCGTCTTC; AATAGCTGGTGTAGTCAGGGAGCTGATTGCATCACCCCCGGCCTTTATGCAATGGTTGGG; GCTGCAGCCTGCTTAGGTGGGGTGACTCGGATGACTGTTTCTCTTGTTGTCATAATGTTT; GAACTGACTGGTGGCTTAGAATACATCGTGCCTCTGATGGCTGCAGCCATGACAAGCAAG; TGGGTGGCAGATGCTCTTGGGCGGGAGGGCATCTATGATGCCCACATCCGTCTCAATGGA; TACCCCTTTCTTGAAGCCAAAGAAGAGTTTGCTCATAAGACCCTGGCAATGGATGTGATG; AAACCCCGGAGAAATGATCCTTTGTTGACTGTCCTTACTCAGGACAGTATGACTGTGGAA; GATGTAGAGACCATAATCAGTGAAACCACTTACAGTGGCTTCCCAGTGGTGGTATCCCGG; GAGTCCCAAAGACTTGTGGGCTTTGTCCTCCGAAGAGATCTCATTATTTCAATTGAAAAT; GCTCGAAAGAAACAGGATGGGGTTGTTAGCACTTCCATCATTTATTTCACGGAGCATTCT; CCTCCATTGCCACCATACACTCCACCCACTCTAAAGCTTCGGAACATCCTCGATCTCAGC; CCCTTCACTGTGACTGACCTTACACCCATGGAGATCGTAGTGGATATTTTCCGAAAGCTG; GGACTGCGGCAGTGCCTGGTTACACACAACGGGCGATTGCTTGGAATCATTACCAAAAAG; GATGTGTTAAAGCATATAGCACAGATGGCGAACCAAGATCCTGATTCCATTCTCTTCAAC; TAGAATCATAGAGTTCTGGATGTAAAGCGGGAAGGACATTACAGACCATGGATATGTTGT; ATTAACTGGGTACCCAAAACACATTTTCCATATTTGGATGGTGAAGTCACATTAGTGTGT; TGTCTCTTTCCTACAAGTTAACCAGTTGCACTACATAATCTCTGGAAATTAATTTTCTCT; TTAGGAGAAATTATAGTTAGGCTTCCATGATGTTACATTAGGAAGATATCATGAAAGAAT; AAATAAGATTGCTATGGTTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA; AAAAAAAAAAAAAAAAAAAAAAAAA;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000084 unedited; TTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGGCACGAGGAAGACC; TGATGGACATTCCAGCAACCGCTATGGATTTCTCCATGAGAGATGATGTTCCTCCCTTAG; ACCGAGAAGTAGGAGAGGACAAGTCGTACAATGGTGGAGGAATAGGTTCTTCAAATAGGA; TCATGGACTTCTTGGAGGAGCCAATCCCTGGTGTAGGGACCTATGATGATTTCAATACAA; TTGATTGGGTGAGAGAGAAGTCTCGAGACCGGGATAGGCACCGAGAGATTACCAATAAAA; GCAAAGAGTCAACATGGGCCTTAATTCACAGTGTGAGTGATGCTTTTTCCGGCTGGTTGT; TGATGCTCCTTATTGGGCTTTTATCAGGTTCGTTAGCTGGTTTGATAGACATCTCTGCTC; ATTGGATGACAGACTTAAAAGAAGGTATATGCACAGGGGGATTCTGGTTTAACCATGAAC; ATTGTTGCTGGAACTCTGAGCATGTCACCTTTGAAGAGAGAGACAAATGTCCAGAGTGGA; ATAGTTGGTCCCAGCTTATCATCAGCACAGATGAGGGAGCCTTTGCCTACATAGTCAATT; ATTTCATGTACGTCCTCTGGGCTCTCCTATTTGCCTTCCTTGCCGTATCTCTTGTCAAGG; TGTTTGCGCCTTATGCCTGTGGCTCTGGAATCCCTGAGAAAAAACTATCTGAGTGGTTTA; TTATTAGGNGCTTTTGGGTAAGTGGACTCTGGTATCAAACCATCACCTGGGTGCTGCAGT; GTCGTCTGGCTTGAGCCTGGCAAAGAGGGCCTCTANTGCACGTGGCTTGCTGCTGTGGGA; ACATNCTGTGCCACTGCTTCACAATACAGGAGGATGAAGCAA;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000084 unedited; GCCGCGGCCGCAATCTAGAGTCGAGTNTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT; TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAACCATAACAATCTTATTTATTTTTTCA; TGAAATCTTCCTAAGGGAACATCTTGGAACCCTAACTTTAATTTTTCCTAAAAAAAAAAT; TAATTTCCAAAAATTTTGTAGGGCAACGGGTTAACTTGGTGGGGAAAAGAGAAACACCCT; AATGTGACTTCCCCATCCAAATTTGGAAAATGTGTTTTGGGTACCCAGTTAATACAACAT; ATCCCTGGTCTGTAATGGCCTTCCCGTTTACATCCAGAACTTTTTGATTCTAGTTGAAAA; AAATGGAATCAGGATCTTTGGTCCGCCATCTGGGCTAATAGGCTTAAACACATCCTTTTT; GATAATGATTCCAACCAATTCCCCGTTGTGTTGTACCCAGCCCCTGCCGGAATCCCAGTT; TTTGGAAAAATTCCCCCTCCAATCCCCATGGGGGTAAGGCCCACCCCCGGGGAAGGGGGC; TGAAATCGAGGATGTTCCACAAACCTTTAAAAGGGGTGGGATTGTTGGGGGCAATGAAGG; AAATCCCCCCTAAAAAAATGATGGAAGGCTACCACCCCTTCTGGTTTTTTCTGCCTTTTT; AATTGAAATAAGGAATCTTTTGGGGGGAAAAACCCCAAATTTTTGGGAACTCCGGGGATA; CCCCCCTTGGAAACCCCGGAGAGGGTNTTCCCGATTATGGGTTTTAATTTTCCAAAATTT; TCTGTCTTGGAAAGGAAACCCAAAAGGATTTTTTTCCGGGTTTATCACCTTCTTGCCCGG; GGTTTTAAAAAACTTTTTTTGTTTAAAAAGGGGTTTCCTTGAACGGGTGGGGCTATTAAT; CCCCCCCCCAAAACTTTCCCCCCTTTTTTTTTGGGTTGGCCTATAGGCCCTTTTTTTTAA; CCCCGTGTTTAAATTTTAACAAAAAAAATTTTTGTTCCCCCTTAAGGGGGGCCCACATTT; TAG;
• Restriction Sites: NotI-NotI
• ACCN: NM_000084
• Insert Size: 2241 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_000084.1, NP_000075.1
• RefSeq Size: 3173 bp
• RefSeq ORF: 2241 bp
• Locus ID: 1184
• UniProt ID: P51795
• Cytogenetics: Xp11.23
• Domains: CBS, voltage_CLC
• Protein Families: Druggable Genome, Ion Channels: Other, Transmembrane
• Summary: This gene encodes a member of the ClC family of chloride ion channels and ion transporters. The encoded protein is primarily localized to endosomal membranes and may function to facilitate albumin uptake by the renal proximal tubule. Mutations in this gene have been found in Dent disease and renal tubular disorders complicated by nephrolithiasis. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jan 2013]; Transcript Variant: This variant (3, also known as type 3) lacks several 5' exons but has an alternate 5' exon, and it thus differs in the 5' UTR and uses a downstream start codon, compared to variant 1. The resulting isoform (b) has a shorter N-terminus, compared to isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.