Ghdc (NM_001191651) Rat Tagged ORF Clone

CAT#: RR216855

Ghdc (myc-DDK-tagged) - Rat GH3 domain containing (Ghdc)

Product Images

Circular map for RR216855

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ghdc
• Synonyms: RGD1308881
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR216855 representing NM_001191651
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCTGTGCTGTGGCTGCTGTTTCTGCTGCCGCTCCTGCCCCTGCTGGCCATGCTCTGCCAGCAGAGAT
  CTCCGGGTGCCAGACCATGCTGGCTGATCAGTCTCCAGCACCGGGTGGCTTGCGTGGTGCTGAGCTGGGC
  AGCCGCCTGGCAGCGGCGGAAACTAGAACAGAGCACACTGAACGTGAGCCAGAGCCAGCAGCAGGCCCTC
  ATGGGGTGTCTGAAGGAAGCCCAGGGTTCCTGCTGTCTCCCCAGGGAGAACACAGACATGACCACCTTCC
  GGAATCTTCCACTGACAAAAACCAGCCACACCCAGCAGAAAGAAAGTGAAGAGAAGCTCCTGCCCCCTAC
  CTTACCCCAGTACCATGGAGATGCCTCTTTGCAGGCCACCCTGCTGGGTCTAATGACCTTAAACAAGGCC
  TATCCAGAAGTGCTGGCTCCAGGAAGTACTGCCTGTGTGACCCCTACATCCCCATGGCCCTATTCTGTCC
  CCTGGCTCGGGCATGCCCTGGGCCGGGTAAGTCCCATTGGAGCCAAGGATGCCCGGACCCTGCTGCTGGA
  GGCGTTGATATCCCCGGGGCTGAGGGTACTTGAGGCAAGGACTGCAGTGGAGCTCCTGGATGTCTTTGTG
  GGCTTGGAGGCTGATGGTGAAGAACTGGCTGAGGTCATAGCATCTGGGAGCCTAGGGAAGCTCCCCAGAC
  GAGCAGCTGAGCTGCAGGAGGCCCTAGAGCAGGGACCCCGAGGATTGGCCCTCCGGCTCTGGCCAAAGCT
  GCAGGTGGTGGTGACTCTGGACGCAGGAGGACAGGCAGAAGCTGTGGCTGCCCTTAGGGTCCTGTGGTGC
  CAAGGGCTAGCCTTCTTCTCTCCTGCTTATGCTGCCTCTGGAGGGGTGATGGCCCTAAACCTGTGGCCAG
  AGCAACCCCAAGGATCCTATCTTCTGTCCCCTGGAGTCCCCTTTATTGAGCTGCTTCCAATCAAGGAAGG
  AACCCAAGAGGAGGCAGCCTCCACTCTCCTTCTTACTGATGTCCGAAGGGAGGAAAAGTATGAGTTGGTC
  CTGACTGACAGCACCAGCCTGACCAGGTGCCGCCTGGGTGACGTGGTACAGGTGATTGGCACCTACAATC
  AGTGTCCTGTGGTCAGGTTCACCTGCAGGCTGGGACAGACCCTGAGTGTTCGAGGAGAAGTTACTGATGA
  GAATGTATTCTCTGTGGCTTTGGCCCAAGCAGTGGGGCAGTGGCCAGGGGCCAAGCTGTTGGACCATGTC
  TGTGTGGAGAGCCACATTCTGGACTCCTATGAGGGATCCGCCCCACACTACGAAGTGTTTGTGGAGCTGA
  GGGGCTTGAGGAATCTGTCGGAGGAAAATCGAGACAAGCTTGACCACTGCCTTCAGGAAGCCTCCCCTCG
  GTACAAGTCCTTGCGGTTCTGGGGCAGCGTGGGTCCTGCCAAAGTCCATCTGGTGAGGCCAGGAAGCTTC
  AGAGTACTTCGGGAAGCACTAGCAGCCTGCCCCTCCTCTTCCTGTCCTGAAATGCCTCGGGTCCTCAGGC
  TCAGGCACCTGGCCCAGCTCCTGCAGAAGAGGGTGATACCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR216855 representing NM_001191651
  Red=Cloning site Green=Tags(s)
  
  MPVLWLLFLLPLLPLLAMLCQQRSPGARPCWLISLQHRVACVVLSWAAAWQRRKLEQSTLNVSQSQQQAL
  MGCLKEAQGSCCLPRENTDMTTFRNLPLTKTSHTQQKESEEKLLPPTLPQYHGDASLQATLLGLMTLNKA
  YPEVLAPGSTACVTPTSPWPYSVPWLGHALGRVSPIGAKDARTLLLEALISPGLRVLEARTAVELLDVFV
  GLEADGEELAEVIASGSLGKLPRRAAELQEALEQGPRGLALRLWPKLQVVVTLDAGGQAEAVAALRVLWC
  QGLAFFSPAYAASGGVMALNLWPEQPQGSYLLSPGVPFIELLPIKEGTQEEAASTLLLTDVRREEKYELV
  LTDSTSLTRCRLGDVVQVIGTYNQCPVVRFTCRLGQTLSVRGEVTDENVFSVALAQAVGQWPGAKLLDHV
  CVESHILDSYEGSAPHYEVFVELRGLRNLSEENRDKLDHCLQEASPRYKSLRFWGSVGPAKVHLVRPGSF
  RVLREALAACPSSSCPEMPRVLRLRHLAQLLQKRVIP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001191651
• ORF Size: 1581 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001191651.1, NP_001178580.1
• RefSeq Size: 2160 bp
• RefSeq ORF: 1584 bp
• Locus ID: 303542
• Cytogenetics: 10q31
• MW: 57.9 kDa