Angpt1 (NM_053546) Rat Tagged ORF Clone

CAT#: RR212791

  • TrueORF®

Angpt1 (Myc-DDK-tagged ORF) - Rat angiopoietin 1 (Angpt1), (10 ug)



  "NM_053546" in other vectors (3)

Reconstitution Protocol

USD 503.00

4 Weeks*

Size
    • 10 ug

Product Images

Frequently bought together (4)
DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
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pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

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DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

USD 160.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

USD 59.00

Other products for "Angpt1"

Specifications

Product Data
Type Rat Tagged ORF Clone
Tag Myc-DDK
Symbol Angpt1
Synonyms Agpt; Agpt1; Ang-1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RR212791 representing NM_053546
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGACAGTTTTCCTTTCCTTTGCATTCTTCGCTGCCATTCTGACTCACATAGGGTGCAGCAACCAGCGCC
GGAGTCCAGAAAACGGAGGGAGAAGATATAACCGAATTCAACATGGGCAATGTGCCTACACTTTCATTCT
TCCAGAACACGACGGGAACTGCCGTGAGAGTGCGACAGAGCAGTACAACACCAACGCTCTGCAAAGGGAT
GCTCCACACGTGGAGACGGATTTCTCTTCCCAGAAACTTCAGCATCTGGAGCATGTGATGGAAAATTATA
CTCAGTGGCTGCAAAAACTTGAGAATTACATTGTGGAAAATATGAAGTCGGAGATGGCCCAGATACAACA
GAATGCGGTTCAAAACCACACGGCCACCATGCTGGAGATAGGAACCAGCCTCTTGTCTCAGACTGCAGAG
CAGACCCGAAAGCTCACAGATGTGGAGACCCAGGTACTAAATCAAACATCCCGTCTTGAAATCCAACTGC
TGGAGAATTCATTATCAACATACGAGCTAGAGAAACAGCTTCTCCAACAGACAAATGAAATTCTGAAGAT
TCAGGAAAAAAACAGTTTATTAGAGCATAAAATCCTAGAAATGGAGGGAAAACACAAGGAAGAGCTGGAC
ACCTTGAAGGAGGAGAAAGAAAACCTTCAAGGCTTGGTTACTCGTCAGACATTCATCATCCAAGAATTGG
AGAAGCAACTTAGCAGAGCTACCAGCAACAACAGTGTTCTGCAGAAGCAACAACTGGAGCTCATGGACAC
AGTCCATAACCTTGTCAGCCTTTGCACAAAAGAAGTTTTGCTAAAGGGAGGAAAAAGAGAAGAAGAGAAA
CCATTTCGAGACTGTGCAGATGTATATCAAGCTGGTTTTAATAAGAGTGGAATCTACACTATTTATTTTA
ATAATATGCCAGAACCCAAAAAGGTATTTTGCAATATGGATGTGAATGAAGGAGGATGGACAGTAATACA
ACACCGTGAGGATGGAAGCCTAGATTTCCAGAGGGGCTGGAAGGAGTATAAAATGGGTTTTGGGAATCCC
TCTGGTGAATATTGGCTTGGGAACGAGTTCATTTTTGCAATAACCAGTCAGAGGCAGTACATGCTGAGGA
TCGAGCTGATGGACTGGGAAGGGAACCGAGCCTACTCACAGTACGACAGATTCCACATAGGAAACCAGAA
GCAGAACTACAGGTTATATTTAAAGGGTCACACGGGGACAGCAGGCAAACAGAGCAGCTTGATCTTACAT
GGTGCTGATTTCAGCACAAAGGACGCTGATAACGACAACTGTATGTGCAAATGCGCCCTTATGCTAACAG
GAGGTTGGTGGTTTGATGCCTGTGGCCCTTCCAATCTAAACGGAATGTTCTACACTGCAGGGCAAAACCA
TGGAAAACTGAATGGGATAAAGTGGCACTACTTCAAAGGACCCAGTTACTCCTTACGTTCCACAACCATG
ATGATCCGGCCCTTGGACTTC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RR212791 representing NM_053546
Red=Cloning site Green=Tags(s)

MTVFLSFAFFAAILTHIGCSNQRRSPENGGRRYNRIQHGQCAYTFILPEHDGNCRESATEQYNTNALQRD
APHVETDFSSQKLQHLEHVMENYTQWLQKLENYIVENMKSEMAQIQQNAVQNHTATMLEIGTSLLSQTAE
QTRKLTDVETQVLNQTSRLEIQLLENSLSTYELEKQLLQQTNEILKIQEKNSLLEHKILEMEGKHKEELD
TLKEEKENLQGLVTRQTFIIQELEKQLSRATSNNSVLQKQQLELMDTVHNLVSLCTKEVLLKGGKREEEK
PFRDCADVYQAGFNKSGIYTIYFNNMPEPKKVFCNMDVNEGGWTVIQHREDGSLDFQRGWKEYKMGFGNP
SGEYWLGNEFIFAITSQRQYMLRIELMDWEGNRAYSQYDRFHIGNQKQNYRLYLKGHTGTAGKQSSLILH
GADFSTKDADNDNCMCKCALMLTGGWWFDACGPSNLNGMFYTAGQNHGKLNGIKWHYFKGPSYSLRSTTM
MIRPLDF

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_053546
ORF Size 1491 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_053546.1, NP_445998.1
RefSeq Size 1891 bp
RefSeq ORF 1494 bp
Locus ID 89807
UniProt ID O35460
Cytogenetics 7q31
MW 57.5 kDa
Gene Summary modulates endothelial cell survival and involved in the activation of endothelial cell-specific Tie-2 receptors [RGD, Feb 2006]

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.