Foxo3 (NM_001106395) Rat Tagged ORF Clone

CAT#: RR210446

Foxo3 (Myc-DDK-tagged ORF) - Rat forkhead box O3 (Foxo3), (10 ug)

Product Images

Circular map for RR210446

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Foxo3
• Synonyms: Fkhrl1; Foxo3a
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR210446 representing NM_001106395
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAGAGGCACCAGCCTCCCCGGTCCCGCTCTCTCCGCTCGAAGTGGAGCTGGACCCAGAGTTCGAGC
  CACAGAGTCGGCCACGCTCCTGTACGTGGCCCCTGCAGAGGCCGGAGCTGCAGGCGAGCCCGGCCAAGCC
  CTCGGGGGAGACGGCGGCAGACTCCATGATCCCCGAGGAGGACGACGATGAAGACGACGAGGACAGCGGC
  GGCCGAGGCAGCTCGGCCATGGTGATCGGTGGCGGCGTGAGCAGTGCGCTGGGCTCCGGGCTGCTCCTCG
  AAGATTCGGCCAGGCTGCTGTCTCCCGGAGGGCAGGACCTCGGGACGGGGCCAGCGTCCTCCGCAGGCGC
  GCTGAGTGGGGGCACGCAGACGCAGCTGCAGCCTCAGCAGCCACTGCCACCGCCGCAGCCGGGGGCGGCT
  GGGGGCTCTGGGCAACCGAGGAAATGCTCCTCGCGGCGGAATGCCTGGGGGAACCTGTCCTACGCTGACC
  TGATCACCCGCGCCATCGAGAGCTCCCCGGACAAACGGCTCACTTTGTCCCAGATCTACGAGTGGATGGT
  GCGCTGTGTGCCCTACTTCAAGGATAAGGGCGACAGCAACAGCTCTGCGGGCTGGAAGAACTCCATCCGC
  CACAACCTGTCACTGCACAGCCGTTTCATGCGGGTTCAGAACGAAGGGACTGGCAAGAGCTCTTGGTGGA
  TCATCAACCCCGATGGGGGAAAGAGTGGGAAGGCTCCCCGGCGGCGGGCCGTCTCCATGGACAACAGCAA
  CAAGTACACCAAGAGCCGAGGCCGGGCAGCCAAGAAGAAGGCGGCCCTGCAGGCTGCCCCAGAGTCGGCA
  GACGACAGCCCTTCCCAACTCTCCAAGTGGCCGGGCAGCCCCACATCCCGCAGCAGCGACGAGCTGGACG
  CCTGGACCGACTTCCGCTCGCGCACCAATTCCAACGCCAGCACCGTGAGCGGCCGCCTGTCACCCATCTT
  GGCGAGCACGGAGCTGGATGACGTCCAGGACGATGACGGGCCCCTCTCCCCCATGCTCTACAGCAGCTCT
  GCCAGCCTGTCGCCTTCCGTAAGCAAGCCGTGTACCGTGGAGCTGCCGCGGCTGACTGACATGGCGGGCA
  CCATGAATCTGAACGATGGGCTGGCCGAGAACCTCATGGATGACCTGCTAGATAACATCGCGCTCCCGCC
  CTCCCAGGCGTCGCCTCCTGGCGGGCTTATGCAGCGGAGCTCCAGCTTCCCGTACACCGCCAAGAGCTCC
  GGCCTGGGCTCCCCAACCAGCTCCTTTAACAGTACCGTGTTCGGACCTGCGTCACTCAACTCCCTGCGCC
  AGTCGCCCATGCAGACCATCCAGGAGAACAGACCAGCCACCTTTTCTTCCGTGTCGCACTACGGCAACCA
  GACACTCCAAGACCTGCTCACTTCGGACTCGCTCAGCCACAGCGATGTCATGATGACCCAGTCAGACCCC
  TTGATGTCTCAGGCTAGCACCGCTGTGTCCGCCCAGAACGCCCGCCGGAACGTGATGCTTCGCAACGACC
  CAATGATGTCCTTTGCCGCCCAGCCTACCCAGGGGAGTTTGGTCAATCAGAACTTGCTCCACCACCAGCA
  CCAAACCCAGGGCGCTCTTGGTGGCAGCCGTGCCTTGTCAAATTCTGTCAGCAACATGGGCTTGAGTGAC
  TCCAGCAGCCTCGGCTCAGCCAAACACCAGCAGCAGTCTCCCGTCAGCCAGTCTATGCAAACCCTCTCGG
  ACTCTCTCTCAGGCTCCTCACTGTATTCAGCCAGTGCAAACCTTCCCGTCATGGGTCACGACAAGTTCCC
  CAGTGACTTGGACCTGGACATGTTCAATGGGAGCTTGGAGTGTGACATGGAGTCCATCATCCGTAGCGAA
  CTCATGGATGCCGATGGGTTGGATTTTAACTTTGACTCCCTCATCTCCACACAGAACGTTGTTGGTTTGA
  ACGTGGGGAACTTCACTGGTGCTAAGCAGGCCTCATCTCAAAGCTGGGTGCCAGGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR210446 representing NM_001106395
  Red=Cloning site Green=Tags(s)
  
  MAEAPASPVPLSPLEVELDPEFEPQSRPRSCTWPLQRPELQASPAKPSGETAADSMIPEEDDDEDDEDSG
  GRGSSAMVIGGGVSSALGSGLLLEDSARLLSPGGQDLGTGPASSAGALSGGTQTQLQPQQPLPPPQPGAA
  GGSGQPRKCSSRRNAWGNLSYADLITRAIESSPDKRLTLSQIYEWMVRCVPYFKDKGDSNSSAGWKNSIR
  HNLSLHSRFMRVQNEGTGKSSWWIINPDGGKSGKAPRRRAVSMDNSNKYTKSRGRAAKKKAALQAAPESA
  DDSPSQLSKWPGSPTSRSSDELDAWTDFRSRTNSNASTVSGRLSPILASTELDDVQDDDGPLSPMLYSSS
  ASLSPSVSKPCTVELPRLTDMAGTMNLNDGLAENLMDDLLDNIALPPSQASPPGGLMQRSSSFPYTAKSS
  GLGSPTSSFNSTVFGPASLNSLRQSPMQTIQENRPATFSSVSHYGNQTLQDLLTSDSLSHSDVMMTQSDP
  LMSQASTAVSAQNARRNVMLRNDPMMSFAAQPTQGSLVNQNLLHHQHQTQGALGGSRALSNSVSNMGLSD
  SSSLGSAKHQQQSPVSQSMQTLSDSLSGSSLYSASANLPVMGHDKFPSDLDLDMFNGSLECDMESIIRSE
  LMDADGLDFNFDSLISTQNVVGLNVGNFTGAKQASSQSWVPG
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001106395
• ORF Size: 2016 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001106395.1, NP_001099865.1
• RefSeq Size: 2885 bp
• RefSeq ORF: 2019 bp
• Locus ID: 294515
• Cytogenetics: 20q12-q13
• MW: 71.2 kDa