Agps (NM_053350) Rat Tagged ORF Clone

CAT#: RR210194

Agps (Myc-DDK-tagged ORF) - Rat alkylglycerone phosphate synthase (Agps), (10 ug)

Product Images

Circular map for RR210194

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Agps
• Synonyms: Adap-s; Adps; Ads
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR210194 representing NM_053350
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTCGCGCGGGGCGCCGCTCTGAGTGACCTTTCACCCGCGCCCAGCGGCCTCGGGCGGCAGCACAAGG
  CGGAAGCCATGGCGGAGGCGGCGGGAGAGGCGGGCGCGAGCGAGCGGGACCCAGACGCGGTCCGCGCGCG
  TCGGCGGCTGCGGGTTCTGTCGGGCCATCTGCTGGGCCGGCCCCAGGAGGCTCCGAGTACCAATGAGTGC
  AAAGCGCGGAGAGCCGCGTCGGCGGCCGGGGCGTCGCCCGCTGCTTCTCCCGCCGCGCCGGAGTCGGGCA
  CCATCCCCAAGAAGCGGCAAGAACTTATGAAATGGAATGGATGGGGCTATAATGATTCCAAGTTCTTACT
  GAATAAGAAGGGCCAGGTTGAGCTGACTGGGAAAAGGTACCCTCTTAGTGGCTTGGCTTTACCAACTTTG
  AAAGACTGGATCCAAAACACCCTTGGAGTAAATCTGGAGCATAAAACTACCTCTAAACCATCCATAAATC
  CTAGTGAAGCACCTCCTTCTATTGTGAATGAAGATTTCCTTCAGGAACTTAAAGAAGCTCATATTTCCTA
  CTCACAAGACGCAGATGATCGTGTGTTTAGAGCTCACGGTCATTGTCTTCATGAGATATTTTTGCTCAGA
  GAAGGGATGTTTGAGCGGATTCCTGATATAGTTGTATGGCCAACATGTCATGATGATGTAGTTAAGATTG
  TGAATCTAGCGTGCAAATATAACCTTTGTATCATACCAATTGGTGGGGGAACGAGTGTATCCTATGGCCT
  GATGTGCCCTGCAGATGAGACAAGAACAATAATTTCTTTGGATACTTCACAAATGAACCGGATTCTCTGG
  GTTGATGAGAACAATCTGACAGCTCACGTAGAGGCTGGGATAACAGGACAAGATTTGGAAAGACAGCTTA
  AAGAAAGTGGTTATTGTACAGGTCATGAACCGGACTCCCTAGAATTCAGCATCGTGGGAGGATGGATATC
  TACTCGAGCATCAGGCATGAAGAAGAATGTCTACGGCAATATTGAGGACCTGGTGGTTCACATGAAAATG
  GTAACACCTAGAGGTGTAATAGAGAAAAGCTCCCAAGGCCCTCGGATGTCAACGGGGCCTGATATCCATC
  ACTTCATCATGGGCTCTGAAGGAACTCTTGGTGTAATAACAGAAGCTACAATAAAAATCAGACCAACTCC
  TGAATACCAAAAGTATGGTTCAGTGGCTTTCCCTAATTTTGAACAAGGTGTAGCCTGTTTAAGAGAAATT
  GCAAAGCAGAGATGTGCTCCTGCATCTATCCGCCTCATGGACAACCAGCAGTTTCAGTTTGGGCATGCTC
  TTAAACCTCAGGTCTCCTCTATTTTTACGTCATTTTTGGATGGATTCAAAAAGTTTTATATTACAAAGTT
  TAAAGGATTTGATCCAAATCAAATAAGCGTAGCCACATTACTGTTCGAGGGAGACCGTGAGAAGGTTCTT
  CAGCATGAAAAACAAGTGTATGACATCGCAGCAAAATTTGGTGGTCTGGCTGCTGGAGAAGATAACGGGC
  AGAGGGGTTATTTGCTGACTTATGTCATTGCATACATGCGTGATTTGGGTTTGGAATACTATGTAGTAGG
  AGAATCTTTTGAGACTTCTGCTCCTTGGGACAGGGTTATAGATCTGTGTAGAAATGTAAAAGAAAGGATA
  AGAAGGGAATGCAAAGAAAGGGGAGTTCAGTTTGCGCCTCTTTCTACGTGCAGGGTGACGCAGACTTACG
  ATGCAGGTGCATGTATCTACTTCTATTTTGCCTTTAACTACAGGGGAATTAGTGACCCACTGACTGTGTT
  TGAACAGACTGAGGCAGCTGCTAGAGATGAAATCCTTGCCAATGGAGGGAGCCTGTCACATCACCATGGA
  GTGGGCAAGTTACGAAAGCAGTGGCTAAAGGAGAGCATCTCTGATGTCGGCTTTGGGATGCTAAAGTCTG
  TCAAAGACTATGTGGACCCCAGTAACATCTTTGGAAACAGAAACCTTTTA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR210194 representing NM_053350
  Red=Cloning site Green=Tags(s)
  
  MVARGAALSDLSPAPSGLGRQHKAEAMAEAAGEAGASERDPDAVRARRRLRVLSGHLLGRPQEAPSTNEC
  KARRAASAAGASPAASPAAPESGTIPKKRQELMKWNGWGYNDSKFLLNKKGQVELTGKRYPLSGLALPTL
  KDWIQNTLGVNLEHKTTSKPSINPSEAPPSIVNEDFLQELKEAHISYSQDADDRVFRAHGHCLHEIFLLR
  EGMFERIPDIVVWPTCHDDVVKIVNLACKYNLCIIPIGGGTSVSYGLMCPADETRTIISLDTSQMNRILW
  VDENNLTAHVEAGITGQDLERQLKESGYCTGHEPDSLEFSIVGGWISTRASGMKKNVYGNIEDLVVHMKM
  VTPRGVIEKSSQGPRMSTGPDIHHFIMGSEGTLGVITEATIKIRPTPEYQKYGSVAFPNFEQGVACLREI
  AKQRCAPASIRLMDNQQFQFGHALKPQVSSIFTSFLDGFKKFYITKFKGFDPNQISVATLLFEGDREKVL
  QHEKQVYDIAAKFGGLAAGEDNGQRGYLLTYVIAYMRDLGLEYYVVGESFETSAPWDRVIDLCRNVKERI
  RRECKERGVQFAPLSTCRVTQTYDAGACIYFYFAFNYRGISDPLTVFEQTEAAARDEILANGGSLSHHHG
  VGKLRKQWLKESISDVGFGMLKSVKDYVDPSNIFGNRNLL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_053350
• ORF Size: 2010 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_053350.2, NP_445802.2
• RefSeq Size: 2144 bp
• RefSeq ORF: 2013 bp
• Locus ID: 84114
• UniProt ID: Q9EQR2
• Cytogenetics: 3q23
• MW: 74.2 kDa
• Gene Summary: Catalyzes the exchange of an acyl for a long-chain alkyl group and the formation of the ether bond in the biosynthesis of ether phospholipids.[UniProtKB/Swiss-Prot Function]