Mcl1 (NM_021846) Rat Tagged ORF Clone
CAT#: RR209509
- TrueORF®
Mcl1 (Myc-DDK-tagged ORF) - Rat myeloid cell leukemia sequence 1 (Mcl1), (10 ug)
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
AAV Particle: DDK
"NM_021846" in other vectors (3)
Specifications
Product Data | |
Type | Rat Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | Mcl1 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RR209509 representing NM_021846
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTTGGCCTTCGGAGAAACGCGGTAATCGGCTTGAACCTGTACTGCGGCGGCGCTAGCCTCGGCGCGG GCGGCGGCTCTCCGGCCGGGACGCGCCTGGCGGCCGAGGAGGCCAAGGCGCGGCGCGAGGGGGGAGGGGA GGCCGCTCTGCTGCCCGGCGCGCGGGTGGTCGCCCGGCCGCCTCCGGTGGGCGCCGAGGACCCCGACGTC ACCGCGTCGGCAGAGAGGCGGCTGCTCAAGTCGCCCGGCCTCCTCGCCGTGCCGCCTGAGGAGATGGCCG CGTCGGCCGCCGCCATCATGTCTCCCGAGGAGGAGCTGGACGGCTGCGAGCCGGAGGTGCTCAGCAAACG CCCGGCGGTGCTGCCCCTACTGGAGCGCGTGAGCGAGGCGGCTAAGAGCTCCGGAGCTGACGGCTCGCTG CCCTCCACGCCGCCGCCGCCTGAGGAGGAAGACGACGAGCTGTACCGCCAGTCGCTGGAGATCATCTCCC GCTACCTGCGGGAGCAGGCGACGGGCTCCAAGGACGCGAAGCCTCTGGGCGAGGCCGGCGCAGCGGGCCG GAGGGCGCTGGAGACCCTGCGGCGCGTGGGCGACGGCGTGCAGCGCAACCACGAGACGGCCTTCCAGGGC ATGCTTCGGAAACTGGACATTAAAAACGAGGACGATGTTAAATCTTTTTCTCGAGTGATGACCCATGTTT TCAAAGATGGCGTAACAAACTGGGGCAGGATTGTGACTCTTATTTCTTTTGGTGCCTTTGTGGCCAAACA CTTAAAGAGCATAAACCAAGAAAGCTGCATCGAACCTTTAGCAGAAAGTATCACAGACGTTCTTGTAAGG ACGAAGCGGGACTGGCTTGTGAAACAAAGAGGCTGGGATGGGTTTGTGGAGTTCTTCCACGTACAGGACC TAGAAGGCGGCATCAGAAATGTGCTGCTGGCTTTTGCGGGTGTTGCTGGAGTAGGGGCTGGTCTGGCATA TCTAATAAGG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR209509 representing NM_021846
Red=Cloning site Green=Tags(s) MFGLRRNAVIGLNLYCGGASLGAGGGSPAGTRLAAEEAKARREGGGEAALLPGARVVARPPPVGAEDPDV TASAERRLLKSPGLLAVPPEEMAASAAAIMSPEEELDGCEPEVLSKRPAVLPLLERVSEAAKSSGADGSL PSTPPPPEEEDDELYRQSLEIISRYLREQATGSKDAKPLGEAGAAGRRALETLRRVGDGVQRNHETAFQG MLRKLDIKNEDDVKSFSRVMTHVFKDGVTNWGRIVTLISFGAFVAKHLKSINQESCIEPLAESITDVLVR TKRDWLVKQRGWDGFVEFFHVQDLEGGIRNVLLAFAGVAGVGAGLAYLIR TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
Sequencher program is needed, download here. |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_021846 |
ORF Size | 990 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_021846.2, NP_068618.2 |
RefSeq Size | 2130 bp |
RefSeq ORF | 993 bp |
Locus ID | 60430 |
UniProt ID | Q9Z1P3 |
Cytogenetics | 2q34 |
MW | 35.2 kDa |
Gene Summary | ovarian BAD-interacting protein; may inhibit apoptosis [RGD, Feb 2006] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RN209509 | Mcl1 (untagged ORF) - Rat myeloid cell leukemia sequence 1 (Mcl1), (10 ug) |
USD 330.00 |
|
RR209509L3 | Lenti ORF clone of Mcl1 (Myc-DDK-tagged ORF) - Rat myeloid cell leukemia sequence 1 (Mcl1), (10 ug) |
USD 600.00 |
|
RR209509L4 | Lenti ORF clone of Mcl1 (mGFP-tagged ORF) - Rat myeloid cell leukemia sequence 1 (Mcl1), (10 ug) |
USD 600.00 |
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