Tspan12 (NM_001015026) Rat Tagged Lenti ORF Clone
CAT#: RR200766L4
- LentiORF®
Lenti ORF clone of Tspan12 (mGFP-tagged ORF) - Rat tetraspanin 12 (Tspan12), (10 ug)
"NM_001015026" in other vectors (3)
Specifications
Product Data | |
Type | Rat Tagged ORF Clone |
Tag | mGFP |
Symbol | Tspan12 |
Synonyms | Tm4sf12 |
Vector | pLenti-C-mGFP-P2A-Puro |
E. coli Selection | Chloramphenicol (34 ug/mL) |
Mammalian Cell Selection | Puromycin |
Sequence Data |
The ORF insert of this clone is exactly the same as(RR200766).
|
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_001015026 |
ORF Size | 915 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001015026.1, NP_001015026.1 |
RefSeq Size | 2563 bp |
RefSeq ORF | 918 bp |
Locus ID | 362326 |
UniProt ID | Q569A2 |
Cytogenetics | 4q22 |
Gene Summary | Regulator of cell surface receptor signal transduction. Plays a central role in retinal vascularization by regulating norrin (NDP) signal transduction. Acts in concert with norrin (NDP) to promote FZD4 multimerization and subsequent activation of FZD4, leading to promote accumulation of beta-catenin (CTNNB1) and stimulate LEF/TCF-mediated transcriptional programs. Suprisingly, it only activate the norrin (NDP)-dependent activation of FZD4, while it does not activate the Wnt-dependent activation of FZD4, suggesting the existence of a Wnt-independent signaling that also promote accumulation the beta-catenin (CTNNB1). Acts as a regulator of membrane proteinases such as ADAM10 and MMP14/MT1-MMP. Activates ADAM10-dependent cleavage activity of amyloid precursor protein (APP). Activates MMP14/MT1-MMP-dependent cleavage activity (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RN200766 | Tspan12 (untagged ORF) - Rat tetraspanin 12 (Tspan12), (10 ug) |
USD 330.00 |
|
RR200766 | Tspan12 (Myc-DDK-tagged ORF) - Rat tetraspanin 12 (Tspan12), (10 ug) |
USD 330.00 |
|
RR200766L3 | Lenti ORF clone of Tspan12 (Myc-DDK-tagged ORF) - Rat tetraspanin 12 (Tspan12), (10 ug) |
USD 630.00 |
{0} Product Review(s)
Be the first one to submit a review