Glud1 (NM_012570) Rat Untagged Clone

CAT#: RN215683

Glud1 (untagged ORF) - Rat glutamate dehydrogenase 1 (Glud1), (10 ug)

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Glud1
• Synonyms: Ac2-281; Gdh1; Glud; Gludeha; MRG-2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN215683 representing NM_012570
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTACCGCCGTCTGGGCGAAGTGCTGCTACTGTCCCGCGCCGGGCCCGCTGCCCTGGGCTCTGCGGCTG
  CAGACTCAGCCGCACTGCTGGGCTGGGCTCGCGGACAACCCTCTGCTGTCCCGCAACCCGGGCTCACGCC
  GGTCGCCAGGCGCCACTACAGCGAAGCGGCCACCGACCGCGAAGACGACCCCAACTTCTTCAAGATGGTG
  GAGGGCTTCTTCGACCGCGGCGCCAGCATCGTGGAGGACAAGCTGGTGGAAGACCTGAAGACCCGGGAGA
  ACGAGGAGCAGAAGCGGAACCGAGTGCGCGGCATCCTGCGGATCATCAAGCCTTGCAACCATGTGTTGAG
  CCTCTCCTTCCCCATCCGGCGCGACGACGGCTCCTGGGAGGTCATCGAAGGCTACCGGGCCCAGCACAGC
  CAGCACCGCACGCCCTGCAAGGGAGGTATCCGGTACAGCACTGACGTGAGTGTGGATGAGGTGAAGGCGC
  TGGCGTCCCTAATGACCTACAAGTGTGCAGTGGTTGATGTGCCATTTGGAGGTGCTAAAGCAGGCGTTAA
  GATCAATCCCAAGAACTATACAGATAATGAATTAGAAAAGATTACACGAAGATTCACCATGGAGCTGGCA
  AAGAAGGGTTTTATTGGTCCTGGCATTGATGTGCCTGCCCCAGACATGAGCACGGGCGAGCGGGAGATGT
  CCTGGATCGCTGACACCTATGCCAGCACCATAGGGCACTATGATATCAATGCACACGCCTGTGTCACTGG
  TAAACCCATCAGCCAAGGAGGCATCCACGGACGCATCTCCGCTACTGGCCGGGGTGTTTTTCATGGGATT
  GAGAACTTCATCAATGAAGCTTCCTACATGAGCATCTTAGGGATGACCCCGGGGCTTGGCGATAAGACGT
  TTGTTGTTCAGGGATTTGGTAATGTGGGCCTGCACTCTATGAGATATTTACATCGTTTTGGTGCTAAGTG
  TGTTGGTGTTGGAGAATCTGATGGGAGTATATGGAATCCAGATGGTATTGACCCAAAAGAACTGGAAGAT
  TTCAAGTTGCAACATGGATCAATTCTGGGCTTCCCCAAAGCCAAGGTCTATGAAGGAAGCATCTTGGAGG
  CTGACTGCGACATTTTAATTCCTGCAGCCAGCGAGAAGCAGTTGACCAAATCCAATGCACCCAGAGTCAA
  AGCCAAGATCATTGCTGAAGGAGCCAATGGCCCAACCACTCCAGAGGCCGATAAGATTTTCCTAGAAAGA
  AACATCATGGTTATTCCAGATCTCTACCTGAATGCTGGAGGAGTGACAGTATCTTACTTTGAGTGGCTAA
  AGAATCTAAATCACGTCAGCTATGGCCGATTGACCTTCAAATATGAAAGGGACTCGAACTACCACTTGCT
  CATGTCCGTTCAAGAGAGTTTAGAGAGAAAGTTTGGAAAGCACGGCGGGACTATCCCTGTGGTCCCCACA
  GCAGAGTTCCAGGACAGAATATCGGGTGCATCTGAGAAAGACATCGTGCACTCTGGCTTGGCCTACACAA
  TGGAGCGATCTGCCAGGCAAATTATGCGCACAGCCATGAAGTATAACCTGGGATTGGACCTGAGAACAGC
  TGCCTACGTCAATGCCATTGAGAAAGTCTTCAAGGTGTACAATGAGGCTGGCGTGACCTTCACATAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_012570
• Insert Size: 1677 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_012570.1, NP_036702.1
• RefSeq Size: 2998 bp
• RefSeq ORF: 1677 bp
• Locus ID: 24399
• UniProt ID: P10860
• Cytogenetics: 16p15
• Gene Summary: catalyzes the oxidative deamination of 1-glutamate to 2-oxoglutarate and may be a candidate memory-related gene [RGD, Feb 2006]