Ascl1 (NM_022384) Rat Untagged Clone

SKU
RN215503
Ascl1 (untagged ORF) - Rat achaete-scute complex homolog 1 (Drosophila) (Ascl1), (10 ug)
$330.00
4 Weeks*
Specifications
Product Data
Type Rat Untagged Clone
Target Symbol Ascl1
Synonyms Mash1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>RN215503 representing NM_022384
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGAGCTCTGGCAAGATGGAGAGCGGAGCCGGCCAGCAGCCGCAGCCTCCGCAGCCCTTCCTGCCGC
CTGCAGCCTGCTTCTTTGCGACCGCGGCGGCCGCGGCGGCGGCGGCGGCGGCAGCGGCAGCTCAGAGCGC
GCAGCAGCAACAGCAGCAGCAGGCGCCGCAGCAGCAGGCGCCGCAGCTGAGCCCGGTGGCGGACGGCCAG
CCCTCAGGGGGCGGTCACAAGTCAGCGGCCAAGCAGGTCAAGCGCCAGCGCTCGTCCTCTCCCGAACTGA
TGCGCTGCAAACGCCGGCTCAACTTCAGTGGCTTCGGCTACAGCCTTCCACAGCAGCAGCCGGCAGCCGT
GGCGCGCCGCAACGAGCGCGAGCGCAACCGGGTCAAGTTGGTTAACCTGGGCTTTGCCACCCTCCGGGAG
CATGTCCCCAACGGCGCTGCCAACAAGAAGATGAGCAAGGTGGAGACGCTGCGTTCGGCGGTCGAATACA
TCCGCGCGCTGCAGCAGCTGCTGGACGAGCACGATGCGGTGAGCGCTGCCTTCCAGGCGGGCGTCCTGTC
GCCCACCATCTCCCCCAACTACTCCAACGACTTGAACTCTATGGCGGGTTCTCCGGTTTCGTCCTACTCC
TCCGACGAGGGATCCTACGACCCCCTTAGTCCAGAGGAACAAGAGCTGCTGGACTTTACCAACTGGTTCT
GA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI
ACCN NM_022384
Insert Size 702 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_022384.1, NP_071779.1
RefSeq Size 1656 bp
RefSeq ORF 702 bp
Locus ID 64186
UniProt ID P19359
Cytogenetics 7q13
Summary plays a role in neurogenesis of the autonomic nervous system [RGD, Feb 2006]
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Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.