Clu (NM_053021) Rat Untagged Clone

CAT#: RN214684

Clu (untagged ORF) - Rat clusterin (Clu), (10 ug)

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Clu
• Synonyms: APOJ; CLI; DAG; RATTRPM2B; SGP-2; SGP2; SP-40; SP40; TRPM-2; Trpm2; TRPM2B; Trpmb
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN214684 representing NM_053021
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGATTCTCCTGCTGTGTGTGGCACTGCTGCTGACCTGGGACAATGGCATGGTCCTGGGAGAGCAGG
  AGTTCTCTGACAATGAGCTCCAAGAACTGTCCACTCAAGGAAGTAGGTATGTTAATAAGGAGATTCAGAA
  CGCCGTCCAGGGGGTGAAGCACATAAAGACCCTCATAGAAAAAACCAACGCAGAGCGCAAGTCCCTGCTC
  AACAGTTTAGAGGAAGCCAAAAAGAAGAAAGAGGGTGCTCTAGATGACACCAGGGATTCTGAAATGAAGC
  TGAAGGCTTTCCCGGAAGTGTGTAACGAGACCATGATGGCCCTCTGGGAAGAGTGTAAGCCCTGCCTGAA
  GCACACCTGCATGAAGTTCTACGCACGCGTCTGCAGGAGCGGCTCGGGGCTGGTTGGTCGCCAGCTAGAG
  GAGTTTCTGAACCAGAGCTCACCCTTCTACTTCTGGATGAACGGGGACCGCATCGACTCCCTGCTGGAGA
  GTGACCGGCAGCAGAGCCAAGTCCTAGATGCTATGCAGGACAGCTTCACTCGGGCGTCTGGCATCATACA
  TACGCTTTTCCAGGACCGGTTCTTCACCCATGAGCCCCAGGACATCCACCATTTCTCCCCCATGGGCTTC
  CCACACAAGCGGCCTCATTTCTTGTACCCCAAGTCCCGCTTGGTCCGCAGCCTCATGCCTCTCTCCCACT
  ACGGGCCTCTGAGCTTCCACAACATGTTCCAGCCTTTCTTTGATATGATACACCAGGCTCAACAGGCCAT
  GGACGTCCAGCTCCATAGCCCAGCTTTACAGTTCCCGGATGTGGATTTCTTAAAAGAAGGTGAAGATGAC
  CCGACAGTGTGCAAGGAGATCCGCCATAACTCCACAGGATGCCTGAAGATGAAGGGCCAGTGTGAGAAGT
  GCCAAGAGATCTTGTCTGTGGACTGTTCGACCAACAATCCTGCCCAGGCTAACCTGCGCCAGGAGCTAAA
  CGACTCGCTCCAGGTGGCTGAGAGGCTGACCCAGCAGTACAACGAGCTGCTTCATTCCCTCCAGTCCAAG
  ATGCTCAACACCTCATCCCTGCTGGAACAGCTGAACGACCAGTTCACGTGGGTGTCCCAGCTGGCTAACC
  TCACACAGGGCGATGACCAGTACCTTCGGGTCTCCACAGTGACAACCCATTCTTCTGACTCAGAAGTCCC
  CTCTCGTGTCACTGAGGTGGTGGTGAAGCTGTTTGACTCTGACCCCATCACAGTGGTGTTACCAGAAGAA
  GTCTCCAAGGATAACCCTAAGTTTATGGACACAGTGGCAGAGAAAGCGCTACAGGAATACCGCAGGAAAA
  GCCGCATGGAATGA
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-RsrII
• ACCN: NM_053021
• Insert Size: 1344 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_053021.2, NP_444180.2
• RefSeq Size: 1857 bp
• RefSeq ORF: 1344 bp
• Locus ID: 24854
• UniProt ID: P05371
• Cytogenetics: 15p12
• Gene Summary: The protein encoded by this gene is a secreted chaperone that can under some stress conditions also be found in the cell cytosol. It has been suggested to be involved in several basic biological events such as cell death, tumor progression, and neurodegenerative disorders.[provided by RefSeq, May 2011]