Abhd6 (NM_001007680) Rat Untagged Clone

CAT#: RN212804

Abhd6 (untagged ORF) - Rat abhydrolase domain containing 6 (Abhd6), (10 ug)

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Abhd6
• Synonyms: MGC94917
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN212804 representing NM_001007680
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATCTCGATGTGGTTAACATGTTTGTGATTGCGGGTGGGACCCTGGCCATTCCGATCCTGGCCTTCG
  TAGCTTCTTTCCTCCTTTGGCCCTCGGCGCTGATAAGAATCTATTATTGGTACTGGCGGAGGACGCTGGG
  CATGCAAGTCCGCTACGTGCACCACGAGGACTATCAGTTCTGTTACTCCTTCCGGGGCAGGCCAGGACAC
  AAGCCGTCCGTCCTTATGCTTCACGGGTTCTCCGCACACAAGGATATGTGGCTCAGTGTGGTCAAGTTCC
  TTCCCAAGAATCTGCACTTGGTCTGTGTGGACATGCCTGGACATGAAGGGACCACCCGTTCCTCCCTGGA
  TGACCTGTCCATAGTTGGGCAAGTGAAAAGGATACATCAGTTTGTAGAATGCCTTAAGCTGAACAAAAAG
  CCCTTTCACCTTATAGGCACCTCCATGGGTGGCAACGTGGCTGGAGTATACGCTGCTTACTATCCATCTG
  ACGTCTGCAGCCTGTCTCTCGTGTGCCCTGCTGGCCTGCAGTATTCAACTGACAATCGGTTTGTACAACG
  GCTCAAAGAGCTGGAGGACTCAGCCGCCACGCAGAAGATCCCTTTGATCCCATCCACCCCGGAAGAGATG
  AGCGAGATGCTGCAGCTCTGCTCGTACGTCCGCTTCAAGGTGCCCCAGCAGATCCTTCAAGGTCTTGTCG
  ACGTTCGCATCCCTCACAACAGCTTCTACCGGAAATTGTTTTTGGAAATCGTCAGTGAGAAATCCAGATA
  CTCTCTGCATGAGAACATGGACAAGATCAAGGTCCCAACACAGATCATTTGGGGAAAACAAGACCAGGTG
  CTTGATGTGTCGGGGGCAGACATATTAGCCAAGTCCATCACTAACTCCCAGGTAGAGGTTCTGGAAAACT
  GTGGCCATTCAGTGGTGATGGAGAGACCGAGGAAGACAGCCAAGCTCGTCGTCGACTTTTTAGCTTCTGT
  GCACAACCCAGACAACAACAAGAAGCTGAACTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001007680
• Insert Size: 1014 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001007680.1, NP_001007681.1
• RefSeq Size: 2225 bp
• RefSeq ORF: 1014 bp
• Locus ID: 305795
• UniProt ID: Q5XI64
• Cytogenetics: 15p14
• Gene Summary: Lipase that preferentially hydrolysis medium-chain saturated monoacylglycerols including 2-arachidonoylglycerol (By similarity). Through 2-arachidonoylglycerol degradation may regulate endocannabinoid signaling pathways. Also has a lysophosphatidyl lipase activity with a preference for lysophosphatidylglycerol among other lysophospholipids (By similarity). Also able to degrade bis(monoacylglycero)phosphate (BMP) and constitutes the major enzyme for BMP catabolism. BMP, also known as lysobisphosphatidic acid, is enriched in late endosomes and lysosomes and plays a key role in the formation of intraluminal vesicles and in lipid sorting (By similarity).[UniProtKB/Swiss-Prot Function]