Asic2 (NM_001034014) Rat Untagged Clone

SKU RN200428

Asic2 (untagged ORF) - Rat amiloride-sensitive cation channel 1, neuronal (Accn1), transcript variant 2, (10 ug)

Product Data

• Type: Rat Untagged Clone
• Target Symbol: Asic2
• Synonyms: Accn1; BNaC1; BNC1k; MDEG; MDEG1; MDEG2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN200428 representing NM_001034014; Red=Cloning site Blue=ORF Orange=Stop codon; ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGGACCTCAAGGAGAGCCCCAGTGAGGGCAGCCTGCAACCTTCCAGTATCCAGATCTTCGCCAATACCT; CCACTCTCCATGGCATCCGCCACATCTTCGTGTATGGGCCGCTGACCATCCGGCGTGTGCTTTGGGCAGT; GGCCTTCGTCGGATCCCTGGGCCTGCTGCTGGTGGAGAGCTCGGAGAGGGTGTCCTACTATTTCTCTTAT; CAGCATGTTACCAAGGTGGATGAAGTGGTGGCCCAGAGCCTGGTCTTCCCAGCTGTGACCCTCTGCAACC; TCAATGGCTTCCGGTTCTCCAGGCTTACCACCAACGACTTGTACCACGCTGGGGAGTTGCTGGCCCTGCT; GGATGTCAACCTACAGATTCCCGACCCGCATCTGGCAGACCCCACGGTGCTGGAGGCCCTCCGACAGAAG; GCCAACTTCAAACACTACAAACCGAAGCAGTTCAGCATGCTGGAGTTCCTGCACCGGGTAGGCCATGACC; TGAAGGATATGATGCTCTACTGCAAGTTCAAGGGGCAGGAGTGTGGGCATCAAGACTTCACCACAGTGTT; TACAAAATACGGGAAGTGTTACATGTTTAACTCAGGCGAGGATGGCAAGCCGCTGCTCACCACGGTCAAG; GGGGGGACGGGCAACGGGCTGGAGATCATGCTGGACATTCAGCAAGATGAGTACCTGCCCATCTGGGGAG; AGACAGAGGAAACAACGTTTGAAGCAGGAGTGAAGGTTCAGATCCACAGTCAGTCTGAGCCGCCTTTCAT; CCAAGAGCTGGGCTTTGGGGTGGCTCCGGGGTTCCAGACCTTCGTGGCCACACAAGAGCAGAGGCTCACA; TATCTGCCCCCACCATGGGGGGAGTGCCGGTCCTCAGAGATGGGACTCGACTTCTTTCCTGTTTACAGCA; TCACAGCCTGTCGGATTGACTGTGAGACCCGCTACATCGTGGAGAACTGTAACTGCCGCATGGTCCACAT; GCCAGGGGACGCCCCTTTCTGCACCCCTGAGCAGCACAAGGAGTGTGCAGAGCCTGCCCTCGGTCTACTG; GCAGAAAAGGACAGCAATTACTGTCTCTGCAGGACACCCTGCAACCTGACACGCTACAACAAAGAGCTCT; CCATGGTGAAGATCCCCAGCAAGACGTCAGCCAAGTACTTAGAGAAGAAATTTAACAAATCGGAAAAATA; TATCTCAGAGAACATTCTTGTTCTGGACATATTTTTTGAGGCGCTCAATTACGAAACAATTGAACAGAAG; AAGGCGTATGAAGTTGCTGCCTTACTTGGTGACATCGGTGGTCAGATGGGACTGTTCATTGGTGCTAGTC; TCCTCACAATACTAGAGCTCTTTGATTATATTTATGAGCTGATCAAAGAGAAGCTATTAGACCTGCTTGG; CAAAGAAGAAGAGGAAGGGAGCCACGATGAGAACATGAGCACCTGTGACACAATGCCAAACCACTCTGAA; ACCATCAGCCACACTGTGAACGTGCCCCTGCAGACAGCTTTGGGCACCCTGGAGGAGATTGCCTGCTGA; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001034014
• Insert Size: 1539 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_001034014.1, NP_001029186.1
• RefSeq Size: 2565 bp
• RefSeq ORF: 1539 bp
• Locus ID: 25364
• UniProt ID: Q62962
• Cytogenetics: 10q26
• Summary: member of the degenerin/epithelial Na(+) channel superfamily; permeable for Na(+), K(+) and Li(+); mutations may lead to a form of hereditary neurodegeneration; may be important for pain sensation [RGD, Feb 2006]; Transcript Variant: This variant (MDEG1) uses an alternate 5' terminal exon compared to variant MDEG2. The resulting protein (isoform MDGEG1) is shorter and has a distinct N-terminus compared to isoform MDEG2.