Glypican 5 (GPC5) (NM_004466) Human Tagged ORF Clone

CAT#: RG207909

GPC5 (tGFP-tagged) - Human glypican 5 (GPC5)

Product Images

Circular map for RG207909

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Glypican 5
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG207909 representing NM_004466
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACGCACAGACCTGGCCCGTGGGCTTTCGCTGCCTCCTCCTTCTGGCCCTGGTTGGGTCCGCCCGCA
  GCGAGGGCGTGCAGACCTGCGAAGAAGTTCGGAAACTTTTCCAGTGGCGGCTGCTGGGAGCTGTCAGGGG
  GCTGCCGGATTCGCCGCGGGCAGGACCTGATCTTCAGGTTTGCATATCCAAAAAGCCTACATGTTGCACC
  AGGAAGATGGAGGAGAGATATCAGATTGCGGCTCGCCAGGATATGCAGCAGTTTCTTCAAACGTCCAGCT
  CTACATTAAAGTTTCTAATATCTCGAAATGCGGCTGCTTTTCAAGAAACCCTTGAAACTCTCATCAAACA
  AGCAGAAAATTACACCAGTATACTTTTTTGCAGTACCTACAGGAACATGGCCTTGGAGGCTGCTGCTTCG
  GTTCAGGAGTTCTTCACTGATGTGGGGCTGTATTTATTTGGTGCGGATGTTAATCCTGAAGAATTTGTAA
  ACAGATTTTTTGACAGTCTTTTTCCTCTGGTCTACAACCACCTCATTAACCCTGGTGTGACTGACAGTTC
  CCTGGAATACTCAGAATGCATCCGGATGGCTCGCCGGGATGTGAGTCCATTTGGTAATATTCCCCAAAGA
  GTAATGGGACAGATGGGGAGGTCCCTGCTGCCCAGCCGCACTTTTCTGCAGGCACTCAATCTGGGCATTG
  AAGTCATCAACACCACAGACTATCTGCACTTCTCCAAAGAGTGCAGCAGAGCCCTCCTGAAGATGCAATA
  CTGCCCGCACTGCCAAGGCCTGGCGCTCACTAAGCCTTGTATGGGATACTGCCTCAATGTCATGCGAGGC
  TGCCTGGCGCACATGGCGGAGCTTAATCCACACTGGCATGCATATATCCGGTCGTTGGAAGAACTCTCGG
  ATGCAATGCATGGAACATACGACATTGGACACGTGCTGCTGAACTTTCACTTGCTTGTTAATGATGCTGT
  GTTACAGGCTCACCTCAATGGACAAAAATTATTGGAACAGGTAAATAGGATTTGTGGCCGCCCTGTAAGA
  ACACCCACACAAAGCCCCCGTTGTTCTTTTGATCAGAGCAAAGAGAAGCATGGAATGAAGACCACCACAA
  GGAACAGTGAAGAGACGCTTGCCAACAGAAGAAAAGAATTTATCAACAGCCTTCGACTGTACAGGTCATT
  CTATGGAGGTCTAGCTGATCAGCTTTGTGCTAATGAATTAGCTGCTGCAGATGGACTTCCCTGCTGGAAT
  GGAGAAGATATAGTAAAAAGTTATACTCAGCGTGTGGTTGGAAATGGAATCAAAGCCCAGTCTGGAAATC
  CTGAAGTCAAAGTCAAAGGAATTGATCCTGTGATAAATCAGATTATTGATAAACTGAAGCATGTTGTTCA
  GTTGTTACAGGGTAGATCACCCAAACCTGACAAGTGGGAACTTCTTCAGCTGGGCAGTGGTGGAGGCATG
  GTTGAACAAGTCAGTGGGGACTGTGATGATGAAGATGGTTGCGGGGGATCAGGAAGTGGAGAAGTCAAGA
  GGACACTGAAGATCACAGACTGGATGCCAGATGATATGAACTTCAGTGATGTAAAGCAAATCCATCAAAC
  AGACACTGGCAGTACTTTAGACACAACAGGAGCAGGATGTGCAGTGGCGACTGAATCTATGACATTCACT
  CTGATAAGTGTGGTGATGTTACTTCCCGGGATTTGG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG207909 representing NM_004466
  Red=Cloning site Green=Tags(s)
  
  MDAQTWPVGFRCLLLLALVGSARSEGVQTCEEVRKLFQWRLLGAVRGLPDSPRAGPDLQVCISKKPTCCT
  RKMEERYQIAARQDMQQFLQTSSSTLKFLISRNAAAFQETLETLIKQAENYTSILFCSTYRNMALEAAAS
  VQEFFTDVGLYLFGADVNPEEFVNRFFDSLFPLVYNHLINPGVTDSSLEYSECIRMARRDVSPFGNIPQR
  VMGQMGRSLLPSRTFLQALNLGIEVINTTDYLHFSKECSRALLKMQYCPHCQGLALTKPCMGYCLNVMRG
  CLAHMAELNPHWHAYIRSLEELSDAMHGTYDIGHVLLNFHLLVNDAVLQAHLNGQKLLEQVNRICGRPVR
  TPTQSPRCSFDQSKEKHGMKTTTRNSEETLANRRKEFINSLRLYRSFYGGLADQLCANELAAADGLPCWN
  GEDIVKSYTQRVVGNGIKAQSGNPEVKVKGIDPVINQIIDKLKHVVQLLQGRSPKPDKWELLQLGSGGGM
  VEQVSGDCDDEDGCGGSGSGEVKRTLKITDWMPDDMNFSDVKQIHQTDTGSTLDTTGAGCAVATESMTFT
  LISVVMLLPGIW
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_004466
• ORF Size: 1716 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004466.6
• RefSeq Size: 2934 bp
• RefSeq ORF: 1719 bp
• Locus ID: 2262
• UniProt ID: P78333
• Cytogenetics: 13q31.3
• Domains: Glypican
• Gene Summary: Cell surface heparan sulfate proteoglycans are composed of a membrane-associated protein core substituted with a variable number of heparan sulfate chains. Members of the glypican-related integral membrane proteoglycan family (GRIPS) contain a core protein anchored to the cytoplasmic membrane via a glycosyl phosphatidylinositol linkage. These proteins may play a role in the control of cell division and growth regulation. [provided by RefSeq, Jul 2008]