Glypican 5 (GPC5) (NM_004466) Human Untagged Clone

CAT#: SC324198

GPC5 (untagged)-Human glypican 5 (GPC5)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Glypican 5
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_004466.3 CTGCTGCGAGCCGAGCCGGGCGGCGGCGGCAGTGGCGGCAGTGGCGGCAGCGGCAGCAGT
  TGCAGCAGTGGTGGCCAGAGCGGATGCTTGCGGGCTCCCTGCGGCTCCACTAGTTTTCTT
  CGCCCCGCCCAGCCGCCCACTCTTCTCGGCTAGGGAAGAAGACCAGAGGGTGCTCAGCTG
  GAAAACTCTGGTGTCTCAGCTTAGGGCCTCCTCCGGGAAGAGCTAACTGCTCCCAGGTGA
  AGCCGGTGCCCGCGGGCGGTCCGTACACCCCGCAGCCGGCTCGCACCGCTCGAGAGCCTC
  GGCCGCTGTGTCTTCCACGTCTGCAGCTCAGCCAGGGCGCGCAGGGCGAGTGGGGTCCAC
  TGGCGGGTAAAGGGGACCAGGACGGCGAGGATGGACGCACAGACCTGGCCCGTGGGCTTT
  CGCTGCCTCCTCCTTCTGGCCCTGGTTGGGTCCGCCCGCAGCGAGGGCGTGCAGACCTGC
  GAAGAAGTTCGGAAACTTTTCCAGTGGCGGCTGCTGGGAGCTGTCAGGGGGCTGCCGGAT
  TCGCCGCGGGCAGGACCTGATCTTCAGGTTTGCATATCCAAAAAGCCTACATGTTGCACC
  AGGAAGATGGAGGAGAGATATCAGATTGCGGCTCGCCAGGATATGCAGCAGTTTCTTCAA
  ACGTCCAGCTCTACATTAAAGTTTCTAATATCTCGAAATGCGGCTGCTTTTCAAGAAACC
  CTTGAAACTCTCATCAAACAAGCAGAAAATTACACCAGTATACTTTTTTGCAGTACCTAC
  AGGAACATGGCCTTGGAGGCTGCTGCTTCGGTTCAGGAGTTCTTCACTGATGTGGGGCTG
  TATTTATTTGGTGCGGATGTTAATCCTGAAGAATTTGTAAACAGATTTTTTGACAGTCTT
  TTTCCTCTGGTCTACAACCACCTCATTAACCCTGGTGTGACTGACAGTTCCCTGGAATAC
  TCAGAATGCATCCGGATGGCTCGCCGGGATGTGAGTCCATTTGGTAATATTCCCCAAAGA
  GTAATGGGACAGATGGGGAGGTCCCTGCTGCCCAGCCGCACTTTTCTGCAGGCACTCAAT
  CTGGGCATTGAAGTCATCAACACCACAGACTATCTGCACTTCTCCAAAGAGTGCAGCAGA
  GCCCTCCTGAAGATGCAATACTGCCCGCACTGCCAAGGCCTGGCGCTCACTAAGCCTTGT
  ATGGGATACTGCCTCAATGTCATGCGAGGCTGCCTGGCGCACATGGCGGAGCTTAATCCA
  CACTGGCATGCATATATCCGGTCGTTGGAAGAACTCTCGGATGCAATGCATGGAACATAC
  GACATTGGACACGTGCTGCTGAACTTTCACTTGCTTGTTAATGATGCTGTGTTACAGGCT
  CACCTCAATGGACAAAAATTATTGGAACAGGTAAATAGGATTTGTGGCCGCCCTGTAAGA
  ACACCCACACAAAGCCCCCGTTGTTCTTTTGATCAGAGCAAAGAGAAGCATGGAATGAAG
  ACCACCACAAGGAACAGTGAAGAGACGCTTGCCAACAGAAGAAAAGAATTTATCAACAGC
  CTTCGACTGTACAGGTCATTCTATGGAGGTCTAGCTGATCAGCTTTGTGCTAATGAATTA
  GCTGCTGCAGATGGACTTCCCTGCTGGAATGGAGAAGATATAGTAAAAAGTTATACTCAG
  CGTGTGGTTGGAAATGGAATCAAAGCCCAGTCTGGAAATCCTGAAGTCAAAGTCAAAGGA
  ATTGATCCTGTGATAAATCAGATTATTGATAAACTGAAGCATGTTGTTCAGTTGTTACAG
  GGTAGATCACCCAAACCTGACAAGTGGGAACTTCTTCAGCTGGGCAGTGGTGGAGGCATG
  GTTGAACAAGTCAGTGGGGACTGTGATGATGAAGATGGTTGCGGGGGATCAGGAAGTGGA
  GAAGTCAAGAGGACACTGAAGATCACAGACTGGATGCCAGATGATATGAACTTCAGTGAT
  GTAAAGCAAATCCATCAAACAGACACTGGCAGTACTTTAGACACAACAGGAGCAGGATGT
  GCAGTGGCGACTGAATCTATGACATTCACTCTGATAAGTGTGGTGATGTTACTTCCCGGG
  ATTTGGTAACTGAACTCTTCTGTCCTGACATACCTTACTGAAGTCTCGATTTCTTCTCTC
  TCTGCATATGCCTGGAATAAGAGATCCTTTTTCAATGTAACAATTATATTTATGAAAAGA
  TATGTTACACTAACTTCTCAGAAGCCAAGCTGAAATATTCATAAAGTCCCTAAAACTCAA
  CGTTTAAATGACACACTTTAAAAATATGTCTTTTTTCAATCTAACTGAAAACCTTCTTAA
  CTTCTAATATATTAAATCTGAAGATGTGAAGGGCACAGAAGTGACTTTGAATAAGAAGAA
  TTTAGTGTATCTGTAATTTTATTATCAATTCCAAGCCCCTTCCTTTCTAAATTAAAAATG
  TTTTCATTTGAAAGTGTATTTGCCAGACAATGAAAACAGTATGCAGTATTTCTTAAAGTA
  TTGAAATTAGAATATCATGAAATAAATCAAAACATACAATGGCAAGTAGTATGCATGCAT
  ATTCAAGAGACTCTTCCATTTTTGCAAGCTGTAGAAGGAAATGTCTGAATGTCTATAAGT
  TATGGGGTAGATTCTTGAGAAGCATTTCATATAATTTCACTGAAGAACCTTGATAATTTT
  GACCCACTGTAACTTAGCCACTGATGAACCTTAAAGCTGAGTATTTTATTAACACCTGAT
  TTGTATTCCATTATATTCAAAATGCATCTTTGGTATTGTGCCTCTGCTCCCATCTCTCTC
  TTTGCCTCATAGATTTAGCTATGTTGGGAAGCACATGCTTGCTCTAGGAATATCTCCAAT
  AAAGCTGTTAACTATTTGGTGGAAAAAAAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: ECoRI-NOT
• ACCN: NM_004466
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004466.3, NP_004457.1
• RefSeq Size: 2934 bp
• RefSeq ORF: 1719 bp
• Locus ID: 2262
• UniProt ID: P78333
• Cytogenetics: 13q31.3
• Domains: Glypican
• Gene Summary: Cell surface heparan sulfate proteoglycans are composed of a membrane-associated protein core substituted with a variable number of heparan sulfate chains. Members of the glypican-related integral membrane proteoglycan family (GRIPS) contain a core protein anchored to the cytoplasmic membrane via a glycosyl phosphatidylinositol linkage. These proteins may play a role in the control of cell division and growth regulation. [provided by RefSeq, Jul 2008]