Glypican 5 (GPC5) (NM_004466) Human Untagged Clone

CAT#: SC117378

GPC5 (untagged)-Human glypican 5 (GPC5)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Glypican 5
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC117378 sequence for NM_004466 edited (data generated by NextGen Sequencing)
  ATGGACGCACAGACCTGGCCCGTGGGCTTTCGCTGCCTCCTCCTTCTGGCCCTGGTTGGG
  TCCGCCCGCAGCGAGGGCGTGCAGACCTGCGAAGAAGTTCGGAAACTTTTCCAGTGGCGG
  CTGCTGGGAGCTGTCAGGGGGCTGCCGGATTCGCCGCGGGCAGGACCTGATCTTCAGGTT
  TGCATATCCAAAAAGCCTACATGTTGCACCAGGAAGATGGAGGAGAGATATCAGATTGCG
  GCTCGCCAGGATATGCAGCAGTTTCTTCAAACGTCCAGCTCTACATTAAAGTTTCTAATA
  TCTCGAAATGCGGCTGCTTTTCAAGAAACCCTTGAAACTCTCATCAAACAAGCAGAAAAT
  TACACCAGTATACTTTTTTGCAGTACCTACAGGAACATGGCCTTGGAGGCTGCTGCTTCG
  GTTCAGGAGTTCTTCACTGATGTGGGGCTGTATTTATTTGGTGCGGATGTTAATCCTGAA
  GAATTTGTAAACAGATTTTTTGACAGTCTTTTTCCTCTGGTCTACAACCACCTCATTAAC
  CCTGGTGTGACTGACAGTTCCCTGGAATACTCAGAATGCATCCGGATGGCTCGCCGGGAT
  GTGAGTCCATTTGGTAATATTCCCCAAAGAGTAATGGGACAGATGGGGAGGTCCCTGCTG
  CCCAGCCGCACTTTTCTGCAGGCACTCAATCTGGGCATTGAAGTCATCAACACCACAGAC
  TATCTGCACTTCTCCAAAGAGTGCAGCAGAGCCCTCCTGAAGATGCAATACTGCCCGCAC
  TGCCAAGGCCTGGCGCTCACTAAGCCTTGTATGGGATACTGCCTCAATGTCATGCGAGGC
  TGCCTGGCGCACATGGCGGAGCTTAATCCACACTGGCATGCATATATCCGGTCGTTGGAA
  GAACTCTCGGATGCAATGCATGGAACATACGACATTGGACACGTGCTGCTGAACTTTCAC
  TTGCTTGTTAATGATGCTGTGTTACAGGCTCACCTCAATGGACAAAAATTATTGGAACAG
  GTAAATAGGATTTGTGGCCGCCCTGTAAGAACACCCACACAAAGCCCCCGTTGTTCTTTT
  GATCAGAGCAAAGAGAAGCATGGAATGAAGACCACCACAAGGAACAGTGAAGAGACGCTT
  GCCAACAGAAGAAAAGAATTTATCAACAGCCTTCGACTGTACAGGTCATTCTATGGAGGT
  CTAGCTGATCAGCTTTGTGCTAATGAATTAGCTGCTGCAGATGGACTTCCCTGCTGGAAT
  GGAGAAGATATAGTAAAAAGTTATACTCAGCGTGTGGTTGGAAATGGAATCAAAGCCCAG
  TCTGGAAATCCTGAAGTCAAAGTCAAAGGAATTGATCCTGTGATAAATCAGATTATTGAT
  AAACTGAAGCATGTTGTTCAGTTGTTACAGGGTAGATCACCCAAACCTGACAAGTGGGAA
  CTTCTTCAGCTGGGCAGTGGTGGAGGCATGGTTGAACAAGTCAGTGGGGACTGTGATGAT
  GAAGATGGTTGCGGGGGATCAGGAAGTGGAGAAGTCAAGAGGACACTGAAGATCACAGAC
  TGGATGCCAGATGATATGAACTTCAGTGATGTAAAGCAAATCCATCAAACAGACACTGGC
  AGTACTTTAGACACAACAGGAGCAGGATGTGCAGTGGCGACTGAATCTATGACATTCACT
  CTGATAAGTGTGGTGATGTTACTTCCCGGGATTTGGTAA
  
  Clone variation with respect to NM_004466.4
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004466 unedited
  GTCCCCATTTGTATACGACTCATATAGGGCGGCCGCGAATTCGCACGAGGCGTACACCCC
  GCAGCCGGCTCGCACCGCTCGAGAGCCTCGGCCGCTGTGTCTTCCACGTCTGCAGCTCAG
  CCAGGGCGCGCAGGGCGAGTGGGGTCCACTGGCGGGTAAAGGGGACCAGGACGGCGAGGA
  TGGACGCACAGACCTGGCCCGTGGGCTTTCGCTGCCTCCTCCTTCTGGCCCTGGTTGGGT
  CCGCCCGCAGCGAGGGCGTGCAGACCTGCGAAGAAGTTCGGAAACTTTTCCAGTGGCGGC
  TGCTGGGAGCTGTCAGGGGGCTGCCGGATTCGCCGCGGGCAGGACCTGATCTTCAGGTTT
  GCATATCCAAAAAGCCTACATGTTGCACCAGGAAGATGGAGGAGAGATATCAGATTGCGG
  CTCGCCAGGATATGCAGCAGTTTCTTCAAACGTCCAGCTCTACATTAAAGTTTCTAATAT
  CTCGAAATGCGGCTGCTTTTCAAGAAACCCTTGAAACTCTCATCAAACAAGCCGAAAATT
  ACACCAGTATACTTTTTTGCAGTACCTACAGGAACATGGCCTTGGAGGCTGCTGCTTCGG
  TTCAGGAGTTCTTCACTGATGTGGGGCTGTATTTATTTGGTGCGGATGTTAATCCTGAAG
  ATTTGNTAACAGATTTTTTGACAGTCTTTTTCCTCTGGTCTACAACCACCTCATTAACCC
  TGGTGTGACTGACAGTTCCCTGGAATACTCANAATGCATNCGGATGGCTCGCCGGGATGT
  GAGTCCATTTTGTAATATTCCCCAAAGAGTATGGGACAGAGGGGNAGTCCCTGCTGCCCA
  GCCCACTTTTCTGCAGGACTCAATCTGGGCATTGAATCATCACACCACAGACTTCTGCAC
  TCTTCAAGATGCAGCAGAGCCCTCTGAGATGCATACATGCG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_004466 unedited
  TTCTTGGATATATCTATGNNACCGCGGCCGCATNCTANGATCGAGTTTTTTTTTTTTTTT
  TTTTTTTTTTTTTTTTTTTTTTTTTTTCCACCAAATAGTTAACAGCTTTATTGGAAATAT
  TCCTAGAGCAAGCATGTGCTTCCCAACATAGCTAAATCTATGAGGCAAAAAGAGAGATGG
  GAGCAAAGGCCCAATACCAAAGATGCATTTTGAATATAAGGGAATACAAATCAGGGGTTA
  ATAAAATACTCAGCTTTAAGGTTCATCAGGGGCTAAGTTACAGGGGGTCAAAATTATCAA
  GGTTCTTCAGGGAAATTATATGAAATGCTTCTCAAAAATCTACCCCATAACTTATAGACA
  TTCAAACATTTCCTTCTACAGCTTGCAAAAATGGAAAAGTCTCTTGAATATGCATGCATA
  CTACTTGCCATTGGATGTTTTGATTTATTTCATGATATTCTAATTTCAATACTTTAAAAA
  ATACTGCATACTGTTTTCATTGGCTGGCAAATACACTTTCAAATGAAAACATTTTTAATT
  TAAAAAGGAAGGGGCTTGGAATTGATAATAAAATTACAGATCCCCTAAATTCTTCTTATT
  CAAAGTCACTTCTGTGCCCTTCACATCTTCAGATTTAATATATTAAAAGTTAAGAAGGTT
  TTCAGTTAGATTGAAAAAAGACATATTTTTAAAGTGTGTCATTTAAACGTTGAGTTTTAG
  GGACTTTATGAATATTTCAGCTTGGCTTCTGAAAAGTTAGTGTAACATATCTTTTCATAA
  AATAATTGTTACATTGAAAAAGATCTCTTTATCCAGGCCTATGCAAAAAGGGAAGAAATC
  GAGACTTTGTTAGGTATTTTCAGGACAGAAAAATTCATA
  
• Restriction Sites: ECoRI-NOT
• ACCN: NM_004466
• Insert Size: 2500 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004466.3, NP_004457.1
• RefSeq Size: 2934 bp
• RefSeq ORF: 1719 bp
• Locus ID: 2262
• UniProt ID: P78333
• Cytogenetics: 13q31.3
• Domains: Glypican
• Gene Summary: Cell surface heparan sulfate proteoglycans are composed of a membrane-associated protein core substituted with a variable number of heparan sulfate chains. Members of the glypican-related integral membrane proteoglycan family (GRIPS) contain a core protein anchored to the cytoplasmic membrane via a glycosyl phosphatidylinositol linkage. These proteins may play a role in the control of cell division and growth regulation. [provided by RefSeq, Jul 2008]