GPR183 (NM_004951) Human Tagged ORF Clone
CAT#: RG204736
- TrueORF®
GPR183 (tGFP-tagged) - Human G protein-coupled receptor 183 (GPR183)
ORF Plasmid: DDK
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
AAV Particle: DDK
"NM_004951" in other vectors (5)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Symbol | GPR183 |
Synonyms | EBI2; hEBI2 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG204736 representing NM_004951
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATATACAAATGGCAAACAATTTTACTCCGCCCTCTGCAACTCCTCAGGGAAATGACTGTGACCTCT ATGCACATCACAGCACGGCCAGGATAGTAATGCCTCTGCATTACAGCCTCGTCTTCATCATTGGGCTCGT GGGAAACTTACTAGCCTTGGTCGTCATTGTTCAAAACAGGAAAAAAATCAACTCTACCACCCTCTATTCA ACAAATTTGGTGATTTCTGATATACTTTTTACCACCGCTTTGCCTACACGAATAGCCTACTATGCAATGG GCTTTGACTGGAGAATCGGAGATGCCTTGTGTAGGATAACTGCGCTAGTGTTTTACATCAACACATATGC AGGTGTGAACTTTATGACCTGCCTGAGTATTGACCGCTTCATTGCTGTGGTGCACCCTCTACGCTACAAC AAGATAAAAAGGATTGAACATGCAAAAGGCGTGTGCATATTTGTCTGGATTCTAGTATTTGCTCAGACAC TCCCACTCCTCATCAACCCTATGTCAAAGCAGGAGGCTGAAAGGATTACATGCATGGAGTATCCAAACTT TGAAGAAACTAAATCTCTTCCCTGGATTCTGCTTGGGGCATGTTTCATAGGATATGTACTTCCACTTATA ATCATTCTCATCTGCTATTCTCAGATCTGCTGCAAACTCTTCAGAACTGCCAAACAAAACCCACTCACTG AGAAATCTGGTGTAAACAAAAAGGCTCTCAACACAATTATTCTTATTATTGTTGTGTTTGTTCTCTGTTT CACACCTTACCATGTTGCAATTATTCAACATATGATTAAGAAGCTTCGTTTCTCTAATTTCCTGGAATGT AGCCAAAGACATTCGTTCCAGATTTCTCTGCACTTTACAGTATGCCTGATGAACTTCAATTGCTGCATGG ACCCTTTTATCTACTTCTTTGCATGTAAAGGGTATAAGAGAAAGGTTATGAGGATGCTGAAACGGCAAGT CAGTGTATCGATTTCTAGTGCTGTGAAGTCAGCCCCTGAAGAAAATTCACGTGAAATGACAGAAACGCAG ATGATGATACATTCCAAGTCTTCAAATGGAAAG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG204736 representing NM_004951
Red=Cloning site Green=Tags(s) MDIQMANNFTPPSATPQGNDCDLYAHHSTARIVMPLHYSLVFIIGLVGNLLALVVIVQNRKKINSTTLYS TNLVISDILFTTALPTRIAYYAMGFDWRIGDALCRITALVFYINTYAGVNFMTCLSIDRFIAVVHPLRYN KIKRIEHAKGVCIFVWILVFAQTLPLLINPMSKQEAERITCMEYPNFEETKSLPWILLGACFIGYVLPLI IILICYSQICCKLFRTAKQNPLTEKSGVNKKALNTIILIIVVFVLCFTPYHVAIIQHMIKKLRFSNFLEC SQRHSFQISLHFTVCLMNFNCCMDPFIYFFACKGYKRKVMRMLKRQVSVSISSAVKSAPEENSREMTETQ MMIHSKSSNGK TRTRPLE - GFP Tag - V |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_004951 |
ORF Size | 1083 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_004951.5 |
RefSeq Size | 1746 bp |
RefSeq ORF | 1086 bp |
Locus ID | 1880 |
UniProt ID | P32249 |
Cytogenetics | 13q32.3 |
Domains | 7tm_1 |
Protein Families | Druggable Genome, GPCR, Transmembrane |
Gene Summary | This gene was identified by the up-regulation of its expression upon Epstein-Barr virus infection of primary B lymphocytes. This gene is predicted to encode a G protein-coupled receptor that is most closely related to the thrombin receptor. Expression of this gene was detected in B-lymphocyte cell lines and lymphoid tissues but not in T-lymphocyte cell lines or peripheral blood T lymphocytes. The function of this gene is unknown. [provided by RefSeq, Jul 2008] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC204736 | GPR183 (Myc-DDK-tagged)-Human G protein-coupled receptor 183 (GPR183) |
USD 457.00 |
|
RC204736L3 | Lenti ORF clone of Human G protein-coupled receptor 183 (GPR183), Myc-DDK-tagged |
USD 757.00 |
|
RC204736L4 | Lenti ORF clone of Human G protein-coupled receptor 183 (GPR183), mGFP tagged |
USD 757.00 |
|
SC117047 | GPR183 (untagged)-Human G protein-coupled receptor 183 (GPR183) |
USD 457.00 |
|
SC324529 | GPR183 (untagged)-Human G protein-coupled receptor 183 (GPR183) |
USD 457.00 |
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