GPR183 (NM_004951) Human Tagged ORF Clone

CAT#: RC204736

GPR183 (Myc-DDK-tagged)-Human G protein-coupled receptor 183 (GPR183)

Product Images

Circular map for RC204736

• 

  Western blot validation of overexpression lysate (Cat# LY401538) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC204736 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: GPR183
• Synonyms: EBI2; hEBI2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC204736 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATATACAAATGGCAAACAATTTTACTCCGCCCTCTGCAACTCCTCAGGGAAATGACTGTGACCTCT
  ATGCACATCACAGCACGGCCAGGATAGTAATGCCTCTGCATTACAGCCTCGTCTTCATCATTGGGCTCGT
  GGGAAACTTACTAGCCTTGGTCGTCATTGTTCAAAACAGGAAAAAAATCAACTCTACCACCCTCTATTCA
  ACAAATTTGGTGATTTCTGATATACTTTTTACCACCGCTTTGCCTACACGAATAGCCTACTATGCAATGG
  GCTTTGACTGGAGAATCGGAGATGCCTTGTGTAGGATAACTGCGCTAGTGTTTTACATCAACACATATGC
  AGGTGTGAACTTTATGACCTGCCTGAGTATTGACCGCTTCATTGCTGTGGTGCACCCTCTACGCTACAAC
  AAGATAAAAAGGATTGAACATGCAAAAGGCGTGTGCATATTTGTCTGGATTCTAGTATTTGCTCAGACAC
  TCCCACTCCTCATCAACCCTATGTCAAAGCAGGAGGCTGAAAGGATTACATGCATGGAGTATCCAAACTT
  TGAAGAAACTAAATCTCTTCCCTGGATTCTGCTTGGGGCATGTTTCATAGGATATGTACTTCCACTTATA
  ATCATTCTCATCTGCTATTCTCAGATCTGCTGCAAACTCTTCAGAACTGCCAAACAAAACCCACTCACTG
  AGAAATCTGGTGTAAACAAAAAGGCTCTCAACACAATTATTCTTATTATTGTTGTGTTTGTTCTCTGTTT
  CACACCTTACCATGTTGCAATTATTCAACATATGATTAAGAAGCTTCGTTTCTCTAATTTCCTGGAATGT
  AGCCAAAGACATTCGTTCCAGATTTCTCTGCACTTTACAGTATGCCTGATGAACTTCAATTGCTGCATGG
  ACCCTTTTATCTACTTCTTTGCATGTAAAGGGTATAAGAGAAAGGTTATGAGGATGCTGAAACGGCAAGT
  CAGTGTATCGATTTCTAGTGCTGTGAAGTCAGCCCCTGAAGAAAATTCACGTGAAATGACAGAAACGCAG
  ATGATGATACATTCCAAGTCTTCAAATGGAAAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC204736 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MDIQMANNFTPPSATPQGNDCDLYAHHSTARIVMPLHYSLVFIIGLVGNLLALVVIVQNRKKINSTTLYS
  TNLVISDILFTTALPTRIAYYAMGFDWRIGDALCRITALVFYINTYAGVNFMTCLSIDRFIAVVHPLRYN
  KIKRIEHAKGVCIFVWILVFAQTLPLLINPMSKQEAERITCMEYPNFEETKSLPWILLGACFIGYVLPLI
  IILICYSQICCKLFRTAKQNPLTEKSGVNKKALNTIILIIVVFVLCFTPYHVAIIQHMIKKLRFSNFLEC
  SQRHSFQISLHFTVCLMNFNCCMDPFIYFFACKGYKRKVMRMLKRQVSVSISSAVKSAPEENSREMTETQ
  MMIHSKSSNGK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_004951
• ORF Size: 1083 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004951.5
• RefSeq Size: 1801 bp
• RefSeq ORF: 1086 bp
• Locus ID: 1880
• UniProt ID: P32249
• Cytogenetics: 13q32.3
• Domains: 7tm_1
• Protein Families: Druggable Genome, GPCR, Transmembrane
• MW: 41.2 kDa
• Gene Summary: This gene was identified by the up-regulation of its expression upon Epstein-Barr virus infection of primary B lymphocytes. This gene is predicted to encode a G protein-coupled receptor that is most closely related to the thrombin receptor. Expression of this gene was detected in B-lymphocyte cell lines and lymphoid tissues but not in T-lymphocyte cell lines or peripheral blood T lymphocytes. The function of this gene is unknown. [provided by RefSeq, Jul 2008]