GPR183 (NM_004951) Human Untagged Clone

CAT#: SC324529

GPR183 (untagged)-Human G protein-coupled receptor 183 (GPR183)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: GPR183
• Synonyms: EBI2; hEBI2
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_004951.3 ACTTCAGTTTGGACAACTACTCACAGCTACTACACAGAGACCCGAACGAGTCACTGATAT
  ACACCTGGACCACCACCAATGGATATACAAATGGCAAACAATTTTACTCCGCCCTCTGCA
  ACTCCTCAGGGAAATGACTGTGACCTCTATGCACATCACAGCACGGCCAGGATAGTAATG
  CCTCTGCATTACAGCCTCGTCTTCATCATTGGGCTCGTGGGAAACTTACTAGCCTTGGTC
  GTCATTGTTCAAAACAGGAAAAAAATCAACTCTACCACCCTCTATTCAACAAATTTGGTG
  ATTTCTGATATACTTTTTACCACCGCTTTGCCTACACGAATAGCCTACTATGCAATGGGC
  TTTGACTGGAGAATCGGAGATGCCTTGTGTAGGATAACTGCGCTAGTGTTTTACATCAAC
  ACATATGCAGGTGTGAACTTTATGACCTGCCTGAGTATTGACCGCTTCATTGCTGTGGTG
  CACCCTCTACGCTACAACAAGATAAAAAGGATTGAACATGCAAAAGGCGTGTGCATATTT
  GTCTGGATTCTAGTATTTGCTCAGACACTCCCACTCCTCATCAACCCTATGTCAAAGCAG
  GAGGCTGAAAGGATTACATGCATGGAGTATCCAAACTTTGAAGAAACTAAATCTCTTCCC
  TGGATTCTGCTTGGGGCATGTTTCATAGGATATGTACTTCCACTTATAATCATTCTCATC
  TGCTATTCTCAGATCTGCTGCAAACTCTTCAGAACTGCCAAACAAAACCCACTCACTGAG
  AAATCTGGTGTAAACAAAAAGGCTCTCAACACAATTATTCTTATTATTGTTGTGTTTGTT
  CTCTGTTTCACACCTTACCATGTTGCAATTATTCAACATATGATTAAGAAGCTTCGTTTC
  TCTAATTTCCTGGAATGTAGCCAAAGACATTCGTTCCAGATTTCTCTGCACTTTACAGTA
  TGCCTGATGAACTTCAATTGCTGCATGGACCCTTTTATCTACTTCTTTGCATGTAAAGGG
  TATAAGAGAAAGGTTATGAGGATGCTGAAACGGCAAGTCAGTGTATCGATTTCTAGTGCT
  GTGAAGTCAGCCCCTGAAGAAAATTCACGTGAAATGACAGAAACGCAGATGATGATACAT
  TCCAAGTCTTCAAATGGAAAGTGAAATGGATTGTATTTTGGTTTATAGTGACGTAAACTG
  TATGACAAACTTTGCAGGACTTCCCTTATAAAGCAAAATAATTGTTCAGCTTCCAATTAG
  TATTCTTTTATATTTCTTTCATTGGGCACTTTCCCATCTCCAACTCGGAAGTAAGCCCAA
  GAGAACAACATAAAGCAAACAACATAAAGCACAATAAAAATGCAAATAAATATTTCATTT
  TTATTTGTAAACGAATACACCAAAAGGAGGCGCTCTTAATAACTCCCAATGTAAAAAGTT
  TTGTTTTAATAAAAAATTTAATTATTATTTCTTGCCAACAAATGGCTAGAAAGGACTGAA
  TAGATTATATATTGCCAGATGTTAATACTGTAACATACTTTTTAAATAACATATTTCTTA
  AATCCAAATTTCTCTCAATGTTAGATTTAATTCCCTCAATAACACCAATGTTTTGTTTTG
  TTTCGTTCTGGGTCATAAAACTTTGTTAAGGAACTCTTTTGGAATAAAGAGCAGGATGCT
  ACAAAAAAAAAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_004951
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004951.3, NP_004942.1
• RefSeq Size: 1746 bp
• RefSeq ORF: 1086 bp
• Locus ID: 1880
• UniProt ID: P32249
• Cytogenetics: 13q32.3
• Domains: 7tm_1
• Protein Families: Druggable Genome, GPCR, Transmembrane
• Gene Summary: This gene was identified by the up-regulation of its expression upon Epstein-Barr virus infection of primary B lymphocytes. This gene is predicted to encode a G protein-coupled receptor that is most closely related to the thrombin receptor. Expression of this gene was detected in B-lymphocyte cell lines and lymphoid tissues but not in T-lymphocyte cell lines or peripheral blood T lymphocytes. The function of this gene is unknown. [provided by RefSeq, Jul 2008]