START domain containing 7 (STARD7) (NM_020151) Human Tagged ORF Clone

CAT#: RG202539

STARD7 (tGFP-tagged) - Human StAR-related lipid transfer (START) domain containing 7 (STARD7)

Product Images

Circular map for RG202539

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: START domain containing 7
• Synonyms: FAME2; GTT1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG202539 representing NM_020151
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCGTTAGCCGGCGTCTTCGTTTGGGACGAGGAGAGGATCCAGGAGGAGGAGTTGCAGAGATCTA
  TTAATGAGATGAAGCGGTTGGAAGAAATGTCAAATATGTTTCAGAGCTCTGGAGTCCAGCACCACCCTCC
  AGAACCAAAAGCCCAAACAGAAGGGAATGAAGATTCAGAGGGCAAAGAGCAACGTTGGGAAATGGTGATG
  GATAAGAAACACTTTAAGCTGTGGCGGCGCCCAATTACAGGCACCCACCTTTACCAGTACCGAGTTTTTG
  GAACCTACACAGATGTGACACCTCGGCAGTTCTTCAATGTTCAGCTGGACACAGAGTATAGAAAAAAATG
  GGATGCCCTGGTAATCAAGCTGGAGGTGATTGAGAGGGATGTGGTTAGTGGTTCCGAGGTTCTTCACTGG
  GTAACCCATTTTCCTTATCCAATGTACTCACGGGATTATGTTTATGTTCGGCGGTATAGTGTGGATCAGG
  AAAACAACATGATGGTGTTGGTGTCGCGTGCTGTGGAGCATCCGAGTGTGCCAGAGTCTCCAGAATTCGT
  CAGGGTCAGATCATATGAATCCCAAATGGTTATCCGTCCCCACAAGTCATTTGATGAGAATGGCTTTGAC
  TACTTACTAACATACAGTGACAATCCCCAAACGGTGTTTCCTCGCTACTGTGTTAGTTGGATGGTTTCCA
  GTGGCATGCCAGATTTCCTGGAGAAGCTGCACATGGCCACTCTGAAAGCCAAGAATATGGAGATTAAAGT
  AAAGGACTACATCTCAGCTAAGCCTCTGGAAATGAGTAGTGAAGCCAAGGCCACCAGCCAGTCCTCTGAG
  CGAAAGAACGAGGGCAGCTGTGGCCCTGCTCGGATTGAGTATGCT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG202539 representing NM_020151
  Red=Cloning site Green=Tags(s)
  
  MAALAGVFVWDEERIQEEELQRSINEMKRLEEMSNMFQSSGVQHHPPEPKAQTEGNEDSEGKEQRWEMVM
  DKKHFKLWRRPITGTHLYQYRVFGTYTDVTPRQFFNVQLDTEYRKKWDALVIKLEVIERDVVSGSEVLHW
  VTHFPYPMYSRDYVYVRRYSVDQENNMMVLVSRAVEHPSVPESPEFVRVRSYESQMVIRPHKSFDENGFD
  YLLTYSDNPQTVFPRYCVSWMVSSGMPDFLEKLHMATLKAKNMEIKVKDYISAKPLEMSSEAKATSQSSE
  RKNEGSCGPARIEYA
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_020151
• ORF Size: 885 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_020151.2, NP_064536.1
• RefSeq Size: 3394 bp
• RefSeq ORF: 1113 bp
• Locus ID: 56910
• UniProt ID: Q9NQZ5
• Cytogenetics: 2q11.2
• Domains: START
• Gene Summary: May play a protective role in mucosal tissues by preventing exaggerated allergic responses.[UniProtKB/Swiss-Prot Function]