nkx6.1 (NKX6-1) (NM_006168) Human Tagged ORF Clone

CAT#: RC224762

NKX6 (Myc-DDK-tagged)-Human NK6 homeobox 1 (NKX6-1)

Product Images

Circular map for RC224762

• 

  Western blot validation of overexpression lysate (Cat# LY401859) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC224762 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: nkx6.1
• Synonyms: NKX6.1; NKX6A
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC224762 representing NM_006168
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTAGCGGTGGGGGCAATGGAGGGCACCCGGCAGAGCGCATTCCTGCTCAGCAGCCCTCCCCTGGCCG
  CCCTGCACAGCATGGCCGAGATGAAGACCCCGCTGTACCCTGCCGCGTATCCCCCGCTGCCTGCCGGCCC
  CCCCTCCTCCTCGTCCTCGTCGTCGTCCTCCTCGTCGCCCTCCCCGCCTCTGGGCACCCACAACCCAGGC
  GGCCTGAAGCCCCCGGCCACGGGGGGGCTCTCATCCCTCGGCAGCCCCCCGCAGCAGCTCTCGGCCGCCA
  CCCCACACGGCATCAACGATATCCTGAGCCGGCCCTCCATGCCCGTGGCCTCGGGGGCCGCCCTGCCCTC
  CGCCTCGCCCTCCGGTTCCTCCTCCTCCTCTTCCTCGTCCGCCTCTGCCTCCTCCGCCTCTGCCGCCGCC
  GCGGCTGCTGCCGCGGCCGCAGCCGCCGCCTCATCCCCGGCGGGGCTGCTGGCCGGACTGCCACGCTTTA
  GCAGCCTGAGCCCGCCGCCGCCGCCGCCCGGGCTCTACTTCAGCCCCAGCGCCGCGGCCGTGGCCGCCGT
  GGGCCGGTACCCCAAGCCGCTGGCTGAGCTGCCTGGCCGGACGCCCATCTTCTGGCCCGGAGTGATGCAG
  AGCCCGCCCTGGAGGGACGCACGCCTGGCCTGTACCCCTCATCAAGGATCCATTTTGTTGGACAAAGACG
  GGAAGAGAAAACACACGAGACCCACTTTTTCCGGACAGCAGATCTTCGCCCTGGAGAAGACTTTCGAACA
  AACAAAATACTTGGCGGGGCCCGAGAGGGCTCGTTTGGCCTATTCGTTGGGGATGACAGAGAGTCAGGTC
  AAGGTCTGGTTCCAGAACCGCCGGACCAAGTGGAGGAAGAAGCACGCTGCCGAGATGGCCACGGCCAAGA
  AGAAGCAGGACTCGGAGACAGAGCGCCTCAAGGGGGCCTCGGAGAACGAGGAAGAGGACGACGACTACAA
  TAAGCCTCTGGATCCCAACTCGGACGACGAGAAAATCACGCAGCTGTTGAAGAAGCACAAGTCCAGCAGC
  GGCGGCGGCGGCGGCCTCCTACTGCACGCGTCCGAGCCGGAGAGCTCATCC
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC224762 representing NM_006168
  Red=Cloning site Green=Tags(s)
  
  MLAVGAMEGTRQSAFLLSSPPLAALHSMAEMKTPLYPAAYPPLPAGPPSSSSSSSSSSSPSPPLGTHNPG
  GLKPPATGGLSSLGSPPQQLSAATPHGINDILSRPSMPVASGAALPSASPSGSSSSSSSSASASSASAAA
  AAAAAAAAAASSPAGLLAGLPRFSSLSPPPPPPGLYFSPSAAAVAAVGRYPKPLAELPGRTPIFWPGVMQ
  SPPWRDARLACTPHQGSILLDKDGKRKHTRPTFSGQQIFALEKTFEQTKYLAGPERARLAYSLGMTESQV
  KVWFQNRRTKWRKKHAAEMATAKKKQDSETERLKGASENEEEDDDYNKPLDPNSDDEKITQLLKKHKSSS
  GGGGGLLLHASEPESSS
  
  SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_006168
• ORF Size: 1101 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006168.2, NP_006159.2
• RefSeq Size: 1116 bp
• RefSeq ORF: 1104 bp
• Locus ID: 4825
• UniProt ID: P78426
• Cytogenetics: 4q21.23
• Protein Families: Transcription Factors
• Protein Pathways: Maturity onset diabetes of the young
• MW: 37.7 kDa
• Gene Summary: In the pancreas, NKX6.1 is required for the development of beta cells and is a potent bifunctional transcription regulator that binds to AT-rich sequences within the promoter region of target genes Iype et al. (2004) [PubMed 15056733].[supplied by OMIM, Mar 2008]