ACCN5 (ASIC5) (NM_017419) Human Tagged ORF Clone

CAT#: RC223871

ASIC5 (Myc-DDK-tagged)-Human amiloride-sensitive cation channel 5, intestinal (ACCN5)

Product Images

Circular map for RC223871

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  Western blot validation of overexpression lysate (Cat# LY413777) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC223871 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified ASIC5 protein (Cat# TP323871). The protein was produced from HEK293T cells transfected with ASIC5 cDNA clone (Cat# RC223871) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ACCN5
• Synonyms: ACCN5; HINAC; INAC
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC223871 representing NM_017419
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCAGACAGAAAAATCAAAAGTATATGCTGAGAATGGACTCTTAGAAAAGATAAAGCTTTGCCCTT
  CAAAGAAACCACTGCCATCTCCCACTGAGCGAAAGAAGTTTGACTATGACTTTGCCATCTCCACTTCCTT
  TCATGGGATACACAATATTGTTCAGAACCGGAGCAAAATTCGCAGGGTGCTCTGGTTGGTGGTGGTTCTG
  GGCTCAGTCTCACTTGTGACATGGCAGATCTACATTCGCTTGCTCAACTACTTCACATGGCCAACCACAG
  CGTCCATTGAGGTTCAATATGTGGAAAAGATGGAGTTCCCAACTGTGACATTTTGTAATTTGAACAGGTT
  TCAAACAGATGCTGTAGCCAAATTTGGTGTTATTTTTTTCTTATGGCACATTGTATCCAAAGTCCTCCAT
  CTTCAAGAAATTACTGCCAATTCCACTGGCTCTAGAGAGGCTACTGATTTTGCTGCAAGTCACCAAAACT
  TCAGCATTGTGGAATTTATCAGGAACAAAGGTTTTTATCTCAACAATAGCACTTTGTTGGACTGTGAGTT
  TTTTGGAAAGCCATGTAGCCCAAAGGATTTTGCACATGTCTTCACTGAATATGGAAATTGTTTTACTTTT
  AATCATGGTGAAACTCTCCAAGCAAAGAGAAAAGTGAGTGTCTCTGGAAGAGGTTTGAGCTTACTCTTCA
  ATGTGAATCAGGAGGCATTCACTGATAACCCAGCCCTTGGTTTCGTTGATGCTGGGATCATCTTTGTTAT
  CCATTCACCAAAGAAGGTGCCACAGTTTGATGGGTTAGGCTTGTTGTCACCTGTGGGAATGCACGCAAGG
  GTAACCATCCGCCAAGTGAAGACAGTTCATCAAGAATACCCTTGGGGAGAATGCAATCCTAACATCAAGC
  TGCAGAATTTTAGCAGCTACAGCACTTCTGGTTGCTTGAAGGAATGCAAAGCCCAGCACATAAAAAAGCA
  ATGTGGATGTGTGCCTTTTCTTCTTCCTGGATATGGGATAGAATGTGACCTACAAAAGTACTTCAGCTGT
  GTTTCTCCTGTACTTGACCACATTGAATTTAAGGATTTATGTACAGTAGGAACACATAACTCTAGCTGCC
  CCGTTTCTTGTGAAGAAATAGAATACCCGGCCACTATTTCTTATTCCTCTTTTCCAAGTCAAAAAGCTTT
  GAAATATCTTTCCAAGAAGTTGAATCAAAGCCGGAAATACATCAGGGAGAATCTTGTAAAAATTGAAATT
  AACTATAGTGACCTAAACTATAAGATAACCCAGCAGCAAAAGGCGGTGAGTGTGTCTGAGTTACTTGCAG
  ATCTTGGTGGTCAGCTGGGTCTATTTTGTGGGGCCAGTCTGATCACGATCATAGAAATTATTGAATATCT
  ATTCACCAATTTCTACTGGATATGCATTTTCTTTTTGCTGAAGATATCTGAAATGACCCAGTGGACTCCT
  CCACCTCAGAATCATCTGGGAAATAAAAATAGGATAGAGGAGTGT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC223871 representing NM_017419
  Red=Cloning site Green=Tags(s)
  
  MEQTEKSKVYAENGLLEKIKLCPSKKPLPSPTERKKFDYDFAISTSFHGIHNIVQNRSKIRRVLWLVVVL
  GSVSLVTWQIYIRLLNYFTWPTTASIEVQYVEKMEFPTVTFCNLNRFQTDAVAKFGVIFFLWHIVSKVLH
  LQEITANSTGSREATDFAASHQNFSIVEFIRNKGFYLNNSTLLDCEFFGKPCSPKDFAHVFTEYGNCFTF
  NHGETLQAKRKVSVSGRGLSLLFNVNQEAFTDNPALGFVDAGIIFVIHSPKKVPQFDGLGLLSPVGMHAR
  VTIRQVKTVHQEYPWGECNPNIKLQNFSSYSTSGCLKECKAQHIKKQCGCVPFLLPGYGIECDLQKYFSC
  VSPVLDHIEFKDLCTVGTHNSSCPVSCEEIEYPATISYSSFPSQKALKYLSKKLNQSRKYIRENLVKIEI
  NYSDLNYKITQQQKAVSVSELLADLGGQLGLFCGASLITIIEIIEYLFTNFYWICIFFLLKISEMTQWTP
  PPQNHLGNKNRIEEC
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_017419
• ORF Size: 1515 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_017419.3
• RefSeq Size: 1692 bp
• RefSeq ORF: 1518 bp
• Locus ID: 51802
• UniProt ID: Q9NY37
• Cytogenetics: 4q32.1
• Protein Families: Druggable Genome, Ion Channels: Other, Transmembrane
• MW: 57.3 kDa
• Gene Summary: This gene belongs to the amiloride-sensitive Na+ channel and degenerin (NaC/DEG) family, members of which have been identified in many animal species ranging from the nematode to human. The amiloride-sensitive Na(+) channel encoded by this gene is primarily expressed in the small intestine, however, its exact function is not known. [provided by RefSeq, Jul 2008]