LRIG1 (NM_015541) Human Tagged ORF Clone

CAT#: RC223584

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LRIG1 (Myc-DDK-tagged)-Human leucine-rich repeats and immunoglobulin-like domains 1 (LRIG1)



Reconstitution Protocol

USD 951.00

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Size
    • 10 ug

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Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol LRIG1
Synonyms LIG-1; LIG1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC223584 representing NM_015541
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGCGGCCGGTCCGGGGAGGGCTCGGGGCCCCGCGCCGCTCGCCTTGCCTTCTCCTTCTCTGGCTGC
TTTTGCTTCGGCTGGAGCCGGTGACCGCCGCGGCCGGCCCGCGGGCGCCCTGCGCGGCCGCCTGCACTTG
CGCTGGGGACTCGCTGGACTGCGGTGGGCGCGGGCTGGCTGCGTTGCCCGGGGACCTGCCCTCCTGGACG
CGGAGCCTAAACCTGAGTTACAACAAACTCTCTGAGATTGACCCTGCTGGTTTTGAGGACTTGCCGAACC
TACAGGAAGTGTACCTCAATAATAATGAGTTGACAGCGGTACCATCCCTGGGCGCTGCTTCATCACATGT
CGTCTCTCTCTTTCTGCAGCACAACAAGATTCGCAGCGTGGAGGGGAGCCAGCTGAAGGCCTACCTTTCC
TTAGAAGTGTTAGATCTGAGTTTGAACAACATCACGGAAGTGCGGAACACCTGCTTTCCACACGGACCGC
CTATAAAGGAGCTCAACCTGGCAGGCAATCGGATTGGCACCCTGGAGTTGGGAGCATTTGATGGTCTGTC
ACGGTCGCTGCTAACTCTTCGCCTGAGCAAAAACAGGATCACCCAGCTTCCTGTAAGAGCATTCAAGCTA
CCCAGGCTGACACAACTGGACCTCAATCGGAACAGGATTCGGCTGATAGAGGGCCTCACCTTCCAGGGGC
TCAACAGCTTGGAGGTGCTGAAGCTTCAGCGAAACAACATCAGCAAACTGACAGATGGGGCCTTCTGGGG
ACTGTCCAAGATGCATGTGCTGCACCTGGAGTACAACAGCCTGGTAGAAGTGAACAGCGGCTCGCTCTAC
GGCCTCACGGCCCTGCATCAGCTCCACCTCAGCAACAATTCCATCGCTCGCATTCACCGCAAGGGCTGGA
GCTTCTGCCAGAAGCTGCATGAGTTGGTCCTGTCCTTCAACAACCTGACACGGCTGGACGAGGAGAGCCT
GGCCGAGCTGAGCAGCCTGAGTGTCCTGCGTCTCAGCCACAATTCCATCAGCCACATTGCGGAGGGTGCC
TTCAAGGGACTCAGGAGCCTGCGAGTCTTGGATCTGGACCATAACGAGATTTCGGGCACAATAGAGGACA
CGAGCGGCGCCTTCTCAGGGCTCGACAGCCTCAGCAAGCTGACTCTGTTTGGAAACAAGATCAAGTCTGT
GGCTAAGAGAGCATTCTCGGGGCTGGAAGGCCTGGAGCACCTGAACCTTGGAGGGAATGCGATCAGATCT
GTCCAGTTTGATGCCTTTGTGAAGATGAAGAATCTTAAAGAGCTCCATATCAGCAGCGACAGCTTCCTGT
GTGACTGCCAGCTGAAGTGGCTGCCCCCGTGGCTAATTGGCAGGATGCTGCAGGCCTTTGTGACAGCCAC
CTGTGCCCACCCAGAATCACTGAAGGGTCAGAGCATTTTCTCTGTGCCACCAGAGAGTTTCGTGTGCGAT
GACTTCCTGAAGCCACAGATCATCACCCAGCCAGAAACCACCATGGCTATGGTGGGCAAGGACATCCGGT
TTACATGCTCAGCAGCCAGCAGCAGCAGCTCCCCCATGACCTTTGCCTGGAAGAAAGACAATGAAGTCCT
GACCAATGCAGACATGGAGAACTTTGTCCACGTCCACGCGCAGGACGGGGAAGTGATGGAGTACACCACC
ATCCTGCACCTCCGTCAGGTCACTTTCGGGCACGAGGGCCGCTACCAATGTGTCATCACCAACCACTTTG
GCTCCACCTATTCACATAAGGCCAGGCTCACCGTGAATGTGTTGCCATCATTCACCAAAACGCCCCACGA
CATAACCATCCGGACCACCACCATGGCCCGCCTCGAATGTGCTGCCACAGGTCACCCAAACCCTCAGATT
GCCTGGCAGAAGGATGGAGGCACGGATTTCCCCGCTGCCCGTGAGCGACGCATGCATGTCATGCCGGATG
ACGACGTGTTTTTCATCACTGATGTGAAAATAGATGACGCAGGGGTTTACAGCTGTACTGCTCAGAACTC
AGCCGGTTCTATTTCAGCTAATGCCACCCTGACTGTCCTAGAGACCCCATCCTTGGTGGTCCCCTTGGAA
GACCGTGTGGTATCTGTGGGAGAAACAGTGGCCCTCCAATGCAAAGCCACGGGGAACCCTCCGCCCCGCA
TCACCTGGTTCAAGGGGGACCGCCCGCTGAGCCTCACTGAGCGGCACCACTTGACCCCTGACAACCAGCT
CCTGGTGGTTCAGAACGTGGTGGCAGAGGATGCGGGCCGATATACCTGTGAGATGTCCAACACCCTGGGC
ACGGAGCGAGCTCACAGCCAGCTGAGCGTCCTGCCCGCAGCAGGCTGCAGGAAGGATGGGACCACGGTAG
GCATCTTCACCATTGCTGTCGTGAGCAGCATCGTCCTGACGTCACTGGTCTGGGTGTGCATCATCTACCA
GACCAGGAAGAAGAGTGAAGAGTACAGTGTCACCAACACAGATGAAACCGTCGTGCCACCAGATGTTCCA
AGCTACCTCTCTTCTCAGGGGACCCTTTCTGACCGACAAGAAACCGTGGTCAGGACCGAGGGTGGCCCTC
AGGCCAATGGGCACATTGAGAGCAATGGTGTGTGTCCAAGAGATGCAAGCCACTTTCCAGAGCCCGACAC
TCACAGCGTTGCCTGCAGGCAGCCAAAGCTCTGTGCTGGGTCTGCGTATCACAAAGAGCCGTGGAAAGCG
ATGGAGAAAGCTGAAGGGACACCTGGGCCACATAAGATGGAACACGGTGGCCGGGTCGTATGCAGTGACT
GCAACACCGAAGTGGACTGTTACTCCAGGGGACAAGCCTTCCACCCCCAGCCTGTGTCCAGAGACAGCGC
ACAGCCAAGTGCGCCAAATGGCCCGGAGCCGGGTGGGAGTGACCAAGAGCATTCTCCACATCACCAGTGC
AGCAGGACTGCCGCTGGGTCCTGCCCCGAGTGCCAAGGGTCGCTCTACCCCAGTAACCACGATAGAATGC
TGACGGCTGTGAAGAAAAAGCCAATGGCATCTCTAGATGGGAAAGGGGATTCTTCCTGGACTTTAGCAAG
GTTGTATCACCCGGACTCCACAGAGCTACAGCCTGCATCTTCATTAACTTCAGGCAGTCCAGAGCGCGCG
GAAGCCCAGTACTTGCTTGTTTCCAATGGCCACCTCCCCAAAGCATGTGACGCCAGTCCCGAGTCCACGC
CACTGACAGGACAGCTCCCCGGGAAACAGAGGGTGCCACTGCTGTTGGCACCAAAAAGC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC223584 representing NM_015541
Red=Cloning site Green=Tags(s)

MARPVRGGLGAPRRSPCLLLLWLLLLRLEPVTAAAGPRAPCAAACTCAGDSLDCGGRGLAALPGDLPSWT
RSLNLSYNKLSEIDPAGFEDLPNLQEVYLNNNELTAVPSLGAASSHVVSLFLQHNKIRSVEGSQLKAYLS
LEVLDLSLNNITEVRNTCFPHGPPIKELNLAGNRIGTLELGAFDGLSRSLLTLRLSKNRITQLPVRAFKL
PRLTQLDLNRNRIRLIEGLTFQGLNSLEVLKLQRNNISKLTDGAFWGLSKMHVLHLEYNSLVEVNSGSLY
GLTALHQLHLSNNSIARIHRKGWSFCQKLHELVLSFNNLTRLDEESLAELSSLSVLRLSHNSISHIAEGA
FKGLRSLRVLDLDHNEISGTIEDTSGAFSGLDSLSKLTLFGNKIKSVAKRAFSGLEGLEHLNLGGNAIRS
VQFDAFVKMKNLKELHISSDSFLCDCQLKWLPPWLIGRMLQAFVTATCAHPESLKGQSIFSVPPESFVCD
DFLKPQIITQPETTMAMVGKDIRFTCSAASSSSSPMTFAWKKDNEVLTNADMENFVHVHAQDGEVMEYTT
ILHLRQVTFGHEGRYQCVITNHFGSTYSHKARLTVNVLPSFTKTPHDITIRTTTMARLECAATGHPNPQI
AWQKDGGTDFPAARERRMHVMPDDDVFFITDVKIDDAGVYSCTAQNSAGSISANATLTVLETPSLVVPLE
DRVVSVGETVALQCKATGNPPPRITWFKGDRPLSLTERHHLTPDNQLLVVQNVVAEDAGRYTCEMSNTLG
TERAHSQLSVLPAAGCRKDGTTVGIFTIAVVSSIVLTSLVWVCIIYQTRKKSEEYSVTNTDETVVPPDVP
SYLSSQGTLSDRQETVVRTEGGPQANGHIESNGVCPRDASHFPEPDTHSVACRQPKLCAGSAYHKEPWKA
MEKAEGTPGPHKMEHGGRVVCSDCNTEVDCYSRGQAFHPQPVSRDSAQPSAPNGPEPGGSDQEHSPHHQC
SRTAAGSCPECQGSLYPSNHDRMLTAVKKKPMASLDGKGDSSWTLARLYHPDSTELQPASSLTSGSPERA
EAQYLLVSNGHLPKACDASPESTPLTGQLPGKQRVPLLLAPKS

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_015541
ORF Size 3279 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_015541.3
RefSeq Size 4812 bp
RefSeq ORF 3282 bp
Locus ID 26018
UniProt ID Q96JA1
Cytogenetics 3p14.1
Domains LRRNT, LRRCT, LRR, ig, LRR_TYP, IGc2, IG, LRR_SD22, LRR_PS
Protein Families Transmembrane
MW 118.9 kDa
Gene Summary Acts as a feedback negative regulator of signaling by receptor tyrosine kinases, through a mechanism that involves enhancement of receptor ubiquitination and accelerated intracellular degradation.[UniProtKB/Swiss-Prot Function]
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