LRIG1 (NM_015541) Human Tagged ORF Clone

CAT#: RC223584

LRIG1 (Myc-DDK-tagged)-Human leucine-rich repeats and immunoglobulin-like domains 1 (LRIG1)

Product Images

Circular map for RC223584

• 

  Western blot validation of overexpression lysate (Cat# LY414487) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC223584 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified LRIG1 protein (Cat# TP323584). The protein was produced from HEK293T cells transfected with LRIG1 cDNA clone (Cat# RC223584) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: LRIG1
• Synonyms: LIG-1; LIG1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC223584 representing NM_015541
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGCGGCCGGTCCGGGGAGGGCTCGGGGCCCCGCGCCGCTCGCCTTGCCTTCTCCTTCTCTGGCTGC
  TTTTGCTTCGGCTGGAGCCGGTGACCGCCGCGGCCGGCCCGCGGGCGCCCTGCGCGGCCGCCTGCACTTG
  CGCTGGGGACTCGCTGGACTGCGGTGGGCGCGGGCTGGCTGCGTTGCCCGGGGACCTGCCCTCCTGGACG
  CGGAGCCTAAACCTGAGTTACAACAAACTCTCTGAGATTGACCCTGCTGGTTTTGAGGACTTGCCGAACC
  TACAGGAAGTGTACCTCAATAATAATGAGTTGACAGCGGTACCATCCCTGGGCGCTGCTTCATCACATGT
  CGTCTCTCTCTTTCTGCAGCACAACAAGATTCGCAGCGTGGAGGGGAGCCAGCTGAAGGCCTACCTTTCC
  TTAGAAGTGTTAGATCTGAGTTTGAACAACATCACGGAAGTGCGGAACACCTGCTTTCCACACGGACCGC
  CTATAAAGGAGCTCAACCTGGCAGGCAATCGGATTGGCACCCTGGAGTTGGGAGCATTTGATGGTCTGTC
  ACGGTCGCTGCTAACTCTTCGCCTGAGCAAAAACAGGATCACCCAGCTTCCTGTAAGAGCATTCAAGCTA
  CCCAGGCTGACACAACTGGACCTCAATCGGAACAGGATTCGGCTGATAGAGGGCCTCACCTTCCAGGGGC
  TCAACAGCTTGGAGGTGCTGAAGCTTCAGCGAAACAACATCAGCAAACTGACAGATGGGGCCTTCTGGGG
  ACTGTCCAAGATGCATGTGCTGCACCTGGAGTACAACAGCCTGGTAGAAGTGAACAGCGGCTCGCTCTAC
  GGCCTCACGGCCCTGCATCAGCTCCACCTCAGCAACAATTCCATCGCTCGCATTCACCGCAAGGGCTGGA
  GCTTCTGCCAGAAGCTGCATGAGTTGGTCCTGTCCTTCAACAACCTGACACGGCTGGACGAGGAGAGCCT
  GGCCGAGCTGAGCAGCCTGAGTGTCCTGCGTCTCAGCCACAATTCCATCAGCCACATTGCGGAGGGTGCC
  TTCAAGGGACTCAGGAGCCTGCGAGTCTTGGATCTGGACCATAACGAGATTTCGGGCACAATAGAGGACA
  CGAGCGGCGCCTTCTCAGGGCTCGACAGCCTCAGCAAGCTGACTCTGTTTGGAAACAAGATCAAGTCTGT
  GGCTAAGAGAGCATTCTCGGGGCTGGAAGGCCTGGAGCACCTGAACCTTGGAGGGAATGCGATCAGATCT
  GTCCAGTTTGATGCCTTTGTGAAGATGAAGAATCTTAAAGAGCTCCATATCAGCAGCGACAGCTTCCTGT
  GTGACTGCCAGCTGAAGTGGCTGCCCCCGTGGCTAATTGGCAGGATGCTGCAGGCCTTTGTGACAGCCAC
  CTGTGCCCACCCAGAATCACTGAAGGGTCAGAGCATTTTCTCTGTGCCACCAGAGAGTTTCGTGTGCGAT
  GACTTCCTGAAGCCACAGATCATCACCCAGCCAGAAACCACCATGGCTATGGTGGGCAAGGACATCCGGT
  TTACATGCTCAGCAGCCAGCAGCAGCAGCTCCCCCATGACCTTTGCCTGGAAGAAAGACAATGAAGTCCT
  GACCAATGCAGACATGGAGAACTTTGTCCACGTCCACGCGCAGGACGGGGAAGTGATGGAGTACACCACC
  ATCCTGCACCTCCGTCAGGTCACTTTCGGGCACGAGGGCCGCTACCAATGTGTCATCACCAACCACTTTG
  GCTCCACCTATTCACATAAGGCCAGGCTCACCGTGAATGTGTTGCCATCATTCACCAAAACGCCCCACGA
  CATAACCATCCGGACCACCACCATGGCCCGCCTCGAATGTGCTGCCACAGGTCACCCAAACCCTCAGATT
  GCCTGGCAGAAGGATGGAGGCACGGATTTCCCCGCTGCCCGTGAGCGACGCATGCATGTCATGCCGGATG
  ACGACGTGTTTTTCATCACTGATGTGAAAATAGATGACGCAGGGGTTTACAGCTGTACTGCTCAGAACTC
  AGCCGGTTCTATTTCAGCTAATGCCACCCTGACTGTCCTAGAGACCCCATCCTTGGTGGTCCCCTTGGAA
  GACCGTGTGGTATCTGTGGGAGAAACAGTGGCCCTCCAATGCAAAGCCACGGGGAACCCTCCGCCCCGCA
  TCACCTGGTTCAAGGGGGACCGCCCGCTGAGCCTCACTGAGCGGCACCACTTGACCCCTGACAACCAGCT
  CCTGGTGGTTCAGAACGTGGTGGCAGAGGATGCGGGCCGATATACCTGTGAGATGTCCAACACCCTGGGC
  ACGGAGCGAGCTCACAGCCAGCTGAGCGTCCTGCCCGCAGCAGGCTGCAGGAAGGATGGGACCACGGTAG
  GCATCTTCACCATTGCTGTCGTGAGCAGCATCGTCCTGACGTCACTGGTCTGGGTGTGCATCATCTACCA
  GACCAGGAAGAAGAGTGAAGAGTACAGTGTCACCAACACAGATGAAACCGTCGTGCCACCAGATGTTCCA
  AGCTACCTCTCTTCTCAGGGGACCCTTTCTGACCGACAAGAAACCGTGGTCAGGACCGAGGGTGGCCCTC
  AGGCCAATGGGCACATTGAGAGCAATGGTGTGTGTCCAAGAGATGCAAGCCACTTTCCAGAGCCCGACAC
  TCACAGCGTTGCCTGCAGGCAGCCAAAGCTCTGTGCTGGGTCTGCGTATCACAAAGAGCCGTGGAAAGCG
  ATGGAGAAAGCTGAAGGGACACCTGGGCCACATAAGATGGAACACGGTGGCCGGGTCGTATGCAGTGACT
  GCAACACCGAAGTGGACTGTTACTCCAGGGGACAAGCCTTCCACCCCCAGCCTGTGTCCAGAGACAGCGC
  ACAGCCAAGTGCGCCAAATGGCCCGGAGCCGGGTGGGAGTGACCAAGAGCATTCTCCACATCACCAGTGC
  AGCAGGACTGCCGCTGGGTCCTGCCCCGAGTGCCAAGGGTCGCTCTACCCCAGTAACCACGATAGAATGC
  TGACGGCTGTGAAGAAAAAGCCAATGGCATCTCTAGATGGGAAAGGGGATTCTTCCTGGACTTTAGCAAG
  GTTGTATCACCCGGACTCCACAGAGCTACAGCCTGCATCTTCATTAACTTCAGGCAGTCCAGAGCGCGCG
  GAAGCCCAGTACTTGCTTGTTTCCAATGGCCACCTCCCCAAAGCATGTGACGCCAGTCCCGAGTCCACGC
  CACTGACAGGACAGCTCCCCGGGAAACAGAGGGTGCCACTGCTGTTGGCACCAAAAAGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC223584 representing NM_015541
  Red=Cloning site Green=Tags(s)
  
  MARPVRGGLGAPRRSPCLLLLWLLLLRLEPVTAAAGPRAPCAAACTCAGDSLDCGGRGLAALPGDLPSWT
  RSLNLSYNKLSEIDPAGFEDLPNLQEVYLNNNELTAVPSLGAASSHVVSLFLQHNKIRSVEGSQLKAYLS
  LEVLDLSLNNITEVRNTCFPHGPPIKELNLAGNRIGTLELGAFDGLSRSLLTLRLSKNRITQLPVRAFKL
  PRLTQLDLNRNRIRLIEGLTFQGLNSLEVLKLQRNNISKLTDGAFWGLSKMHVLHLEYNSLVEVNSGSLY
  GLTALHQLHLSNNSIARIHRKGWSFCQKLHELVLSFNNLTRLDEESLAELSSLSVLRLSHNSISHIAEGA
  FKGLRSLRVLDLDHNEISGTIEDTSGAFSGLDSLSKLTLFGNKIKSVAKRAFSGLEGLEHLNLGGNAIRS
  VQFDAFVKMKNLKELHISSDSFLCDCQLKWLPPWLIGRMLQAFVTATCAHPESLKGQSIFSVPPESFVCD
  DFLKPQIITQPETTMAMVGKDIRFTCSAASSSSSPMTFAWKKDNEVLTNADMENFVHVHAQDGEVMEYTT
  ILHLRQVTFGHEGRYQCVITNHFGSTYSHKARLTVNVLPSFTKTPHDITIRTTTMARLECAATGHPNPQI
  AWQKDGGTDFPAARERRMHVMPDDDVFFITDVKIDDAGVYSCTAQNSAGSISANATLTVLETPSLVVPLE
  DRVVSVGETVALQCKATGNPPPRITWFKGDRPLSLTERHHLTPDNQLLVVQNVVAEDAGRYTCEMSNTLG
  TERAHSQLSVLPAAGCRKDGTTVGIFTIAVVSSIVLTSLVWVCIIYQTRKKSEEYSVTNTDETVVPPDVP
  SYLSSQGTLSDRQETVVRTEGGPQANGHIESNGVCPRDASHFPEPDTHSVACRQPKLCAGSAYHKEPWKA
  MEKAEGTPGPHKMEHGGRVVCSDCNTEVDCYSRGQAFHPQPVSRDSAQPSAPNGPEPGGSDQEHSPHHQC
  SRTAAGSCPECQGSLYPSNHDRMLTAVKKKPMASLDGKGDSSWTLARLYHPDSTELQPASSLTSGSPERA
  EAQYLLVSNGHLPKACDASPESTPLTGQLPGKQRVPLLLAPKS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_015541
• ORF Size: 3279 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_015541.3
• RefSeq Size: 4812 bp
• RefSeq ORF: 3282 bp
• Locus ID: 26018
• UniProt ID: Q96JA1
• Cytogenetics: 3p14.1
• Domains: LRRNT, LRRCT, LRR, ig, LRR_TYP, IGc2, IG, LRR_SD22, LRR_PS
• Protein Families: Transmembrane
• MW: 118.9 kDa
• Gene Summary: Acts as a feedback negative regulator of signaling by receptor tyrosine kinases, through a mechanism that involves enhancement of receptor ubiquitination and accelerated intracellular degradation.[UniProtKB/Swiss-Prot Function]