CCDC144A (NM_014695) Human Tagged ORF Clone

CAT#: RC221755

CCDC144A (Myc-DDK-tagged)-Human coiled-coil domain containing 144A (CCDC144A)

Product Images

Circular map for RC221755

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CCDC144A
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC221755 representing NM_014695
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCTCCTGGGGTGGAGAAAAGCGGGGAGGGGCTGAGGGGTCTCCGAAGCCGGCAGTCTACGCCACGA
  GGAAGACCCCTAGCGTCGGGAGCCAGGGGGACCAGTGGTACTTGGGCTACCCGGGGGACCAGTGGTCCTC
  GGGCTTCCCCTACAGCTGGTGGAAAAACAGCGTCGGCAGCGAGAGCAAGCACGGTGAGGGCGCCTTAGAC
  CAGCCCCAGCACGACGTCCGCCTGGAAGATCTTGGCGAGCTCCACAGAGCTGCCCGGTCGGGCGACGTCC
  CTGGGGTGGAGCACATCTTAGCTCCTGGAGACACTGGCGTGGACAAGAGGGATAGGAAGAAGAGCATTCA
  GCAACTAGTTCCTGAATATAAGGAAAAACAGACACCTGAAAGTCTTCCTCAAAATAACAATCCAGATTGG
  CATCCTACTAATTTGACCCTTAGTGATGAGACTTGTCAGAGATCCAAGAATCTGAAAGTTGATGATAAAT
  GTCCATCTGTATCACCATCAATGCCTGAAAATCAGTCAGCAACCAAAGAACTGGGACAGATGAACTTAAC
  AGAACGAGAAAAGATGGACACTGGAGTTGTACTTCTCTCAGGGAATGATACTCTCCATGACCTGTGCCAA
  TCACAGCTACCAGAAAACAAAGAGAGCAAAGAAGCAGAACAAGACTCGGAGCTGACATCAGAGGAAGAGC
  AAGAAAGACTTAAAGGATGCGAAAATAAGCAGCCACAGAAAACGTCTCAAGAACCAGAAATGGCTAAGGA
  TTGCGATAGAGAGGATATACCTATATATCCAGTACTTCCTCATGTGCAAAAATCTGAGGAAATGTGGATT
  GAACAAGGCAAATTAGAGTGGAAAAACCAATTAAAACTCGTCATAAATGAGTTAAAGCAGAGGTTTGGTG
  AAATTTATGAAAAATACAAAATTCCGGCTTGTCCTGAGGAAGAGCCACTACTTGATAACTCTACAAGAGG
  AACAGATGTAAAGGATATTCCCTTTAATTTGACAAATAACATACCTGGTTGTGAGGAAGAAGATGCATCT
  GAAATATCTGTCTCAGTGGTATTCGAGACATTTCCTGAACAAAAAGAACCCAGTCTCAAAAATATCATCC
  ATCCATACTATCATCCATACTCTGGGTCCCAGGAACATGTTTGCCAGTCATCTTCTAAGTTTCATTTACA
  TGAAAATAAATTAGACTGCGACAATGATAACAAACCAGGCATTGGACATATTTTTAGTACAGATAAGAAC
  TTTCATAATGATGCAAGCACTAAGAAAGCAAGGAACCCAGAAGTGGTTATGGTTGAAATGAAAGAAGACC
  AAGAGTTTGATTTGCAAATGACAAAAAATATGAACCAAAATAGTGACAGTGGCAGTACAAACAACTATAA
  AAGCCTGAAACCTAAATTAGAAAATCTGAGTTCTTTACCACCAGATTCTGACAGAACATCAGAAGTATAT
  CTACATGAAGAATTACAGCAAGACATGCAAAAGTTTAAGAATGAGGTCAACACATTAGAAGAAGAGTTCC
  TGGCTTTGAAGAAAGAAGATGTTCAACTTCATAAAGATGTTGAAGAAGAAATGGAGAAGCACAGAAGTAA
  TAGCACAGAATTATCAGGAACCCTAACTGATGGTACTACTGTTGGCAATGATGATGATGGACTAAATCAG
  CAGATTCCTAGGAAGGAAAATGGAGAGCATGACAGGCCTGCAGATAAAACATCTAATGAAAAGAACGAGG
  TCAAAAACCAAATATATCCTGAGGCTGACTTTGCTGACTCAATGGAGCCATCTGAAATAGCCTCAGAGGA
  TTGTGAATTGTCTCACTCTGTTTATGAGAATTTTATGTTGCTGATTGAACAACTTAGAATGGAGTATAAA
  GATTCTGCTAGCCTACCAAGAATCCAAGACACATTTTGTTTGTGTGAACACTTACTGAAACTTAAGAATA
  ATCACTGTGACCAACTTACAGTAAAACTTAAACAAATGGAAAATATGGTCAGTGTACTACAAAATGAGCT
  ATCTGAAACAAAAAAGACAAAATTACAGTTAGAACTTCAAAAAATTGAATGGGAGAAAGAGCTGTACGAT
  TTGAGACTTGCCTTAAAACAAGAAAATGAGGAGAAAAGAAATGCCGATATGTTGTATAATAAAGATAGTG
  AACAGTTAAGAATAAAAGAAGAGGAGTGTGGGAAAGTGGTTGAAACAAAGCAACAACTTAAATGGAATCT
  GAGAAGACTTGTTAAGGAATTGAGGACAGTAAGAAATAACTTGGATCTGGTTGTGCAGGAGAGAAACGAT
  GCCCAGAAGCAACTTTCTGAAGAACAGGATGCCAGAATATTACAAGATCAGATTCTGACGAGTAAACAAA
  AGGAACTAGAAATGGCTCGAAAGAAAATGAATTCTGAGATTTCTCATAGGCATCAGAAAGAAAAGGATCT
  CTTTCATGAAGATTGCATGTTGCAGGAAGAAATTGCCTTGCTGAGACTGGAAATAGATACAATAAAAAAT
  CAGAACAAGCAAAAGGAAAAGAAATATTTTGAGGACATTGAGGCTGTGAAAGAAAAGAATGATAACCTTC
  AAAAAATTATAAAACTAAATGAGGAAACATTAACAGAAACAATACTCCAGTACAGTGGACAGCTGAACAA
  TCTGACAGCTGAGAACAAAATACTCAATTCTGAACTGGAGAATGGGAAACAGAACCAAGAAAGACTAGAA
  ATAGAAATGGAATCATACCGTTGTAGACTAGCTGCTGCTGTACGTGACTGTGATCAAAGTCAGACAGCAA
  GAGACCTAAAACTTGATTTCCAGAGAACAAGACAAGAGTGGGTTCGTTTACATGACAAAATGAAGGTTGA
  TATGTCTGGCCTACAAGCTAAGAATGAGATTCTTTCTGAAAAACTTTCTAATGCTGAAAGTAAAATTAAC
  AGCCTACAAATTCAGCTCCATAACACAAGAGATGCTCTTGGAAGAGAGAGTTTGATTTTGGAACGTGTGC
  AAAGAGACCTCAGCCAAACACAGTGTCAGAAGAAAGAAACTGAACAAATGTACCAAATTGAACAAAGCAA
  ACTGAAGAAATACATTGCCAAGCAGGAATCTGTAGAGGAGAGATTATCTCAACTACAAAGTGAAAATATG
  TTGCTTCGACAGCAACTGGATGATGCTCACAAGAAAGCTAACAGTCAAGAAAAGACAAGCAGTACTATCC
  AAGACCAGTTTCATTCTGCTGCCAAAAATCTTCAAGCTGAGAGTGAAAAGCAGATTCTTTCACTACAAGA
  GAAGAACAAGGAGCTGATGGATGAATATAATCATTTAAAAGAAAGAATGGATCAATGTGAGAAAGAGAAA
  GCAGGAAGAAAAATTGACCTTACAGAAGCACAGGAAACTGTACCTTCACGATGTCTACATCTGGATGCAG
  AGAATGAAGTTCTTCAACTTCAACAGACATTATTCTCTATGAAAGCAATACAAAAGCAATGTGAAACACT
  ACAGAAGAATAAGAAGCAGCTGAAACAAGAAGTAGTAAACCTCAAAAGTTATATGGAAAGAAATATGTTA
  GAACGTGGTAAAGCTGAATGGCATAAACTGTTGATTGAAGAAAGAGCAAGGAAGGAGATAGAAGAAAAAT
  TAAACGAAGCCATTCTCACCTTGCAGAAACAAGCAGCAGTATCTCATGAACAGTTAGTACAGTTAAGGGA
  GGATAATACTACTTCAATAAAAACTCAGATGGAACTCACAATCAAAGATCTGGAATCTGAAATCTCCAGA
  ATAAAAACTTCGCAAGCCGACTTTAATAAAACCGAATTGGAAAGATATAAGGAACTCTACCTAGAAGAAG
  TGAAAGTTAGAGAATCCTTGTCAAATGAACTCAGTAGAACTAATGAGATGATAGCAGAGGTCAGTACGCA
  ACTTACTGTGGAGAAAGAGCAGACCAGATCCAGATCTCTATTCACTGCTTATGCTACAAGGCCAGTCCTA
  GAGTCACCTTGCGTTGGAAATCTTAATGATAGTGAAGGTCTCAACAGAAAACATATTCCAAGAAAAAAGA
  GGTCTGCTCTTAAGGACATGGAGAGCTACTTGTTGAAGATGCAGCAGAAGTTGCAAAATGATCTAACTGC
  AGAAGTAGCAGGATCCAGCCAGACTGGACTGCACAGAATTCCACAGTGCTCGTCCTTCTCCTCCAGCTCT
  TTGCACTTGCTTCTTTGCTCTATCTGCCAACCTTTTTTTCTCATCCTTCAGCTGCTACTAAATATGAACC
  TGGATCCTATA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC221755 representing NM_014695
  Red=Cloning site Green=Tags(s)
  
  MASWGGEKRGGAEGSPKPAVYATRKTPSVGSQGDQWYLGYPGDQWSSGFPYSWWKNSVGSESKHGEGALD
  QPQHDVRLEDLGELHRAARSGDVPGVEHILAPGDTGVDKRDRKKSIQQLVPEYKEKQTPESLPQNNNPDW
  HPTNLTLSDETCQRSKNLKVDDKCPSVSPSMPENQSATKELGQMNLTEREKMDTGVVLLSGNDTLHDLCQ
  SQLPENKESKEAEQDSELTSEEEQERLKGCENKQPQKTSQEPEMAKDCDREDIPIYPVLPHVQKSEEMWI
  EQGKLEWKNQLKLVINELKQRFGEIYEKYKIPACPEEEPLLDNSTRGTDVKDIPFNLTNNIPGCEEEDAS
  EISVSVVFETFPEQKEPSLKNIIHPYYHPYSGSQEHVCQSSSKFHLHENKLDCDNDNKPGIGHIFSTDKN
  FHNDASTKKARNPEVVMVEMKEDQEFDLQMTKNMNQNSDSGSTNNYKSLKPKLENLSSLPPDSDRTSEVY
  LHEELQQDMQKFKNEVNTLEEEFLALKKEDVQLHKDVEEEMEKHRSNSTELSGTLTDGTTVGNDDDGLNQ
  QIPRKENGEHDRPADKTSNEKNEVKNQIYPEADFADSMEPSEIASEDCELSHSVYENFMLLIEQLRMEYK
  DSASLPRIQDTFCLCEHLLKLKNNHCDQLTVKLKQMENMVSVLQNELSETKKTKLQLELQKIEWEKELYD
  LRLALKQENEEKRNADMLYNKDSEQLRIKEEECGKVVETKQQLKWNLRRLVKELRTVRNNLDLVVQERND
  AQKQLSEEQDARILQDQILTSKQKELEMARKKMNSEISHRHQKEKDLFHEDCMLQEEIALLRLEIDTIKN
  QNKQKEKKYFEDIEAVKEKNDNLQKIIKLNEETLTETILQYSGQLNNLTAENKILNSELENGKQNQERLE
  IEMESYRCRLAAAVRDCDQSQTARDLKLDFQRTRQEWVRLHDKMKVDMSGLQAKNEILSEKLSNAESKIN
  SLQIQLHNTRDALGRESLILERVQRDLSQTQCQKKETEQMYQIEQSKLKKYIAKQESVEERLSQLQSENM
  LLRQQLDDAHKKANSQEKTSSTIQDQFHSAAKNLQAESEKQILSLQEKNKELMDEYNHLKERMDQCEKEK
  AGRKIDLTEAQETVPSRCLHLDAENEVLQLQQTLFSMKAIQKQCETLQKNKKQLKQEVVNLKSYMERNML
  ERGKAEWHKLLIEERARKEIEEKLNEAILTLQKQAAVSHEQLVQLREDNTTSIKTQMELTIKDLESEISR
  IKTSQADFNKTELERYKELYLEEVKVRESLSNELSRTNEMIAEVSTQLTVEKEQTRSRSLFTAYATRPVL
  ESPCVGNLNDSEGLNRKHIPRKKRSALKDMESYLLKMQQKLQNDLTAEVAGSSQTGLHRIPQCSSFSSSS
  LHLLLCSICQPFFLILQLLLNMNLDPI
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_014695
• ORF Size: 4281 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_014695.1, NP_055510.1
• RefSeq Size: 5828 bp
• RefSeq ORF: 4284 bp
• Locus ID: 9720
• UniProt ID: A2RUR9
• Cytogenetics: 17p11.2
• MW: 165.1 kDa