FHOD3 (NM_025135) Human Tagged Lenti ORF Clone
CAT#: RC221215L3
- LentiORF®
Lenti ORF clone of Human formin homology 2 domain containing 3 (FHOD3), Myc-DDK-tagged
"NM_025135" in other vectors (5)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | FHOD3 |
Synonyms | CMH28; FHOS2; Formactin2 |
Vector | pLenti-C-Myc-DDK-P2A-Puro |
E. coli Selection | Chloramphenicol (34 ug/mL) |
Mammalian Cell Selection | Puromycin |
Sequence Data |
The ORF insert of this clone is exactly the same as(RC221215).
|
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_025135 |
ORF Size | 4317 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_025135.2 |
RefSeq Size | 4942 bp |
RefSeq ORF | 4320 bp |
Locus ID | 80206 |
UniProt ID | Q2V2M9 |
Cytogenetics | 18q12.2 |
Domains | FH2 |
MW | 160.6 kDa |
Gene Summary | The protein encoded by this gene is a member of the diaphanous-related formins (DRF), and contains multiple domains, including GBD (GTPase-binding domain), DID (diaphanous inhibitory domain), FH1 (formin homology 1), FH2 (formin homology 2), and DAD (diaphanous auto-regulatory domain) domains. This protein is thought to play a role in actin filament polymerization in cardiomyocytes. Mutations in this gene have been associated with dilated cardiomyopathy (DCM), characterized by dilation of the ventricular chamber, leading to impairment of systolic pump function and subsequent heart failure. Increased levels of the protein encoded by this gene have been observed in individuals with hypertrophic cardiomyopathy (HCM). Alternative splicing results in multiple transcript variants encoding different isoforms. A muscle-specific isoform has been shown to possess a casein kinase 2 (CK2) phosphorylation site at the C-terminal end of the FH2 domain. Phosphorylation of this site alters its interaction with sequestosome 1 (SQSTM1), and targets this isoform to myofibrils, while other isoforms form cytoplasmic aggregates. [provided by RefSeq, Aug 2015] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC221215 | FHOD3 (Myc-DDK-tagged)-Human formin homology 2 domain containing 3 (FHOD3) |
USD 1,231.00 |
|
RC221215L4 | Lenti ORF clone of Human formin homology 2 domain containing 3 (FHOD3), mGFP tagged |
USD 1,531.00 |
|
RG221215 | FHOD3 (tGFP-tagged) - Human formin homology 2 domain containing 3 (FHOD3) |
USD 1,431.00 |
|
SC110946 | FHOD3 (untagged)-Human formin homology 2 domain containing 3 (FHOD3) |
USD 457.00 |
|
SC318619 | FHOD3 (untagged)-Human formin homology 2 domain containing 3 (FHOD3) |
USD 1,355.00 |
{0} Product Review(s)
Be the first one to submit a review