HKDC1 (NM_025130) Human Tagged ORF Clone

CAT#: RC221178

HKDC1 (Myc-DDK-tagged)-Human hexokinase domain containing 1 (HKDC1)

Product Images

Circular map for RC221178

• 

  Western blot validation of overexpression lysate (Cat# LY410883) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC221178 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified HKDC1 protein (Cat# TP321178). The protein was produced from HEK293T cells transfected with HKDC1 cDNA clone (Cat# RC221178) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: HKDC1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC221178 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTTGCGGTCCACTTGATGGCATTTTACTTCAGCAAGCTGAAGGAGGACCAGATCAAGAAGGTGGACA
  GGTTCCTGTATCACATGCGGCTCTCCGATGACACCCTTTTGGACATCATGAGGCGGTTCCGGGCTGAGAT
  GGAGAAGGGCCTGGCAAAGGACACCAACCCCACGGCTGCAGTGAAGATGTTGCCCACCTTCGTCAGGGCC
  ATTCCCGATGGTTCCGAAAATGGGGAGTTCCTTTCCCTGGATCTCGGAGGGTCCAAGTTCCGAGTGCTGA
  AGGTGCAAGTCGCTGAAGAGGGGAAGCGACACGTGCAGATGGAGAGTCAGTTCTACCCAACGCCCAATGA
  AATCATCCGCGGGAACGGCACAGAGCTGTTTGAATATGTAGCTGACTGTCTGGCAGATTTCATGAAGACC
  AAAGATTTAAAGCATAAGAAATTGCCCCTTGGCCTAACTTTTTCTTTCCCCTGTCGACAGACTAAACTGG
  AAGAGGGTGTCCTACTTTCGTGGACAAAAAAGTTTAAGGCACGAGGAGTTCAGGACACGGATGTGGTGAG
  CCGTCTGACCAAAGCCATGAGAAGACACAAGGACATGGACGTGGACATCCTGGCCCTGGTCAATGACACC
  GTGGGGACCATGATGACCTGTGCCTATGACGACCCCTACTGCGAAGTTGGTGTCATCATCGGAACTGGCA
  CCAATGCGTGTTACATGGAGGACATGAGCAACATTGACCTGGTGGAGGGCGACGAGGGCAGGATGTGCAT
  CAACACAGAGTGGGGGGCCTTCGGGGACGACGGGGCCCTGGAGGACATTCGCACTGAGTTCGACAGGGAG
  CTGGACCTCGGCTCTCTCAACCCAGGAAAGCAACTGTTCGAGAAGATGATCAGTGGCCTGTACCTGGGGG
  AGCTTGTCAGGCTTATCTTGCTGAAGATGGCCAAGGCTGGCCTCCTGTTTGGTGGTGAGAAATCTTCTGC
  TCTCCACACTAAGGGCAAGATCGAAACACGGCACGTGGCTGCCATGGAGAAGTATAAAGAAGGCCTTGCT
  AATACAAGAGAGATCCTGGTGGACCTGGGTCTGGAACCGTCTGAGGCTGACTGCATTGCCGTCCAGCATG
  TCTGTACCATCGTCTCCTTCCGCTCGGCCAATCTCTGTGCAGCAGCTCTGGCGGCCATCCTGACACGCCT
  CCGGGAGAACAAGAAGGTGGAACGGCTCCGGACCACAGTGGGCATGGACGGCACCCTCTACAAGATACAC
  CCTCAGTACCCAAAACGCCTGCACAAGGTGGTGAGGAAACTGGTCCCAAGCTGTGATGTCCGCTTCCTCC
  TGTCAGAGAGTGGCAGCACCAAGGGGGCCGCCATGGTGACCGCGGTGGCCTCCCGCGTGCAGGCCCAGCG
  GAAGCAGATCGACAGGGTGCTGGCTTTGTTCCAGCTGACCCGAGAGCAGCTCGTGGACGTGCAGGCCAAG
  ATGCGGGCTGAGCTGGAGTATGGGCTGAAGAAGAAGAGCCACGGGCTGGCCACGGTCAGGATGCTGCCCA
  CCTACGTCTGCGGGCTGCCGGACGGCACAGAGAAAGGAAAGTTTCTCGCCCTGGATCTTGGGGGAACCAA
  CTTCCGGGTCCTCCTGGTGAAGATCAGAAGTGGACGGAGGTCAGTGCGAATGTACAACAAGATCTTCGCC
  ATCCCCCTGGAGATCATGCAGGGCACTGGTGAGGAGCTCTTTGATCACATTGTGCAGTGCATCGCCGACT
  TCCTGGACTACATGGGCCTCAAGGGAGCCTCCCTACCTTTGGGCTTCACATTCTCATTTCCCTGCAGGCA
  GATGAGCATTGACAAGGGAACACTCATAGGGTGGACCAAAGGTTTCAAGGCCACTGACTGTGAAGGGGAG
  GACGTGGTGGACATGCTCAGGGAAGCCATCAAGAGGAGAAACGAGTTTGACCTGGACATTGTTGCAGTCG
  TGAATGATACAGTGGGGACCATGATGACCTGTGGCTATGAAGATCCTAATTGTGAGATTGGCCTGATTGC
  AGGAACAGGCAGCAACATGTGCTACATGGAGGACATGAGGAACATCGAGATGGTGGAGGGGGGTGAAGGG
  AAGATGTGCATCAATACAGAGTGGGGAGGATTTGGAGACAATGGCTGCATAGATGACATCCGGACCCGAT
  ACGACACGGAGGTGGATGAGGGGTCCTTGAATCCTGGCAAGCAGAGATACGAGAAAATGACCAGTGGGAT
  GTACTTGGGGGAGATTGTGCGGCAGATCCTGATCGACCTGACCAAGCAGGGTCTCCTCTTCCGAGGGCAG
  ATTTCAGAGCGTCTCCGGACCAGGGGCATCTTCGAAACCAAGTTCCTGTCCCAGATCGAAAGCGATCGGC
  TGGCCCTTCTCCAGGTCAGGAGGATTCTGCAGCAGCTGGGCCTGGACAGCACGTGTGAGGACAGCATCGT
  GGTGAAGGAGGTGTGCGGAGCCGTGTCCCGGCGGGCGGCCCAGCTCTGCGGTGCTGGCCTGGCCGCTATA
  GTGGAAAAAAGGAGAGAAGACCAGGGGCTAGAGCACCTGAGGATCACTGTGGGTGTGGACGGCACCCTGT
  ACAAGCTGCACCCTCACTTTTCTAGAATATTGCAGGAAACTGTGAAGGAACTAGCCCCTCGATGTGATGT
  GACATTCATGCTGTCAGAAGATGGCAGTGGAAAAGGGGCAGCACTGATCACTGCTGTGGCCAAGAGGTTA
  CAGCAGGCACAGAAGGAGAAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC221178 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MFAVHLMAFYFSKLKEDQIKKVDRFLYHMRLSDDTLLDIMRRFRAEMEKGLAKDTNPTAAVKMLPTFVRA
  IPDGSENGEFLSLDLGGSKFRVLKVQVAEEGKRHVQMESQFYPTPNEIIRGNGTELFEYVADCLADFMKT
  KDLKHKKLPLGLTFSFPCRQTKLEEGVLLSWTKKFKARGVQDTDVVSRLTKAMRRHKDMDVDILALVNDT
  VGTMMTCAYDDPYCEVGVIIGTGTNACYMEDMSNIDLVEGDEGRMCINTEWGAFGDDGALEDIRTEFDRE
  LDLGSLNPGKQLFEKMISGLYLGELVRLILLKMAKAGLLFGGEKSSALHTKGKIETRHVAAMEKYKEGLA
  NTREILVDLGLEPSEADCIAVQHVCTIVSFRSANLCAAALAAILTRLRENKKVERLRTTVGMDGTLYKIH
  PQYPKRLHKVVRKLVPSCDVRFLLSESGSTKGAAMVTAVASRVQAQRKQIDRVLALFQLTREQLVDVQAK
  MRAELEYGLKKKSHGLATVRMLPTYVCGLPDGTEKGKFLALDLGGTNFRVLLVKIRSGRRSVRMYNKIFA
  IPLEIMQGTGEELFDHIVQCIADFLDYMGLKGASLPLGFTFSFPCRQMSIDKGTLIGWTKGFKATDCEGE
  DVVDMLREAIKRRNEFDLDIVAVVNDTVGTMMTCGYEDPNCEIGLIAGTGSNMCYMEDMRNIEMVEGGEG
  KMCINTEWGGFGDNGCIDDIRTRYDTEVDEGSLNPGKQRYEKMTSGMYLGEIVRQILIDLTKQGLLFRGQ
  ISERLRTRGIFETKFLSQIESDRLALLQVRRILQQLGLDSTCEDSIVVKEVCGAVSRRAAQLCGAGLAAI
  VEKRREDQGLEHLRITVGVDGTLYKLHPHFSRILQETVKELAPRCDVTFMLSEDGSGKGAALITAVAKRL
  QQAQKEN
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_025130
• ORF Size: 2751 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_025130.3, NP_079406.3
• RefSeq Size: 3719 bp
• RefSeq ORF: 2754 bp
• Locus ID: 80201
• UniProt ID: Q2TB90
• Cytogenetics: 10q22.1
• Domains: hexokinase
• Protein Families: Druggable Genome
• MW: 102.5 kDa
• Gene Summary: This gene encodes a member of the hexokinase protein family. The encoded protein is involved in glucose metabolism, and reduced expression may be associated with gestational diabetes mellitus. High expression of this gene may also be associated with poor prognosis in hepatocarcinoma. [provided by RefSeq, Sep 2016]