HKDC1 (NM_025130) Human Tagged ORF Clone

CAT#: RC221178

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HKDC1 (Myc-DDK-tagged)-Human hexokinase domain containing 1 (HKDC1)



  "NM_025130" in other vectors (6)

Reconstitution Protocol

USD 1,259.00

In Stock*

Size
    • 10 ug

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Frequently bought together (4)
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Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
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Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol HKDC1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC221178 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTTTGCGGTCCACTTGATGGCATTTTACTTCAGCAAGCTGAAGGAGGACCAGATCAAGAAGGTGGACA
GGTTCCTGTATCACATGCGGCTCTCCGATGACACCCTTTTGGACATCATGAGGCGGTTCCGGGCTGAGAT
GGAGAAGGGCCTGGCAAAGGACACCAACCCCACGGCTGCAGTGAAGATGTTGCCCACCTTCGTCAGGGCC
ATTCCCGATGGTTCCGAAAATGGGGAGTTCCTTTCCCTGGATCTCGGAGGGTCCAAGTTCCGAGTGCTGA
AGGTGCAAGTCGCTGAAGAGGGGAAGCGACACGTGCAGATGGAGAGTCAGTTCTACCCAACGCCCAATGA
AATCATCCGCGGGAACGGCACAGAGCTGTTTGAATATGTAGCTGACTGTCTGGCAGATTTCATGAAGACC
AAAGATTTAAAGCATAAGAAATTGCCCCTTGGCCTAACTTTTTCTTTCCCCTGTCGACAGACTAAACTGG
AAGAGGGTGTCCTACTTTCGTGGACAAAAAAGTTTAAGGCACGAGGAGTTCAGGACACGGATGTGGTGAG
CCGTCTGACCAAAGCCATGAGAAGACACAAGGACATGGACGTGGACATCCTGGCCCTGGTCAATGACACC
GTGGGGACCATGATGACCTGTGCCTATGACGACCCCTACTGCGAAGTTGGTGTCATCATCGGAACTGGCA
CCAATGCGTGTTACATGGAGGACATGAGCAACATTGACCTGGTGGAGGGCGACGAGGGCAGGATGTGCAT
CAACACAGAGTGGGGGGCCTTCGGGGACGACGGGGCCCTGGAGGACATTCGCACTGAGTTCGACAGGGAG
CTGGACCTCGGCTCTCTCAACCCAGGAAAGCAACTGTTCGAGAAGATGATCAGTGGCCTGTACCTGGGGG
AGCTTGTCAGGCTTATCTTGCTGAAGATGGCCAAGGCTGGCCTCCTGTTTGGTGGTGAGAAATCTTCTGC
TCTCCACACTAAGGGCAAGATCGAAACACGGCACGTGGCTGCCATGGAGAAGTATAAAGAAGGCCTTGCT
AATACAAGAGAGATCCTGGTGGACCTGGGTCTGGAACCGTCTGAGGCTGACTGCATTGCCGTCCAGCATG
TCTGTACCATCGTCTCCTTCCGCTCGGCCAATCTCTGTGCAGCAGCTCTGGCGGCCATCCTGACACGCCT
CCGGGAGAACAAGAAGGTGGAACGGCTCCGGACCACAGTGGGCATGGACGGCACCCTCTACAAGATACAC
CCTCAGTACCCAAAACGCCTGCACAAGGTGGTGAGGAAACTGGTCCCAAGCTGTGATGTCCGCTTCCTCC
TGTCAGAGAGTGGCAGCACCAAGGGGGCCGCCATGGTGACCGCGGTGGCCTCCCGCGTGCAGGCCCAGCG
GAAGCAGATCGACAGGGTGCTGGCTTTGTTCCAGCTGACCCGAGAGCAGCTCGTGGACGTGCAGGCCAAG
ATGCGGGCTGAGCTGGAGTATGGGCTGAAGAAGAAGAGCCACGGGCTGGCCACGGTCAGGATGCTGCCCA
CCTACGTCTGCGGGCTGCCGGACGGCACAGAGAAAGGAAAGTTTCTCGCCCTGGATCTTGGGGGAACCAA
CTTCCGGGTCCTCCTGGTGAAGATCAGAAGTGGACGGAGGTCAGTGCGAATGTACAACAAGATCTTCGCC
ATCCCCCTGGAGATCATGCAGGGCACTGGTGAGGAGCTCTTTGATCACATTGTGCAGTGCATCGCCGACT
TCCTGGACTACATGGGCCTCAAGGGAGCCTCCCTACCTTTGGGCTTCACATTCTCATTTCCCTGCAGGCA
GATGAGCATTGACAAGGGAACACTCATAGGGTGGACCAAAGGTTTCAAGGCCACTGACTGTGAAGGGGAG
GACGTGGTGGACATGCTCAGGGAAGCCATCAAGAGGAGAAACGAGTTTGACCTGGACATTGTTGCAGTCG
TGAATGATACAGTGGGGACCATGATGACCTGTGGCTATGAAGATCCTAATTGTGAGATTGGCCTGATTGC
AGGAACAGGCAGCAACATGTGCTACATGGAGGACATGAGGAACATCGAGATGGTGGAGGGGGGTGAAGGG
AAGATGTGCATCAATACAGAGTGGGGAGGATTTGGAGACAATGGCTGCATAGATGACATCCGGACCCGAT
ACGACACGGAGGTGGATGAGGGGTCCTTGAATCCTGGCAAGCAGAGATACGAGAAAATGACCAGTGGGAT
GTACTTGGGGGAGATTGTGCGGCAGATCCTGATCGACCTGACCAAGCAGGGTCTCCTCTTCCGAGGGCAG
ATTTCAGAGCGTCTCCGGACCAGGGGCATCTTCGAAACCAAGTTCCTGTCCCAGATCGAAAGCGATCGGC
TGGCCCTTCTCCAGGTCAGGAGGATTCTGCAGCAGCTGGGCCTGGACAGCACGTGTGAGGACAGCATCGT
GGTGAAGGAGGTGTGCGGAGCCGTGTCCCGGCGGGCGGCCCAGCTCTGCGGTGCTGGCCTGGCCGCTATA
GTGGAAAAAAGGAGAGAAGACCAGGGGCTAGAGCACCTGAGGATCACTGTGGGTGTGGACGGCACCCTGT
ACAAGCTGCACCCTCACTTTTCTAGAATATTGCAGGAAACTGTGAAGGAACTAGCCCCTCGATGTGATGT
GACATTCATGCTGTCAGAAGATGGCAGTGGAAAAGGGGCAGCACTGATCACTGCTGTGGCCAAGAGGTTA
CAGCAGGCACAGAAGGAGAAC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC221178 protein sequence
Red=Cloning site Green=Tags(s)

MFAVHLMAFYFSKLKEDQIKKVDRFLYHMRLSDDTLLDIMRRFRAEMEKGLAKDTNPTAAVKMLPTFVRA
IPDGSENGEFLSLDLGGSKFRVLKVQVAEEGKRHVQMESQFYPTPNEIIRGNGTELFEYVADCLADFMKT
KDLKHKKLPLGLTFSFPCRQTKLEEGVLLSWTKKFKARGVQDTDVVSRLTKAMRRHKDMDVDILALVNDT
VGTMMTCAYDDPYCEVGVIIGTGTNACYMEDMSNIDLVEGDEGRMCINTEWGAFGDDGALEDIRTEFDRE
LDLGSLNPGKQLFEKMISGLYLGELVRLILLKMAKAGLLFGGEKSSALHTKGKIETRHVAAMEKYKEGLA
NTREILVDLGLEPSEADCIAVQHVCTIVSFRSANLCAAALAAILTRLRENKKVERLRTTVGMDGTLYKIH
PQYPKRLHKVVRKLVPSCDVRFLLSESGSTKGAAMVTAVASRVQAQRKQIDRVLALFQLTREQLVDVQAK
MRAELEYGLKKKSHGLATVRMLPTYVCGLPDGTEKGKFLALDLGGTNFRVLLVKIRSGRRSVRMYNKIFA
IPLEIMQGTGEELFDHIVQCIADFLDYMGLKGASLPLGFTFSFPCRQMSIDKGTLIGWTKGFKATDCEGE
DVVDMLREAIKRRNEFDLDIVAVVNDTVGTMMTCGYEDPNCEIGLIAGTGSNMCYMEDMRNIEMVEGGEG
KMCINTEWGGFGDNGCIDDIRTRYDTEVDEGSLNPGKQRYEKMTSGMYLGEIVRQILIDLTKQGLLFRGQ
ISERLRTRGIFETKFLSQIESDRLALLQVRRILQQLGLDSTCEDSIVVKEVCGAVSRRAAQLCGAGLAAI
VEKRREDQGLEHLRITVGVDGTLYKLHPHFSRILQETVKELAPRCDVTFMLSEDGSGKGAALITAVAKRL
QQAQKEN

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_025130
ORF Size 2751 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_025130.3, NP_079406.3
RefSeq Size 3719 bp
RefSeq ORF 2754 bp
Locus ID 80201
UniProt ID Q2TB90
Cytogenetics 10q22.1
Domains hexokinase
Protein Families Druggable Genome
MW 102.5 kDa
Gene Summary This gene encodes a member of the hexokinase protein family. The encoded protein is involved in glucose metabolism, and reduced expression may be associated with gestational diabetes mellitus. High expression of this gene may also be associated with poor prognosis in hepatocarcinoma. [provided by RefSeq, Sep 2016]

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