HKDC1 (NM_025130) Human Untagged Clone

CAT#: SC314919

HKDC1 (untagged)-Human hexokinase domain containing 1 (HKDC1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: HKDC1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC314919 representing NM_025130.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGTTTGCGGTCCACTTGATGGCATTTTACTTCAGCAAGCTGAAGGAGGACCAGATCAAGAAGGTGGAC
  AGGTTCCTGTATCACATGCGGCTCTCCGATGACACCCTTTTGGACATCATGAGGCGGTTCCGGGCTGAG
  ATGGAGAAGGGCCTGGCAAAGGACACCAACCCCACGGCTGCAGTGAAGATGTTGCCCACCTTCGTCAGG
  GCCATTCCCGATGGTTCCGAAAATGGGGAGTTCCTTTCCCTGGATCTCGGAGGGTCCAAGTTCCGAGTG
  CTGAAGGTGCAAGTCGCTGAAGAGGGGAAGCGACACGTGCAGATGGAGAGTCAGTTCTACCCAACGCCC
  AATGAAATCATCCGCGGGAACGGCACAGAGCTGTTTGAATATGTAGCTGACTGTCTGGCAGATTTCATG
  AAGACCAAAGATTTAAAGCATAAGAAATTGCCCCTTGGCCTAACTTTTTCTTTCCCCTGTCGACAGACT
  AAACTGGAAGAGGGTGTCCTACTTTCGTGGACAAAAAAGTTTAAGGCACGAGGAGTTCAGGACACGGAT
  GTGGTGAGCCGTCTGACCAAAGCCATGAGAAGACACAAGGACATGGACGTGGACATCCTGGCCCTGGTC
  AATGACACCGTGGGGACCATGATGACCTGTGCCTATGACGACCCCTACTGCGAAGTTGGTGTCATCATC
  GGAACTGGCACCAATGCGTGTTACATGGAGGACATGAGCAACATTGACCTGGTGGAGGGCGACGAGGGC
  AGGATGTGCATCAACACAGAGTGGGGGGCCTTCGGGGACGACGGGGCCCTGGAGGACATTCGCACTGAG
  TTCGACAGGGAGCTGGACCTCGGCTCTCTCAACCCAGGAAAGCAACTGTTCGAGAAGATGATCAGTGGC
  CTGTACCTGGGGGAGCTTGTCAGGCTTATCTTGCTGAAGATGGCCAAGGCTGGCCTCCTGTTTGGTGGT
  GAGAAATCTTCTGCTCTCCACACTAAGGGCAAGATCGAAACACGGCACGTGGCTGCCATGGAGAAGTAT
  AAAGAAGGCCTTGCTAATACAAGAGAGATCCTGGTGGACCTGGGTCTGGAACCGTCTGAGGCTGACTGC
  ATTGCCGTCCAGCATGTCTGTACCATCGTCTCCTTCCGCTCGGCCAATCTCTGTGCAGCAGCTCTGGCG
  GCCATCCTGACACGCCTCCGGGAGAACAAGAAGGTGGAACGGCTCCGGACCACAGTGGGCATGGACGGC
  ACCCTCTACAAGATACACCCTCAGTACCCAAAACGCCTGCACAAGGTGGTGAGGAAACTGGTCCCAAGC
  TGTGATGTCCGCTTCCTCCTGTCAGAGAGTGGCAGCACCAAGGGGGCCGCCATGGTGACCGCGGTGGCC
  TCCCGCGTGCAGGCCCAGCGGAAGCAGATCGACAGGGTGCTGGCTTTGTTCCAGCTGACCCGAGAGCAG
  CTCGTGGACGTGCAGGCCAAGATGCGGGCTGAGCTGGAGTATGGGCTGAAGAAGAAGAGCCACGGGCTG
  GCCACGGTCAGGATGCTGCCCACCTACGTCTGCGGGCTGCCGGACGGCACAGAGAAAGGAAAGTTTCTC
  GCCCTGGATCTTGGGGGAACCAACTTCCGGGTCCTCCTGGTGAAGATCAGAAGTGGACGGAGGTCAGTG
  CGAATGTACAACAAGATCTTCGCCATCCCCCTGGAGATCATGCAGGGCACTGGTGAGGAGCTCTTTGAT
  CACATTGTGCAGTGCATCGCCGACTTCCTGGACTACATGGGCCTCAAGGGAGCCTCCCTACCTTTGGGC
  TTCACATTCTCATTTCCCTGCAGGCAGATGAGCATTGACAAGGGAACACTCATAGGGTGGACCAAAGGT
  TTCAAGGCCACTGACTGTGAAGGGGAGGACGTGGTGGACATGCTCAGGGAAGCCATCAAGAGGAGAAAC
  GAGTTTGACCTGGACATTGTTGCAGTCGTGAATGATACAGTGGGGACCATGATGACCTGTGGCTATGAA
  GATCCTAATTGTGAGATTGGCCTGATTGCAGGAACAGGCAGCAACATGTGCTACATGGAGGACATGAGG
  AACATCGAGATGGTGGAGGGGGGTGAAGGGAAGATGTGCATCAATACAGAGTGGGGAGGATTTGGAGAC
  AATGGCTGCATAGATGACATCCGGACCCGATACGACACGGAGGTGGATGAGGGGTCCTTGAATCCTGGC
  AAGCAGAGATACGAGAAAATGACCAGTGGGATGTACTTGGGGGAGATTGTGCGGCAGATCCTGATCGAC
  CTGACCAAGCAGGGTCTCCTCTTCCGAGGGCAGATTTCAGAGCGTCTCCGGACCAGGGGCATCTTCGAA
  ACCAAGTTCCTGTCCCAGATCGAAAGCGATCGGCTGGCCCTTCTCCAGGTCAGGAGGATTCTGCAGCAG
  CTGGGCCTGGACAGCACGTGTGAGGACAGCATCGTGGTGAAGGAGGTGTGCGGAGCCGTGTCCCGGCGG
  GCGGCCCAGCTCTGCGGTGCTGGCCTGGCCGCTATAGTGGAAAAAAGGAGAGAAGACCAGGGGCTAGAG
  CACCTGAGGATCACTGTGGGTGTGGACGGCACCCTGTACAAGCTGCACCCTCACTTTTCTAGAATATTG
  CAGGAAACTGTGAAGGAACTAGCCCCTCGATGTGATGTGACATTCATGCTGTCAGAAGATGGCAGTGGA
  AAAGGGGCAGCACTGATCACTGCTGTGGCCAAGAGGTTACAGCAGGCACAGAAGGAGAACTAG
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_025130
• Insert Size: 2754 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_025130.3
• RefSeq Size: 3719 bp
• RefSeq ORF: 2754 bp
• Locus ID: 80201
• UniProt ID: Q2TB90
• Cytogenetics: 10q22.1
• Domains: hexokinase
• Protein Families: Druggable Genome
• MW: 102.5 kDa
• Gene Summary: This gene encodes a member of the hexokinase protein family. The encoded protein is involved in glucose metabolism, and reduced expression may be associated with gestational diabetes mellitus. High expression of this gene may also be associated with poor prognosis in hepatocarcinoma. [provided by RefSeq, Sep 2016]