p90 Autoantigen (KIAA1524) (NM_020890) Human Tagged ORF Clone

CAT#: RC219918

KIAA1524 (Myc-DDK-tagged)-Human KIAA1524 (KIAA1524)

Product Images

Circular map for RC219918

• 

  HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY KIAA1524 (Cat# RC219918, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-KIAA1524 (Cat# TA808455)(1:2000). Positive lysates LY412250 (100ug) and LC412250 (20ug) can be purchased separately from OriGene.

• 

  Western blot validation of overexpression lysate (Cat# LY412250) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC219918 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified CIP2A protein (Cat# TP319918). The protein was produced from HEK293T cells transfected with CIP2A cDNA clone (Cat# RC219918) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: p90 Autoantigen
• Synonyms: KIAA1524; p90
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC219918 representing NM_020890
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACTCCACTGCCTGCTTGAAGTCCTTGCTCCTGACTGTCAGTCAGTACAAAGCCGTGAAGTCAGAGG
  CGAACGCCACTCAGCTTTTGCGGCACTTGGAGGTAATTTCTGGACAGAAACTCACACGACTATTTACATC
  AAATCAGATATTAACAAGTGAATGCTTGAGTTGCCTTGTAGAGCTACTTGAAGACCCCAACATAAGTGCT
  TCACTGATCTTAAGTATTATCGGTTTGCTGTCTCAACTAGCAGTAGACATTGAAACCAGAGATTGTCTTC
  AGAATACATATAATCTGAATAGTGTGCTGGCGGGAGTGGTTTGTCGGAGCAGCCACACTGATTCGGTGTT
  TTTGCAGTGCATTCAACTTCTACAGAAGTTAACATATAATGTCAAAATTTTCTATTCTGGTGCCAATATA
  GATGAATTAATTACGTTCCTGATAGATCACATTCAATCTTCTGAAGATGAGTTAAAAATGCCTTGTCTAG
  GATTATTGGCAAATCTTTGTCGGCACAATCTTTCTGTTCAAACGCACATAAAGACATTGAGTAATGTGAA
  ATCTTTTTATCGAACTCTTATCACCTTGTTGGCCCATAGTAGTTTAACTGTGGTTGTGTTTGCACTTTCA
  ATATTATCCAGTTTGACATTAAATGAAGAGGTGGGGGAAAAGCTATTCCATGCTCGAAACATTCATCAGA
  CTTTTCAACTAATATTTAATATTCTCATAAACGGTGATGGCACTCTAACTAGAAAGTATTCAGTTGACCT
  ACTGATGGATCTCCTTAAGAATCCTAAAATTGCTGATTATCTCACCAGATATGAGCACTTTTCTTCATGT
  CTTCACCAAGTATTAGGTCTTCTTAATGGAAAGGATCCTGATTCCTCTTCAAAGGTTTTAGAATTACTTC
  TTGCCTTCTGTTCAGTGACTCAGCTGCGCCATATGCTCACTCAGATGATGTTTGAACAGTCTCCACCTGG
  CAGCGCCACTCTGGGAAGCCATACTAAATGTTTAGAACCTACTGTGGCTCTACTGCGCTGGTTAAGCCAA
  CCTTTGGACGGATCAGAAAACTGTTCTGTTTTAGCATTGGAGTTGTTCAAGGAAATATTTGAGGATGTCA
  TAGATGCTGCTAACTGTTCCTCGGCTGATCGTTTTGTGACCCTTCTGCTGCCTACAATCCTTGATCAACT
  TCAGTTCACAGAACAAAATCTAGATGAGGCTTTAACAAGAAAAAAATGTGAAAGGATTGCCAAGGCCATT
  GAAGTTTTGTTAACTCTCTGTGGAGATGATACACTAAAAATGCATATTGCAAAAATCTTGACAACTGTCA
  AGTGTACCACTCTTATAGAACAACAATTTACATATGGCAAGATTGACCTGGGATTTGGAACAAAGGTTGC
  AGATTCTGAATTATGCAAACTTGCTGCTGATGTAATTTTGAAAACTCTTGATTTGATTAACAAACTTAAA
  CCATTGGTTCCTGGTATGGAAGTAAGCTTCTACAAAATACTTCAGGACCCACGTTTGATTACTCCTTTGG
  CTTTTGCTTTAACGTCAGATAATAGAGAACAAGTACAGTCTGGACTGAGAATATTATTGGAGGCTGCTCC
  ACTGCCAGATTTTCCTGCTTTAGTACTTGGAGAAAGTATAGCAGCAAACAATGCCTATAGACAACAGGAA
  ACAGAACATATACCCAGAAAAATGCCCTGGCAATCATCAAATCACAGTTTTCCAACATCAATAAAGTGTT
  TAACTCCTCATTTGAAAGATGGTGTTCCTGGATTGAATATTGAAGAATTAATAGAGAAACTTCAGTCTGG
  AATGGTGGTAAAGGATCAGATTTGTGATGTGAGAATATCTGACATAATGGATGTATATGAAATGAAACTA
  TCCACATTAGCTTCCAAAGAAAGCAGGCTACAAGATCTTTTGGAAACAAAAGCTCTAGCCCTTGCACAGG
  CTGATAGACTGATTGCTCAGCATCGCTGTCAAAGAACTCAAGCTGAAACAGAGGCACGGACACTTGCTAG
  TATGTTGAGAGAAGTTGAGAGAAAAAATGAAGAGCTTAGTGTGTTGCTGAAGGCGCAGCAAGTTGAATCA
  GAAAGAGCGCAGAGTGATATTGAGCATCTCTTTCAACATAATAGGAAGTTAGAGTCTGTGGCTGAAGAAC
  ATGAAATACTGACAAAATCCTACATGGAACTTCTTCAGAGAAATGAAAGTACTGAAAAGAAGAATAAAGA
  TTTACAGATCACATGTGATTCTCTGAATAAACAAATTGAGACAGTGAAAAAGTTGAATGAGTCACTCAAG
  GAACAAAATGAAAAAAGTATTGCCCAATTAATAGAGAAAGAAGAACAGAGAAAAGAAGTACAGAATCAGC
  TAGTAGACAGAGAACATAAGCTAGCAAATTTGCATCAAAAAACAAAAGTACAAGAAGAAAAGATTAAAAC
  CTTACAAAAGGAAAGGGAAGATAAGGAAGAAACCATTGATATCCTTAGAAAAGAATTAAGCAGAACAGAA
  CAGATAAGAAAAGAGTTGAGCATTAAGGCTTCCTCCCTAGAGGTTCAAAAGGCACAATTAGAAGGTCGTT
  TGGAAGAGAAAGAGTCCTTGGTGAAACTTCAGCAAGAGGAATTGAACAAACACTCCCACATGATAGCAAT
  GATCCACAGTTTAAGTGGTGGAAAAATAAATCCAGAAACTGTGAATCTCAGTATA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC219918 representing NM_020890
  Red=Cloning site Green=Tags(s)
  
  MDSTACLKSLLLTVSQYKAVKSEANATQLLRHLEVISGQKLTRLFTSNQILTSECLSCLVELLEDPNISA
  SLILSIIGLLSQLAVDIETRDCLQNTYNLNSVLAGVVCRSSHTDSVFLQCIQLLQKLTYNVKIFYSGANI
  DELITFLIDHIQSSEDELKMPCLGLLANLCRHNLSVQTHIKTLSNVKSFYRTLITLLAHSSLTVVVFALS
  ILSSLTLNEEVGEKLFHARNIHQTFQLIFNILINGDGTLTRKYSVDLLMDLLKNPKIADYLTRYEHFSSC
  LHQVLGLLNGKDPDSSSKVLELLLAFCSVTQLRHMLTQMMFEQSPPGSATLGSHTKCLEPTVALLRWLSQ
  PLDGSENCSVLALELFKEIFEDVIDAANCSSADRFVTLLLPTILDQLQFTEQNLDEALTRKKCERIAKAI
  EVLLTLCGDDTLKMHIAKILTTVKCTTLIEQQFTYGKIDLGFGTKVADSELCKLAADVILKTLDLINKLK
  PLVPGMEVSFYKILQDPRLITPLAFALTSDNREQVQSGLRILLEAAPLPDFPALVLGESIAANNAYRQQE
  TEHIPRKMPWQSSNHSFPTSIKCLTPHLKDGVPGLNIEELIEKLQSGMVVKDQICDVRISDIMDVYEMKL
  STLASKESRLQDLLETKALALAQADRLIAQHRCQRTQAETEARTLASMLREVERKNEELSVLLKAQQVES
  ERAQSDIEHLFQHNRKLESVAEEHEILTKSYMELLQRNESTEKKNKDLQITCDSLNKQIETVKKLNESLK
  EQNEKSIAQLIEKEEQRKEVQNQLVDREHKLANLHQKTKVQEEKIKTLQKEREDKEETIDILRKELSRTE
  QIRKELSIKASSLEVQKAQLEGRLEEKESLVKLQQEELNKHSHMIAMIHSLSGGKINPETVNLSI
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_020890
• ORF Size: 2715 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_020890.3
• RefSeq Size: 3395 bp
• RefSeq ORF: 2718 bp
• Locus ID: 57650
• UniProt ID: Q8TCG1
• Cytogenetics: 3q13.13
• Protein Families: Druggable Genome
• MW: 102 kDa
• Gene Summary: Oncoprotein that inhibits PP2A and stabilizes MYC in human malignancies. Promotes anchorage-independent cell growth and tumor formation.[UniProtKB/Swiss-Prot Function]