NPIP (NPIPA1) (NM_006985) Human Tagged ORF Clone

CAT#: RC219505

NPIP (Myc-DDK-tagged)-Human nuclear pore complex interacting protein (NPIP). Note: ORF is codon optimized

Product Images

Circular map for RC219505

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: NPIP
• Synonyms: morpheus; NPIP; NPIPA
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC219505 ORF sequence, codon optimized.
  Due to the complexity of NM_006985, the ORF clone is codon optimized for mammalian Expression.
  The nucleotide sequence differs from the reference sequence, yet the amino acid sequence remains identical.
  
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTTTGTTGCCTGGGCTATGAATGGCTGAGCGGCGGGTGCAAGACATGGCATTCAGCATGGGTGATTA
  ATACACTCGCCGACCACAGGCACCGCGGTACGGACTTTGGGGGGTCCCCATGGCTGCTTATCATTACCGT
  ATTCCTCCGGTCATACAAGTTCGCTATCAGTCTCTGTACATCTTACCTTTGCGTATCATTCTTGAAGACC
  ATTTTCCCATCTCAGAATGGCCACGACGGGAGCACTGATGTCCAGCAAAGAGCTAGAAGGAGTAACCGGC
  GCCGGCAGGAGGGCATCAAGATCGTGCTGGAGGATATTTTTACACTGTGGAGGCAGGTGGAAACCAAGGT
  AAGGGCCAAGATACGCAAGATGAAAGTCACAACTAAGGTCAATCGGCACGATAAAATCAATGGAAAGAGA
  AAGACCGCAAAGGAACATCTGCGCAAGCTCTCCATGAAGGAGAGAGAGCACGGGGAGAAAGAACGGCAAG
  TCAGCGAAGCAGAGGAGAACGGAAAATTGGACATGAAAGAGATTCACACCTATATGGAGATGTTTCAGCG
  AGCGCAGGCTCTGCGGAGAAGAGCGGAAGATTATTACAGATGTAAAATTACCCCTTCAGCGAGAAAGCCC
  CTGTGTAACAGAGTACGAATGGCAGCTGTGGAACATAGACACTCAAGCGGCTTGCCCTATTGGCCCTATC
  TTACGGCCGAGACCTTGAAGAATCGAATGGGCCACCAGCCACCTCCCCCCACCCAGCAACACAGCATTAT
  TGACAACTCCCTGAGTCTGAAAACTCCCTCAGAGTGTCTGCTGACCCCTCTGCCGCCTTCTGCCCTCCCT
  TCCGCCGATGACAACCTCAAGACCCCTGCCGAATGCCTGTTGTATCCCTTGCCTCCATCCGCCGACGATA
  ACCTGAAGACCCCTCCTGAGTGTCTCCTCACGCCACTTCCCCCGTCTGCTCCGCCAAGCGTCGACGACAA
  TCTGAAAACCCCTCCAGAGTGCGTGTGCAGCTTGCCATTTCATCCACAGCGGATGATCATCAGCAGAAAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC219505 representing NM_006985
  Red=Cloning site Green=Tags(s)
  
  MFCCLGYEWLSGGCKTWHSAWVINTLADHRHRGTDFGGSPWLLIITVFLRSYKFAISLCTSYLCVSFLKT
  IFPSQNGHDGSTDVQQRARRSNRRRQEGIKIVLEDIFTLWRQVETKVRAKIRKMKVTTKVNRHDKINGKR
  KTAKEHLRKLSMKEREHGEKERQVSEAEENGKLDMKEIHTYMEMFQRAQALRRRAEDYYRCKITPSARKP
  LCNRVRMAAVEHRHSSGLPYWPYLTAETLKNRMGHQPPPPTQQHSIIDNSLSLKTPSECLLTPLPPSALP
  SADDNLKTPAECLLYPLPPSADDNLKTPPECLLTPLPPSAPPSVDDNLKTPPECVCSLPFHPQRMIISRN
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_006985
• ORF Size: 1050 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006985.1, NM_006985.2, NM_006985.3, NP_008916.1, NP_008916.2
• RefSeq Size: 1088 bp
• RefSeq ORF: 1053 bp
• Locus ID: 9284
• UniProt ID: Q9UND3
• Cytogenetics: 16p13.11
• MW: 40.1 kDa