GSDMA (NM_178171) Human Tagged ORF Clone

CAT#: RC219050

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GSDMA (Myc-DDK-tagged)-Human gasdermin A (GSDMA)



  "NM_178171" in other vectors (6)

Reconstitution Protocol

Special Offer: 20% off the control vector (empty cloning vector without insert). Use code "Shuttle20".

USD 477.00


Availability*
In Stock

Size
    • 10 ug


Product images

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol GSDMA
Synonyms FKSG9; GSDM; GSDM1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC219050 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGACCATGTTTGAAAATGTCACCCGGGCCCTGGCCAGACAGCTAAACCCTCGAGGGGACCTGACACCAC
TTGACAGCCTCATCGACTTCAAGCGCTTCCATCCCTTCTGCCTGGTGCTGAGGAAGAGGAAGAGCACGCT
CTTCTGGGGGGCCCGGTACGTCCGCACCGACTACACGCTGCTGGATGTGCTTGAGCCCGGCAGCTCACCT
TCAGACCCAACAGACACTGGGAATTTTGGCTTTAAGAATATGCTGGACACCCGAGTGGAGGGAGATGTGG
ATGTACCAAAGACGGTGAAGGTGAAGGGAACGGCAGGGCTCTCGCAGAACAGCACTCTGGAGGTCCAGAC
ACTCAGTGTGGCTCCCAAGGCCCTGGAGACCTTGCAGGAGAGGAAGCTGGCAGCAGACCACCCATTCCTG
AAGGAGATGCAAGATCAAGGGGAGAACCTGTATGTGGTGATGGAGGTGGTGGAGACGGTGCAGGAGGTCA
CACTGGAGCGAGCCGGCAAGGCAGAGGCCTGCTTCTCCCTCCCCTTCTTCGCCCCATTGGGGCTACAGGG
ATCCATAAATCACAAGGAGGCTGTAACCATCCCCAAGGGCTGCGTCCTGGCCTTTCGAGTGAGACAGCTG
ATGGTCAAAGGCAAAGATGAGTGGGATATTCCACATATCTGCAATGATAACATGCAAACCTTCCCTCCTG
GAGAAAAGTCAGGAGAGGAGAAGGTCATCCTTATCCAGGCATCTGATGTTGGGGACGTACACGAAGGCTT
CAGGACACTAAAAGAAGAAGTTCAGAGAGAGACCCAACAAGTGGAGAAGCTGAGCCGAGTAGGGCAAAGC
TCCCTGCTCAGCTCCCTCAGCAAACTTCTAGGGAAGAAAAAGGAGCTACAAGACCTTGAGCTCGCACTTG
AAGGGGCTCTAGACAAGGGACATGAAGTGAACCTGGAGGCACTCCCAAAAGATGTCCTGCTATCAAAGGA
GGCCGTGGGCGCCATCCTCTATTTCGTTGGAGCCCTAACAGAGCTAAGTGAAGCCCAACAGAAGCTGCTG
GTGAAATCCATGGAGAAAAAGATCCTACCCGTGCAGCTAAAGCTGGTGGAGAGCACGATGGAACAGAACT
TCCTGCTGGATAAAGAGGGTGTTTTCCCCCTGCAACCTGAGCTGCTCTCCTCCCTTGGGGACGAGGAGCT
GACCCTCACGGAGGCTCTAGTCGGGCTGAGTGGCCTGGAAGTGCAGAGATCGGGCCCCCAATATATGTGG
GACCCAGACACCCTCCCTCGCCTCTGTGCTCTTTATGCAGGCCTCTCTCTCCTTCAGCAGCTTACCAAGG
CCTCC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC219050 protein sequence
Red=Cloning site Green=Tags(s)

MTMFENVTRALARQLNPRGDLTPLDSLIDFKRFHPFCLVLRKRKSTLFWGARYVRTDYTLLDVLEPGSSP
SDPTDTGNFGFKNMLDTRVEGDVDVPKTVKVKGTAGLSQNSTLEVQTLSVAPKALETLQERKLAADHPFL
KEMQDQGENLYVVMEVVETVQEVTLERAGKAEACFSLPFFAPLGLQGSINHKEAVTIPKGCVLAFRVRQL
MVKGKDEWDIPHICNDNMQTFPPGEKSGEEKVILIQASDVGDVHEGFRTLKEEVQRETQQVEKLSRVGQS
SLLSSLSKLLGKKKELQDLELALEGALDKGHEVNLEALPKDVLLSKEAVGAILYFVGALTELSEAQQKLL
VKSMEKKILPVQLKLVESTMEQNFLLDKEGVFPLQPELLSSLGDEELTLTEALVGLSGLEVQRSGPQYMW
DPDTLPRLCALYAGLSLLQQLTKAS

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_178171
ORF Size 1335 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_178171.5
RefSeq Size 2164 bp
RefSeq ORF 1338 bp
Locus ID 284110
UniProt ID Q96QA5
Cytogenetics 17q21.1
MW 49.4 kDa
Gene Summary May promote pyroptosis (Probable). Upon cleavage in vitro of genetically engineered GSDMA, the released N-terminal moiety binds to some types of lipids, such as possibly phosphatidylinositol (4,5)-bisphosphate. Homooligomerizes within the membrane and forms pores of 10 -15 nanometers (nm) of inner diameter, triggering cell death. Also binds to bacterial and mitochondrial lipids, including cardiolipin, and exhibits bactericidal activity (PubMed:27281216). The physiological relevance of these observations is unknown (Probable).[UniProtKB/Swiss-Prot Function]

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