CSB (ERCC6) (NM_000124) Human Tagged ORF Clone

CAT#: RC219020

ERCC6 (Myc-DDK-tagged)-Human excision repair cross-complementing rodent repair deficiency, complementation group 6 (ERCC6)

Product Images

Circular map for RC219020

• 

  Western blot validation of overexpression lysate (Cat# LY424915) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC219020 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified ERCC6 protein (Cat# TP319020). The protein was produced from HEK293T cells transfected with ERCC6 cDNA clone (Cat# RC219020) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CSB
• Synonyms: ARMD5; CKN2; COFS; COFS1; CSB; CSB-PGBD3; POF11; RAD26; UVSS1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC219020 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCAAATGAGGGAATCCCCCACTCAAGTCAAACTCAGGAGCAAGACTGTTTACAGAGTCAACCTGTCA
  GTAATAATGAAGAAATGGCAATCAAGCAAGAAAGTGGTGGTGATGGGGAGGTGGAGGAGTACCTGTCCTT
  TCGTTCTGTGGGTGACGGGCTGTCCACCTCTGCTGTGGGGTGCGCATCAGCAGCTCCGAGGAGAGGGCCA
  GCCCTGCTGCACATCGACCGACATCAGATCCAGGCAGTAGAGCCTAGCGCCCAGGCCCTTGAGCTGCAGG
  GTTTGGGTGTGGACGTCTATGACCAGGACGTGCTGGAACAGGGAGTGCTTCAGCAGGTGGACAATGCCAT
  CCATGAGGCCAGCCGTGCCTCCCAGCTCGTTGACGTGGAGAAGGAGTATCGGTCGGTCCTGGATGACCTC
  ACGTCATGTACGACATCCCTAAGGCAAATCAATAAAATTATTGAACAGCTTAGCCCTCAAGCTGCCACCA
  GCAGAGACATCAACAGGAAACTAGATTCTGTAAAACGACAGAAGTATAATAAGGAACAACAGCTAAAAAA
  GATCACTGCAAAACAAAAGCATCTCCAGGCCATCCTTGGAGGAGCAGAGGTGAAAATTGAACTAGATCAC
  GCCAGTCTGGAGGAGGATGCAGAGCCGGGGCCATCCAGTCTTGGCAGCATGCTCATGCCTGTCCAGGAGA
  CTGCCTGGGAAGAGCTCATCCGCACTGGCCAGATGACACCTTTTGGTACCCAGATCCCTCAGAAACAGGA
  GAAAAAGCCCAGAAAAATCATGCTTAATGAAGCATCAGGCTTCGAAAAGTATTTGGCAGATCAAGCAAAA
  CTGTCTTTTGAAAGGAAGAAGCAAGGTTGTAATAAAAGAGCAGCTAGAAAAGCTCCAGCCCCAGTCACGC
  CTCCAGCCCCAGTGCAAAATAAAAACAAACCAAACAAGAAAGCCAGAGTTCTGTCCAAAAAAGAGGAGCG
  TTTGAAAAAGCACATCAAGAAACTCCAGAAGAGGGCTTTGCAGTTCCAGGGGAAAGTGGGATTGCCAAAG
  GCAAGGAGACCTTGGGAGTCAGACATGAGGCCAGAGGCAGAGGGAGACTCTGAGGGTGAAGAGTCTGAGT
  ATTTCCCCACAGAGGAGGAGGAAGAGGAGGAAGATGACGAGGTGGAGGGGGCAGAGGCGGACCTGTCTGG
  AGATGGTACTGACTATGAGCTGAAGCCTCTGCCCAAGGGCGGGAAACGGCAGAAGAAAGTGCCAGTGCAG
  GAGATTGATGATGACTTTTTCCCAAGTTCTGGGGAAGAAGCTGAAGCTGCTTCTGTAGGAGAAGGAGGAG
  GAGGAGGTCGGAAAGTGGGAAGATACCGAGATGATGGAGATGAAGATTATTATAAGCAGCGGTTAAGGAG
  ATGGAATAAACTGAGACTGCAGGACAAAGAGAAACGTCTGAAGCTGGAGGACGATTCTGAGGAAAGTGAT
  GCTGAATTTGACGAAGGTTTTAAAGTGCCAGGTTTTCTGTTCAAAAAGCTTTTTAAGTACCAGCAGACAG
  GTGTTAGGTGGCTGTGGGAATTGCACTGCCAGCAGGCAGGAGGAATTCTGGGAGATGAAATGGGATTGGG
  CAAGACCATCCAGATAATTGCCTTCTTGGCAGGTCTGAGCTACAGCAAGATCAGGACTCGTGGTTCAAAT
  TACAGGTTTGAGGGGTTGGGTCCAACTGTAATTGTCTGTCCAACAACAGTGATGCATCAGTGGGTGAAGG
  AATTTCACACGTGGTGGCCTCCGTTCAGAGTGGCAATTCTACATGAAACCGGTTCCTATACCCACAAAAA
  GGAGAAACTAATTCGAGATGTTGCTCATTGTCATGGAATTTTGATCACATCTTACTCCTACATTCGATTG
  ATGCAGGATGACATTAGCAGGTATGACTGGCACTATGTGATCTTGGACGAAGGACACAAAATTCGAAATC
  CAAATGCTGCTGTCACCCTTGCTTGCAAACAGTTTTGCACCCCTCATCGGATCATTCTGTCTGGCTCACC
  GATGCAAAATAACCTCCGAGAGCTGTGGTCGCTCTTTGACTTCATCTTCCCGGGAAAGTTAGGCACGTTG
  CCTGTGTTTATGGAGCAGTTCTCCGTCCCCATCACCATGGGGGGATATTCAAATGCTTCCCCAGTACAGG
  TCAAAACTGCTTACAAGTGTGCATGTGTCTTACGAGATACCATAAATCCATACCTACTGCGGAGAATGAA
  GTCAGATGTCAAGATGAGCCTTTCTTTGCCAGATAAAAATGAACAGGTCTTATTTTGCCGTCTTACAGAT
  GAGCAGCATAAAGTCTACCAAAATTTCGTTGATTCCAAAGAAGTTTACAGGATTCTCAATGGAGAGATGC
  AGATTTTCTCCGGACTTATAGCCCTAAGAAAAATTTGCAACCACCCTGATCTCTTTTCTGGAGGTCCCAA
  GAATCTCAAAGGTCTTCCTGATGATGAACTAGAAGAAGATCAGTTTGGGTACTGGAAACGTTCTGGGAAA
  ATGATTGTTGTTGAGTCTTTGTTGAAAATATGGCACAAGCAGGGTCAGCGAGTATTGCTGTTTTCTCAGT
  CAAGGCAGATGCTGGACATACTTGAAGTATTCCTTAGAGCCCAAAAGTATACCTATCTCAAGATGGATGG
  TACCACTACAATAGCTTCAAGACAGCCACTGATTACGAGATACAATGAGGACACATCCATATTTGTGTTT
  CTTCTGACCACGCGGGTGGGTGGCTTAGGTGTCAACCTGACGGGGGCAAACAGAGTTGTCATCTATGACC
  CAGACTGGAACCCAAGCACGGACACGCAGGCCCGGGAGCGAGCATGGAGAATAGGCCAGAAGAAGCAAGT
  GACTGTGTACAGGCTCCTGACTGCGGGCACCATTGAAGAAAAGATCTACCACCGACAAATCTTCAAGCAG
  TTTTTGACAAATAGAGTGCTAAAAGACCCAAAACAAAGGCGGTTTTTCAAATCCAATGATCTCTATGAGC
  TATTTACTCTGACTAGTCCTGATGCATCCCAGAGCACTGAAACAAGTGCAATTTTTGCAGGAACTGGATC
  AGATGTTCAGACACCCAAATGCCATCTAAAAAGAAGGATTCCACCAGCCTTTGGAGCAGACCATGATGTT
  CCAAAACGCAAGAAGTTCCCTGCTTCTAACATATCTGTAAATGATGCCACATCATCTGAAGAGAAATCTG
  AGGCTAAAGGAGCTGAAGTAAATGCAGTAACTTCTAATCGAAGTGATCCTTTGAAAGATGACCCTCACAT
  GAGTAGTAATGTAACTAGCAATGATAGGCTTGGAGAAGAGACAAATGCAGTATCTGGACCAGAAGAGTTG
  TCAGTGATTAGTGGAAATGGGGAATGTTCAAATTCTTCAGGAACAGGCAAAACTTCTATGCCATCTGGTG
  ATGAAAGCATTGATGAAAAGTTAGGTCTTTCTTACAAAAGAGAAAGACCCAGCCAGGCTCAAACAGAAGC
  TTTTTGGGAGAATAAACAAATGGAAAATAATTTTTATAAGCACAAGTCAAAAACAAAACATCATAGTGTG
  GCAGAAGAAGAGACCCTGGAGAAACATCTGAGACCAAAGCAAAAGCCTAAGAACTCTAAGCATTGCAGAG
  ACGCCAAGTTTGAAGGAACTCGAATTCCACACCTGGTGAAGAAAAGGCGTTACCAGAAGCAAGACAGTGA
  AAACAAGAGTGAGGCCAAGGAACAGAGCAATGACGATTATGTTTTGGAAAAGCTTTTCAAAAAATCAGTT
  GGCGTGCACAGTGTCATGAAGCACGATGCCATCATGGATGGAGCCAGCCCAGATTATGTACTGGTGGAGG
  CAGAAGCCAACCGAGTGGCCCAGGATGCCCCGAAAGCACTGAGGCTCTCTCGTCAGCGGTGTCTGGGAGC
  AGTGTCTGGTGTTCCCACCTGGACTGGCCACAGGGGGATTTCTGGTGCACCAGCAGGAAAAAAGAGTAGA
  TTTGGTAAGAAAAGGAATTCTAACTTCTCTGTGCAGCATCCTTCATCAACATCTCCAACAGAGAAGTGCC
  AGGATGGCATCATGAAAAAGGAGGGAAAAGATAATGTCCCTGAGCATTTTAGTGGAAGAGCAGAAGATGC
  AGACTCTTCATCCGGGCCCCTCGCTTCCTCCTCACTCTTGGCTAAAATGAGAGCTAGAAACCACCTGATT
  CTGCCAGAGCGTTTAGAAAGTGAAAGCGGGCACCTGCAGGAAGCTTCTGCCCTGCTGCCCACCACAGAAC
  ACGATGACCTTCTGGTGGAGATGAGAAACTTCATCGCTTTCCAGGCCCACACTGATGGCCAGGCCAGCAC
  CAGGGAGATACTGCAGGAGTTTGAATCCAAGTTATCTGCATCACAGTCTTGTGTCTTCCGAGAACTATTG
  AGAAATCTGTGCACTTTCCATAGAACTTCTGGTGGTGAAGGAATTTGGAAACTCAAGCCAGAATACTGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC219020 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MPNEGIPHSSQTQEQDCLQSQPVSNNEEMAIKQESGGDGEVEEYLSFRSVGDGLSTSAVGCASAAPRRGP
  ALLHIDRHQIQAVEPSAQALELQGLGVDVYDQDVLEQGVLQQVDNAIHEASRASQLVDVEKEYRSVLDDL
  TSCTTSLRQINKIIEQLSPQAATSRDINRKLDSVKRQKYNKEQQLKKITAKQKHLQAILGGAEVKIELDH
  ASLEEDAEPGPSSLGSMLMPVQETAWEELIRTGQMTPFGTQIPQKQEKKPRKIMLNEASGFEKYLADQAK
  LSFERKKQGCNKRAARKAPAPVTPPAPVQNKNKPNKKARVLSKKEERLKKHIKKLQKRALQFQGKVGLPK
  ARRPWESDMRPEAEGDSEGEESEYFPTEEEEEEEDDEVEGAEADLSGDGTDYELKPLPKGGKRQKKVPVQ
  EIDDDFFPSSGEEAEAASVGEGGGGGRKVGRYRDDGDEDYYKQRLRRWNKLRLQDKEKRLKLEDDSEESD
  AEFDEGFKVPGFLFKKLFKYQQTGVRWLWELHCQQAGGILGDEMGLGKTIQIIAFLAGLSYSKIRTRGSN
  YRFEGLGPTVIVCPTTVMHQWVKEFHTWWPPFRVAILHETGSYTHKKEKLIRDVAHCHGILITSYSYIRL
  MQDDISRYDWHYVILDEGHKIRNPNAAVTLACKQFCTPHRIILSGSPMQNNLRELWSLFDFIFPGKLGTL
  PVFMEQFSVPITMGGYSNASPVQVKTAYKCACVLRDTINPYLLRRMKSDVKMSLSLPDKNEQVLFCRLTD
  EQHKVYQNFVDSKEVYRILNGEMQIFSGLIALRKICNHPDLFSGGPKNLKGLPDDELEEDQFGYWKRSGK
  MIVVESLLKIWHKQGQRVLLFSQSRQMLDILEVFLRAQKYTYLKMDGTTTIASRQPLITRYNEDTSIFVF
  LLTTRVGGLGVNLTGANRVVIYDPDWNPSTDTQARERAWRIGQKKQVTVYRLLTAGTIEEKIYHRQIFKQ
  FLTNRVLKDPKQRRFFKSNDLYELFTLTSPDASQSTETSAIFAGTGSDVQTPKCHLKRRIPPAFGADHDV
  PKRKKFPASNISVNDATSSEEKSEAKGAEVNAVTSNRSDPLKDDPHMSSNVTSNDRLGEETNAVSGPEEL
  SVISGNGECSNSSGTGKTSMPSGDESIDEKLGLSYKRERPSQAQTEAFWENKQMENNFYKHKSKTKHHSV
  AEEETLEKHLRPKQKPKNSKHCRDAKFEGTRIPHLVKKRRYQKQDSENKSEAKEQSNDDYVLEKLFKKSV
  GVHSVMKHDAIMDGASPDYVLVEAEANRVAQDAPKALRLSRQRCLGAVSGVPTWTGHRGISGAPAGKKSR
  FGKKRNSNFSVQHPSSTSPTEKCQDGIMKKEGKDNVPEHFSGRAEDADSSSGPLASSSLLAKMRARNHLI
  LPERLESESGHLQEASALLPTTEHDDLLVEMRNFIAFQAHTDGQASTREILQEFESKLSASQSCVFRELL
  RNLCTFHRTSGGEGIWKLKPEYC
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_000124
• ORF Size: 4479 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000124.4
• RefSeq Size: 8993 bp
• RefSeq ORF: 4482 bp
• Locus ID: 2074
• UniProt ID: Q03468
• Cytogenetics: 10q11.23
• Domains: SNF2_N, DEAD, helicase_C
• Protein Families: Druggable Genome
• Protein Pathways: Nucleotide excision repair
• MW: 168.3 kDa
• Gene Summary: This gene encodes a DNA-binding protein that is important in transcription-coupled excision repair. The encoded protein has ATP-stimulated ATPase activity, interacts with several transcription and excision repair proteins, and may promote complex formation at DNA repair sites. Mutations in this gene are associated with Cockayne syndrome type B and cerebrooculofacioskeletal syndrome 1. Alternative splicing occurs between a splice site from exon 5 of this gene to the 3' splice site upstream of the open reading frame (ORF) of the adjacent gene, piggyback-derived-3 (GeneID:267004), which activates the alternative polyadenylation site downstream of the piggyback-derived-3 ORF. The resulting transcripts encode a fusion protein that shares sequence with the product of each individual gene. [provided by RefSeq, Mar 2016]