CGK2 (PRKG2) (NM_006259) Human Tagged ORF Clone

CAT#: RC215879

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PRKG2 (Myc-DDK-tagged)-Human protein kinase, cGMP-dependent, type II (PRKG2)



  "NM_006259" in other vectors (7)

Reconstitution Protocol

USD 698.00


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Size
    • 10 ug


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Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol PRKG2
Synonyms cGK2; cGKII; PKG2; PRKGR2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC215879 representing NM_006259
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGAAATGGTTCAGTGAAACCTAAACATTCTAAGCACCCAGATGGACACTCTGGGAACCTCACCACTG
ATGCTCTGCGGAACAAGGTGACAGAGCTGGAGAGAGAGTTGAGGAGGAAGGATGCTGAGATCCAGGAGCG
GGAGTACCATTTGAAGGAGCTGCGGGAGCAGCTGTCGAAGCAGACTGTGGCCATTGCTGAACTCACAGAG
GAGCTCCAGAACAAGTGCATCCAGCTGAACAAGCTGCAGGATGTGGTGCATATGCAGGGAGGAAGCCCGC
TTCAGGCCTCTCCAGATAAAGTGCCTCTTGAGGTCCACCGGAAGACCTCTGGATTGGTCTCTCTCCATAG
CAGGAGGGGAGCAAAGGCTGGCGTGTCTGCTGAGCCAACAACCCGGACCTATGACCTGAACAAACCCCCT
GAATTTTCCTTTGAGAAAGCAAGAGTCAGAAAAGACTCCAGTGAGAAGAAGCTCATTACAGATGCCCTTA
ATAAAAATCAGTTTCTGAAAAGACTGGATCCTCAGCAGATCAAAGACATGGTGGAATGCATGTATGGGAG
AAACTATCAGCAAGGGAGTTACATTATTAAGCAAGGAGAACCAGGAAACCATATCTTTGTGCTGGCAGAG
GGTCGACTAGAGGTGTTCCAAGGGGAGAAATTGCTGTCCTCCATCCCTATGTGGACCACATTTGGGGAGC
TTGCCATTTTATACAATTGTACAAGGACTGCCTCTGTGAAAGCTATTACCAATGTTAAAACATGGGCACT
AGATCGAGAGGTATTCCAGAATATAATGAGGAGGACAGCCCAAGCTAGAGATGAACAATACAGAAACTTC
CTCAGAAGTGTATCCTTGCTGAAGAATTTACCTGAAGATAAATTAACCAAGATCATTGACTGCTTGGAAG
TGGAATACTATGACAAAGGAGATTACATCATTAGAGAGGGCGAGGAAGGAAGTACCTTTTTCATTTTGGC
AAAAGGAAAGGTAAAAGTAACACAGAGCACAGAAGGCCATGATCAACCACAGCTGATAAAAACACTGCAG
AAAGGAGAATACTTTGGAGAAAAAGCTCTTATCAGTGATGATGTCAGGTCAGCTAACATTATTGCTGAAG
AAAATGATGTTGCATGCCTGGTTATAGATCGAGAAACATTCAACCAAACTGTCGGTACATTTGAAGAGCT
GCAAAAATACCTTGAAGGATATGTGGCAAACCTGAACCGTGATGATGAAAAAAGACATGCGAAGCGGTCC
ATGTCTAACTGGAAGCTGTCCAAAGCACTCTCTCTGGAAATGATTCAGCTGAAGGAGAAGGTGGCCAGAT
TTTCCTCATCATCCCCATTCCAGAACCTTGAGATTATTGCAACACTGGGCGTTGGTGGGTTCGGAAGAGT
TGAGCTTGTTAAAGTAAAAAATGAGAATGTTGCTTTTGCTATGAAGTGTATAAGGAAGAAGCACATAGTT
GACACCAAGCAGCAGGAGCATGTCTACTCAGAGAAGAGGATCCTAGAGGAGCTGTGCTCTCCATTCATTG
TGAAATTATATCGTACTTTCAAGGACAATAAGTATGTATACATGCTTCTGGAGGCCTGCTTAGGTGGGGA
GCTCTGGAGTATATTAAGGGACAGAGGCAGCTTTGATGAACCCACCTCCAAATTCTGCGTTGCTTGTGTG
ACAGAAGCATTTGATTACCTGCATCGACTAGGTATTATCTACAGAGACTTGAAACCAGAAAACTTAATTC
TAGATGCTGAGGGTTACCTTAAATTGGTTGACTTTGGATTTGCGAAGAAAATAGGGTCTGGACAGAAAAC
ATGGACATTCTGTGGGACTCCAGAATATGTAGCTCCTGAAGTCATTCTCAACAAGGGACATGACTTCAGT
GTGGATTTCTGGTCACTGGGAATTCTAGTGTATGAGCTCCTAACGGGCAACCCACCCTTTTCTGGGGTTG
ACCAAATGATGACCTACAATTTGATTCTCAAAGGAATTGAAAAAATGGATTTTCCCAGGAAGATAACACG
ACGACCTGAGGATTTGATTCGGAGGCTTTGCAGGCAAAATCCAACAGAAAGGCTGGGAAATCTGAAGAAT
GGAATAAATGACATTAAGAAACACAGGTGGTTAAATGGTTTTAATTGGGAGGGACTGAAAGCACGGAGCC
TTCCATCACCTTTGCAAAGAGAGCTCAAGGGACCCATAGATCACAGCTACTTTGACAAATATCCTCCTGA
AAAGGGAATGCCTCCAGATGAGCTATCAGGCTGGGATAAAGACTTC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC215879 representing NM_006259
Red=Cloning site Green=Tags(s)

MGNGSVKPKHSKHPDGHSGNLTTDALRNKVTELERELRRKDAEIQEREYHLKELREQLSKQTVAIAELTE
ELQNKCIQLNKLQDVVHMQGGSPLQASPDKVPLEVHRKTSGLVSLHSRRGAKAGVSAEPTTRTYDLNKPP
EFSFEKARVRKDSSEKKLITDALNKNQFLKRLDPQQIKDMVECMYGRNYQQGSYIIKQGEPGNHIFVLAE
GRLEVFQGEKLLSSIPMWTTFGELAILYNCTRTASVKAITNVKTWALDREVFQNIMRRTAQARDEQYRNF
LRSVSLLKNLPEDKLTKIIDCLEVEYYDKGDYIIREGEEGSTFFILAKGKVKVTQSTEGHDQPQLIKTLQ
KGEYFGEKALISDDVRSANIIAEENDVACLVIDRETFNQTVGTFEELQKYLEGYVANLNRDDEKRHAKRS
MSNWKLSKALSLEMIQLKEKVARFSSSSPFQNLEIIATLGVGGFGRVELVKVKNENVAFAMKCIRKKHIV
DTKQQEHVYSEKRILEELCSPFIVKLYRTFKDNKYVYMLLEACLGGELWSILRDRGSFDEPTSKFCVACV
TEAFDYLHRLGIIYRDLKPENLILDAEGYLKLVDFGFAKKIGSGQKTWTFCGTPEYVAPEVILNKGHDFS
VDFWSLGILVYELLTGNPPFSGVDQMMTYNLILKGIEKMDFPRKITRRPEDLIRRLCRQNPTERLGNLKN
GINDIKKHRWLNGFNWEGLKARSLPSPLQRELKGPIDHSYFDKYPPEKGMPPDELSGWDKDF

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_006259
ORF Size 2286 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_006259.2
RefSeq Size 3328 bp
RefSeq ORF 2289 bp
Locus ID 5593
UniProt ID Q13237
Cytogenetics 4q21.21
Domains cNMP, pkinase, S_TK_X, TyrKc, S_TKc
Protein Families Druggable Genome, Protein Kinase
Protein Pathways Gap junction, Long-term depression, Olfactory transduction
MW 87.3 kDa
Gene Summary This gene encodes a protein that belongs to the serine/threonine protein kinase family of proteins. The encoded protein binds to and inhibits the activation of several receptor tyrosine kinases. The membrane-bound protein is a regulator of intestinal secretion, bone growth and renin secretion. Alternate splicing results in multiple transcript variants encoding distinct isoforms whose regulatory N-termini differ in length but whose C-terminal catalytic domains are identical. [provided by RefSeq, May 2018]

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