APOBEC1 (NM_001644) Human Tagged ORF Clone
APOBEC1 (Myc-DDK-tagged)-Human apolipoprotein B mRNA editing enzyme, catalytic polypeptide 1 (APOBEC1)
|Product Name||APOBEC1 (NM_001644) Human Tagged ORF Clone|
|Type||Human Tagged ORF Clone|
|Synonyms||APOBEC-1; BEDP; CDAR1; HEPR|
|E. coli Selection||Kanamycin (25 ug/mL)|
>RC215744 representing NM_001644
Red=Cloning site Blue=ORF Green=Tags(s)
>RC215744 representing NM_001644
Red=Cloning site Green=Tags(s)
Sequencher program is needed, download here.
|Restriction Sites||SgfI-MluI Cloning Scheme for this gene Plasmid Map|
|ORF Size||708 bp|
|OTI Disclaimer||Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at firstname.lastname@example.org or by calling 301.340.3188 option 3 for pricing and delivery.
The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
|OTI Annotation||This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.|
|Product Components||The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.|
|Reconstitution||1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
|RefSeq||NM_001644.1, NM_001644.2, NM_001644.3, NM_001644.4, NP_001635.2|
|RefSeq Size||911 bp|
|RefSeq ORF||711 bp|
|Gene Summary||This gene encodes a member of the cytidine deaminase enzyme family. The encoded protein forms a multiple-protein editing holoenzyme with APOBEC1 complementation factor (ACF) and APOBEC1 stimulating protein (ASP). This holoenzyme is involved in the editing of C-to-U nucleotide bases in apolipoprotein B and neurofibromatosis-1 mRNAs. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2015]|
|The use of this cDNA Clones has been cited in the following citations:|
A Novel RNA Editing Sensor Tool and a Specific Agonist Determine Neuronal Protein Expression of RNA-Edited Glycine Receptors and Identify a Genomic APOBEC1 Dimorphism as a New Genetic Risk Factor of Epilepsy
,Kankowski, S;Förstera, B;Winkelmann, A;Knauff, P;Wanker, EE;You, XA;Semtner, M;Hetsch, F;Meier, JC;,
Front Mol Neurosci 2017
,PubMed ID 29375302
|cDNA Clone Resources|
|SC303051||APOBEC1 (untagged)-Human apolipoprotein B mRNA editing enzyme, catalytic polypeptide 1 (APOBEC1)||
|RG215744||APOBEC1 (GFP-tagged) - Human apolipoprotein B mRNA editing enzyme, catalytic polypeptide 1 (APOBEC1)||
|RC215744L1||Lenti ORF clone of Human apolipoprotein B mRNA editing enzyme, catalytic polypeptide 1 (APOBEC1), Myc-DDK-tagged||
|RC215744L2||Lenti ORF clone of Human apolipoprotein B mRNA editing enzyme, catalytic polypeptide 1 (APOBEC1), mGFP tagged||
|RC215744L3||Lenti ORF clone of Human apolipoprotein B mRNA editing enzyme, catalytic polypeptide 1 (APOBEC1), Myc-DDK-tagged||
|RC215744L4||Lenti ORF clone of Human apolipoprotein B mRNA editing enzyme, catalytic polypeptide 1 (APOBEC1), mGFP tagged||