KCNC1 (NM_004976) Human Tagged ORF Clone

CAT#: RC215716

KCNC1 (Myc-DDK-tagged)-Human potassium voltage-gated channel, Shaw-related subfamily, member 1 (KCNC1), transcript variant B

Product Images

Western blot validation of overexpression lysate (Cat# LY417608) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC215716 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: KCNC1
• Synonyms: EPM7; KV3.1; KV4; NGK2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC215716 representing NM_004976
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCCAAGGGGACGAGAGCGAGCGCATCGTGATCAACGTGGGCGGCACGCGCCACCAGACGTACCGCT
  CGACCCTGCGCACGCTGCCCGGCACGCGGCTCGCCTGGCTGGCGGAGCCCGACGCCCACAGCCACTTCGA
  CTATGACCCGCGTGCTGACGAGTTCTTCTTCGACCGCCACCCCGGCGTCTTCGCGCACATCCTGAACTAC
  TACCGCACGGGCAAGCTGCACTGCCCAGCCGACGTGTGCGGGCCGCTCTACGAGGAGGAGCTGGCCTTCT
  GGGGCATCGACGAGACCGACGTGGAGCCCTGCTGCTGGATGACGTACCGCCAGCACCGCGACGCCGAGGA
  GGCTCTGGACAGCTTCGGCGGCGCTCCTCTGGACAACAGCGCCGACGACGCGGACGCCGACGGCCCTGGC
  GACTCGGGCGACGGCGAGGACGAGCTGGAGATGACCAAGCGCCTGGCGCTCAGTGACTCCCCGGATGGCC
  GGCCTGGCGGCTTTTGGCGCCGCTGGCAGCCGCGCATCTGGGCGCTCTTCGAGGACCCGTACTCGTCCCG
  CTACGCGCGGTATGTGGCCTTCGCTTCCCTCTTCTTCATCCTGGTCTCCATCACCACCTTCTGCCTGGAG
  ACCCACGAGCGCTTCAACCCCATCGTGAACAAGACGGAGATCGAGAACGTTCGCAATGGCACGCAAGTGC
  GCTACTACCGGGAGGCCGAGACGGAGGCCTTCCTTACCTACATCGAGGGCGTCTGTGTGGTCTGGTTCAC
  CTTCGAGTTCCTCATGCGTGTCATCTTCTGCCCCAACAAGGTAGAGTTCATCAAGAACTCGCTCAACATC
  ATTGACTTTGTGGCCATCCTGCCCTTCTACCTGGAGGTGGGGCTGAGCGGCCTGTCCTCCAAGGCAGCCA
  AGGACGTGCTGGGCTTCCTGCGCGTCGTCCGCTTCGTGCGCATCTTGCGCATCTTTAAGCTGACCCGCCA
  CTTTGTGGGCCTGCGGGTCCTGGGCCACACGCTCCGAGCCAGCACCAACGAGTTCCTGCTGCTCATCATC
  TTCCTGGCCTTGGGCGTGCTGATCTTCGCCACCATGATCTACTACGCCGAGAGGATAGGGGCACAGCCCA
  ATGACCCCAGCGCCAGTGAGCACACGCACTTTAAGAACATCCCCATCGGCTTCTGGTGGGCCGTGGTCAC
  CATGACGACCCTGGGCTATGGAGACATGTACCCGCAGACGTGGTCCGGCATGCTGGTGGGGGCTCTGTGT
  GCGCTGGCGGGCGTGCTCACCATCGCCATGCCCGTGCCCGTCATCGTGAACAATTTCGGGATGTATTACT
  CCTTAGCCATGGCTAAGCAGAAACTACCAAAGAAAAAAAAGAAGCATATTCCGCGGCCACCGCAGCTGGG
  ATCTCCCAATTATTGTAAATCTGTCGTAAACTCTCCACACCACAGTACTCAGAGTGACACATGTCCGCTG
  GCCCAGGAAGAAATTTTAGAAATTAACAGAGCAGGTAGGAAACCTCTTAGAGGCATGTCGATC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC215716 representing NM_004976
  Red=Cloning site Green=Tags(s)
  
  MGQGDESERIVINVGGTRHQTYRSTLRTLPGTRLAWLAEPDAHSHFDYDPRADEFFFDRHPGVFAHILNY
  YRTGKLHCPADVCGPLYEEELAFWGIDETDVEPCCWMTYRQHRDAEEALDSFGGAPLDNSADDADADGPG
  DSGDGEDELEMTKRLALSDSPDGRPGGFWRRWQPRIWALFEDPYSSRYARYVAFASLFFILVSITTFCLE
  THERFNPIVNKTEIENVRNGTQVRYYREAETEAFLTYIEGVCVVWFTFEFLMRVIFCPNKVEFIKNSLNI
  IDFVAILPFYLEVGLSGLSSKAAKDVLGFLRVVRFVRILRIFKLTRHFVGLRVLGHTLRASTNEFLLLII
  FLALGVLIFATMIYYAERIGAQPNDPSASEHTHFKNIPIGFWWAVVTMTTLGYGDMYPQTWSGMLVGALC
  ALAGVLTIAMPVPVIVNNFGMYYSLAMAKQKLPKKKKKHIPRPPQLGSPNYCKSVVNSPHHSTQSDTCPL
  AQEEILEINRAGRKPLRGMSI
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_004976
• ORF Size: 1533 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004976.4
• RefSeq Size: 1602 bp
• RefSeq ORF: 1536 bp
• Locus ID: 3746
• UniProt ID: P48547
• Cytogenetics: 11p15.1
• Protein Families: Druggable Genome, Ion Channels: Potassium, Transmembrane
• MW: 57.8 kDa
• Gene Summary: This gene encodes a member of a family of integral membrane proteins that mediate the voltage-dependent potassium ion permeability of excitable membranes. Alternative splicing is thought to result in two transcript variants encoding isoforms that differ at their C-termini. These isoforms have had conflicting names in the literature: the longer isoform has been called both "b" and "alpha", while the shorter isoform has been called both "a" and "beta" (PMIDs 1432046, 12091563). [provided by RefSeq, Oct 2014]