NR5A2 (NM_003822) Human Tagged ORF Clone

CAT#: RC213887

NR5A2 (Myc-DDK-tagged)-Human nuclear receptor subfamily 5, group A, member 2 (NR5A2), transcript variant 2

Product Images

Western blot validation of overexpression lysate (Cat# LY401255) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC213887 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: NR5A2
• Synonyms: B1F; B1F2; CPF; FTF; FTZ-F1; FTZ-F1beta; hB1F-2; LRH-1; LRH1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC213887 representing NM_003822
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTTCTAATTCAGATACTGGGGATTTACAAGAGTCTTTAAAGCACGGACTTACACCTATTGTGTCTC
  AATTTAAAATGGTGAATTACTCCTATGATGAAGATCTGGAAGAGCTTTGTCCCGTGTGTGGAGATAAAGT
  GTCTGGGTACCATTATGGGCTCCTCACCTGTGAAAGCTGCAAGGGATTTTTTAAGCGAACAGTCCAAAAT
  AATAAAAGGTACACATGTATAGAAAACCAGAACTGCCAAATTGACAAAACACAGAGAAAGCGTTGTCCTT
  ACTGTCGTTTTCAAAAATGTCTAAGTGTTGGAATGAAGCTAGAAGCTGTAAGGGCCGACCGAATGCGTGG
  AGGAAGGAATAAGTTTGGGCCAATGTACAAGAGAGACAGGGCCCTGAAGCAACAGAAAAAAGCCCTCATC
  CGAGCCAATGGACTTAAGCTAGAAGCCATGTCTCAGGTGATCCAAGCTATGCCCTCTGACCTGACCATTT
  CCTCTGCAATTCAAAACATCCACTCTGCCTCCAAAGGCCTACCTCTGAACCATGCTGCCTTGCCTCCTAC
  AGACTATGACAGAAGTCCCTTTGTAACATCCCCCATTAGCATGACAATGCCCCCTCACGGCAGCCTGCAA
  GGTTACCAAACACATGGCCACTTTCCTAGCCGGGCCATCAAGTCTGAGTACCCAGACCCCTATACCAGCT
  CACCCGAGTCCATAATGGGCTATTCATATATGGATAGTTACCAGACGAGCTCTCCAGCAAGCATCCCACA
  TCTGATACTGGAACTTTTGAAGTGTGAGCCAGATGAGCCTCAAGTCCAGGCTAAAATCATGGCCTATTTG
  CAGCAAGAGCAGGCTAACCGAAGCAAGCACGAAAAGCTGAGCACCTTTGGGCTTATGTGCAAAATGGCAG
  ATCAAACTCTCTTCTCCATTGTCGAGTGGGCCAGGAGTAGTATCTTCTTCAGAGAACTTAAGGTTGATGA
  CCAAATGAAGCTGCTTCAGAACTGCTGGAGTGAGCTCTTAATCCTCGACCACATTTACCGACAAGTGGTA
  CATGGAAAGGAAGGATCCATCTTCCTGGTTACTGGGCAACAAGTGGACTATTCCATAATAGCATCACAAG
  CCGGAGCCACCCTCAACAACCTCATGAGTCATGCACAGGAGTTAGTGGCAAAACTTCGTTCTCTCCAGTT
  TGATCAACGAGAGTTCGTATGTCTGAAATTCTTGGTGCTCTTTAGTTTAGATGTCAAAAACCTTGAAAAC
  TTCCAGCTGGTAGAAGGTGTCCAGGAACAAGTCAATGCCGCCCTGCTGGACTACACAATGTGTAACTACC
  CGCAGCAGACAGAGAAATTTGGACAGCTACTTCTTCGACTACCCGAAATCCGGGCCATCAGTATGCAGGC
  TGAAGAATACCTCTACTACAAGCACCTGAACGGGGATGTGCCCTATAATAACCTTCTCATTGAAATGTTG
  CATGCCAAAAGAGCA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC213887 representing NM_003822
  Red=Cloning site Green=Tags(s)
  
  MSSNSDTGDLQESLKHGLTPIVSQFKMVNYSYDEDLEELCPVCGDKVSGYHYGLLTCESCKGFFKRTVQN
  NKRYTCIENQNCQIDKTQRKRCPYCRFQKCLSVGMKLEAVRADRMRGGRNKFGPMYKRDRALKQQKKALI
  RANGLKLEAMSQVIQAMPSDLTISSAIQNIHSASKGLPLNHAALPPTDYDRSPFVTSPISMTMPPHGSLQ
  GYQTHGHFPSRAIKSEYPDPYTSSPESIMGYSYMDSYQTSSPASIPHLILELLKCEPDEPQVQAKIMAYL
  QQEQANRSKHEKLSTFGLMCKMADQTLFSIVEWARSSIFFRELKVDDQMKLLQNCWSELLILDHIYRQVV
  HGKEGSIFLVTGQQVDYSIIASQAGATLNNLMSHAQELVAKLRSLQFDQREFVCLKFLVLFSLDVKNLEN
  FQLVEGVQEQVNAALLDYTMCNYPQQTEKFGQLLLRLPEIRAISMQAEEYLYYKHLNGDVPYNNLLIEML
  HAKRA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_003822
• ORF Size: 1485 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003822.5
• RefSeq Size: 4916 bp
• RefSeq ORF: 1488 bp
• Locus ID: 2494
• UniProt ID: O00482
• Cytogenetics: 1q32.1
• Domains: HOLI, zf-C4
• Protein Families: Druggable Genome, Nuclear Hormone Receptor, Transcription Factors
• Protein Pathways: Maturity onset diabetes of the young
• MW: 56.3 kDa
• Gene Summary: The protein encoded by this gene is a DNA-binding zinc finger transcription factor and is a member of the fushi tarazu factor-1 subfamily of orphan nuclear receptors. The encoded protein is involved in the expression of genes for hepatitis B virus and cholesterol biosynthesis, and may be an important regulator of embryonic development. [provided by RefSeq, Jun 2016]