NR5A2 (NM_003822) Human Untagged Clone

SKU SC117736

NR5A2 (untagged)-Human nuclear receptor subfamily 5, group A, member 2 (NR5A2), transcript variant 2

Product Data

• Type: Human Untagged Clone
• Target Symbol: NR5A2
• Synonyms: B1F; B1F2; CPF; FTF; FTZ-F1; FTZ-F1beta; hB1F-2; LRH-1; LRH1
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_003822 edited; ATGTCTTCTAATTCAGATACTGGGGATTTACAAGAGTCTTTAAAGCACGGACTTACACCT; ATTGTGTCTCAATTTAAAATGGTGAATTACTCCTATGATGAAGATCTGGAAGAGCTTTGT; CCCGTGTGTGGAGATAAAGTGTCTGGGTACCATTATGGGCTCCTCACCTGTGAAAGCTGC; AAGGGATTTTTTAAGCGAACAGTCCAAAATAATAAAAGGTACACATGTATAGAAAACCAG; AACTGCCAAATTGACAAAACACAGAGAAAGCGTTGTCCTTACTGTCGTTTTCAAAAATGT; CTAAGTGTTGGAATGAAGCTAGAAGCTGTAAGGGCCGACCGAATGCGTGGAGGAAGGAAT; AAGTTTGGGCCAATGTACAAGAGAGACAGGGCCCTGAAGCAACAGAAAAAAGCCCTCATC; CGAGCCAATGGACTTAAGCTAGAAGCCATGTCTCAGGTGATCCAAGCTATGCCCTCTGAC; CTGACCATTTCCTCTGCAATTCAAAACATCCACTCTGCCTCCAAAGGCCTACCTCTGAAC; CATGCTGCCTTGCCTCCTACAGACTATGACAGAAGTCCCTTTGTAACATCCCCCATTAGC; ATGACAATGCCCCCTCACGGCAGCCTGCAAGGTTACCAAACACATGGCCACTTTCCTAGC; CGGGCCATCAAGTCTGAGTACCCAGACCCCTATACCAGCTCACCCGAGTCCATAATGGGC; TATTCATATATGGATAGTTACCAGACGAGCTCTCCAGCAAGCATCCCACATCTGATACTG; GAACTTTTGAAGTGTGAGCCAGATGAGCCTCAAGTCCAGGCTAAAATCATGGCCTATTTG; CAGCAAGAGCAGGCTAACCGAAGCAAGCACGAAAAGCTGAGCACCTTTGGGCTTATGTGC; AAAATGGCAGATCAAACTCTCTTCTCCATTGTCGAGTGGGCCAGGAGTAGTATCTTCTTC; AGAGAACTTAAGGTTGATGACCAAATGAAGCTGCTTCAGAACTGCTGGAGTGAGCTCTTA; ATCCTCGACCACATTTACCGACAAGTGGTACATGGAAAGGAAGGATCCATCTTCCTGGTT; ACTGGGCAACAAGTGGACTATTCCATAATAGCATCACAAGCCGGAGCCACCCTCAACAAC; CTCATGAGTCATGCACAGGAGTTAGTGGCAAAACTTCGTTCTCTCCAGTTTGATCAACGA; GAGTTCGTATGTCTGAAATTCTTGGTGCTCTTTAGTTTAGATGTCAAAAACCTTGAAAAC; TTCCAGCTGGTAGAAGGTGTCCAGGAACAAGTCAATGCCGCCCTGCTGGACTACACAATG; TGTAACTACCCGCAGCAGACAGAGAAATTTGGACAGCTACTTCTTCGACTACCCGAAATC; CGGGCCATCAGTATGCAGGCTGAAGAATACCTCTACTACAAGCACCTGAACGGGGATGTG; CCCTATAATAACCTTCTCATTGAAATGTTGCATGCCAAAAGAGCATAA;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_003822 unedited; GGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGGGNAAAACTTGCTTGTAAC; TTTATGAATTCTGGATTTTTTTTTTTCCTTTGCTTTTTCTTAACTTTCACTAAGGGTTAC; TGTAGTCTGATGTGTCCTTCCCAAGGCCACGAAATTTGACAAGCTGCACTTTTCTTTTGC; TCAATGATTTCTGCTTTAAGCCAAAGAACTGCCTATAATTTCACTAAGAATGTCTTCTAA; TTCAGATACTGGGGATTTACAAGAGTCTTTAAAGCACGGACTTACACCTATTGTGTCTCA; ATTTAAAATGGTGAATTACTCCTATGATGAAGATCTGGAAGAGCTTTGTCCCGTGTGTGG; AGATAAAGTGTCTGGGTACCATTATGGGCTCCTCACCTGTGAAAGCTGCAAGGGATTTTT; TAAGCGAACAGTCCAAAATAATAAAAGGTACACATGTATAGAAAACCAGAACTGCCAAAT; TGACAAAACACAGAGAAAGCGTTGTCCTTACTGTCGTTTTCAAAAATGTCTAAGTGTTGG; AATGAAGCTAGAAGCTGTAAGGGCCGACCGAATGCGTGGAGGAAGGAAATAAGTTTGGGC; CAATGTACAAGAGAGACAGGGCCCTGAAGCAACAGAAAAAAGCCCTCATCCGAGCCAATG; GACTTAAGCTAGAAGCCATGTCTCAGGTGATCCAAGCTATGNCCTCTGACCTGACATTTC; CTCTGCATTTCAAACATCCACTCTGCCTCCAAGGCCTACTTCTGAACATGCTGCCTTGCC; CTCTACAGACTATGACAGAAGTCCCTTTGGAACATCCCCCATTAGCATGACAATGCCCCC; TTCACGGAGCCTTGGAGGGTACCAAACCCATGGCCCCTTTCCTAACCCGGCCATCAAGGT; TGAGTCCCAAACCCCTTTACAACTCACCCGGATCCATAT;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_003822 unedited; CCCATTTCCGTTGNGTTTACTTGAATTTCCGGGGAAGCTACAAGTCACATAATTTACAAT; AGTAATTAAATAAACTACAGTAGGAATGATGAAGATTTACAGACATTTAATCAAGTGAAT; GATTTTAATTCATTAAGACTCTTCTATGTCCCTGAACTAAGTTCCACTAATTTGAAGACA; TGCTACAGTAAACACATATATTTGGTTTCTTCAGTCTTGGTTATATCTTATACAAAGGTG; AAACCAAGAAACAATAAATTAGCATCATAATTGTCTACAGTTGAAAGTATGATATACAAT; ATAAATACACTACTCAAAAGCCAGAAATATTTATGAAAAAATTAAGGGACTAGGAGATAC; TTTTATTTTTTACAGTTTCTACAAAGTGATTGTGCCGCCGATAATCTCAAAAAAATTAAC; GTCTAAATGAGATTCCCGTTTTTGATTTTAGTGTCTCACGACATAGAATAATTAAGTACT; GTTAAAAATAATTACCAATCAGAAAAATAACATTAAGGAAATTCCCGCTTTTGCTATTTT; GTTATATCACAGATCCCCCTGCCCCTCAGGACCTAAAGAATGTAAACACTATATCTTATT; TGACTTTAAAGTATTAATACTTTATTTTAAACAAATGTTGATCCACATCTGCACAGCTAC; CTTTTAAAATTGTGAAATAAGGCAAGACTTTCATACCTGTGGCATCGCATGTTAATAATA; CTGATAATAGTCATTTGAAGAATTGCGAGTCTTTGTCCTCCCATATTGTTCTACAGCAGA; CTTTGGTCTATGACTTAAAAGGCCAGNATCACTCTCTTCCTTTCCCCTTTGGGGAAGATT; TGAATTACTTTAATATCTTTCCAGCACCATGGTAAAATCACCATTTCCACCCCACTCTTG; G;
• Restriction Sites: NotI-NotI
• ACCN: NM_003822
• Insert Size: 4100 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_003822.3, NP_003813.1
• RefSeq Size: 4916 bp
• RefSeq ORF: 1488 bp
• Locus ID: 2494
• UniProt ID: O00482
• Cytogenetics: 1q32.1
• Domains: HOLI, zf-C4
• Protein Families: Druggable Genome, Nuclear Hormone Receptor, Transcription Factors
• Protein Pathways: Maturity onset diabetes of the young
• Summary: The protein encoded by this gene is a DNA-binding zinc finger transcription factor and is a member of the fushi tarazu factor-1 subfamily of orphan nuclear receptors. The encoded protein is involved in the expression of genes for hepatitis B virus and cholesterol biosynthesis, and may be an important regulator of embryonic development. [provided by RefSeq, Jun 2016]; Transcript Variant: This variant (2) lacks an alternate in-frame exon compared to variant 1. The resulting isoform (2) has the same N- and C-termini but is shorter compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.