NR5A2 (NM_003822) Human Untagged Clone

CAT#: SC117736

NR5A2 (untagged)-Human nuclear receptor subfamily 5, group A, member 2 (NR5A2), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: NR5A2
• Synonyms: B1F; B1F2; CPF; FTF; FTZ-F1; FTZ-F1beta; hB1F-2; LRH-1; LRH1
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_003822 edited
  ATGTCTTCTAATTCAGATACTGGGGATTTACAAGAGTCTTTAAAGCACGGACTTACACCT
  ATTGTGTCTCAATTTAAAATGGTGAATTACTCCTATGATGAAGATCTGGAAGAGCTTTGT
  CCCGTGTGTGGAGATAAAGTGTCTGGGTACCATTATGGGCTCCTCACCTGTGAAAGCTGC
  AAGGGATTTTTTAAGCGAACAGTCCAAAATAATAAAAGGTACACATGTATAGAAAACCAG
  AACTGCCAAATTGACAAAACACAGAGAAAGCGTTGTCCTTACTGTCGTTTTCAAAAATGT
  CTAAGTGTTGGAATGAAGCTAGAAGCTGTAAGGGCCGACCGAATGCGTGGAGGAAGGAAT
  AAGTTTGGGCCAATGTACAAGAGAGACAGGGCCCTGAAGCAACAGAAAAAAGCCCTCATC
  CGAGCCAATGGACTTAAGCTAGAAGCCATGTCTCAGGTGATCCAAGCTATGCCCTCTGAC
  CTGACCATTTCCTCTGCAATTCAAAACATCCACTCTGCCTCCAAAGGCCTACCTCTGAAC
  CATGCTGCCTTGCCTCCTACAGACTATGACAGAAGTCCCTTTGTAACATCCCCCATTAGC
  ATGACAATGCCCCCTCACGGCAGCCTGCAAGGTTACCAAACACATGGCCACTTTCCTAGC
  CGGGCCATCAAGTCTGAGTACCCAGACCCCTATACCAGCTCACCCGAGTCCATAATGGGC
  TATTCATATATGGATAGTTACCAGACGAGCTCTCCAGCAAGCATCCCACATCTGATACTG
  GAACTTTTGAAGTGTGAGCCAGATGAGCCTCAAGTCCAGGCTAAAATCATGGCCTATTTG
  CAGCAAGAGCAGGCTAACCGAAGCAAGCACGAAAAGCTGAGCACCTTTGGGCTTATGTGC
  AAAATGGCAGATCAAACTCTCTTCTCCATTGTCGAGTGGGCCAGGAGTAGTATCTTCTTC
  AGAGAACTTAAGGTTGATGACCAAATGAAGCTGCTTCAGAACTGCTGGAGTGAGCTCTTA
  ATCCTCGACCACATTTACCGACAAGTGGTACATGGAAAGGAAGGATCCATCTTCCTGGTT
  ACTGGGCAACAAGTGGACTATTCCATAATAGCATCACAAGCCGGAGCCACCCTCAACAAC
  CTCATGAGTCATGCACAGGAGTTAGTGGCAAAACTTCGTTCTCTCCAGTTTGATCAACGA
  GAGTTCGTATGTCTGAAATTCTTGGTGCTCTTTAGTTTAGATGTCAAAAACCTTGAAAAC
  TTCCAGCTGGTAGAAGGTGTCCAGGAACAAGTCAATGCCGCCCTGCTGGACTACACAATG
  TGTAACTACCCGCAGCAGACAGAGAAATTTGGACAGCTACTTCTTCGACTACCCGAAATC
  CGGGCCATCAGTATGCAGGCTGAAGAATACCTCTACTACAAGCACCTGAACGGGGATGTG
  CCCTATAATAACCTTCTCATTGAAATGTTGCATGCCAAAAGAGCATAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_003822 unedited
  GGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGGGNAAAACTTGCTTGTAAC
  TTTATGAATTCTGGATTTTTTTTTTTCCTTTGCTTTTTCTTAACTTTCACTAAGGGTTAC
  TGTAGTCTGATGTGTCCTTCCCAAGGCCACGAAATTTGACAAGCTGCACTTTTCTTTTGC
  TCAATGATTTCTGCTTTAAGCCAAAGAACTGCCTATAATTTCACTAAGAATGTCTTCTAA
  TTCAGATACTGGGGATTTACAAGAGTCTTTAAAGCACGGACTTACACCTATTGTGTCTCA
  ATTTAAAATGGTGAATTACTCCTATGATGAAGATCTGGAAGAGCTTTGTCCCGTGTGTGG
  AGATAAAGTGTCTGGGTACCATTATGGGCTCCTCACCTGTGAAAGCTGCAAGGGATTTTT
  TAAGCGAACAGTCCAAAATAATAAAAGGTACACATGTATAGAAAACCAGAACTGCCAAAT
  TGACAAAACACAGAGAAAGCGTTGTCCTTACTGTCGTTTTCAAAAATGTCTAAGTGTTGG
  AATGAAGCTAGAAGCTGTAAGGGCCGACCGAATGCGTGGAGGAAGGAAATAAGTTTGGGC
  CAATGTACAAGAGAGACAGGGCCCTGAAGCAACAGAAAAAAGCCCTCATCCGAGCCAATG
  GACTTAAGCTAGAAGCCATGTCTCAGGTGATCCAAGCTATGNCCTCTGACCTGACATTTC
  CTCTGCATTTCAAACATCCACTCTGCCTCCAAGGCCTACTTCTGAACATGCTGCCTTGCC
  CTCTACAGACTATGACAGAAGTCCCTTTGGAACATCCCCCATTAGCATGACAATGCCCCC
  TTCACGGAGCCTTGGAGGGTACCAAACCCATGGCCCCTTTCCTAACCCGGCCATCAAGGT
  TGAGTCCCAAACCCCTTTACAACTCACCCGGATCCATAT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_003822 unedited
  CCCATTTCCGTTGNGTTTACTTGAATTTCCGGGGAAGCTACAAGTCACATAATTTACAAT
  AGTAATTAAATAAACTACAGTAGGAATGATGAAGATTTACAGACATTTAATCAAGTGAAT
  GATTTTAATTCATTAAGACTCTTCTATGTCCCTGAACTAAGTTCCACTAATTTGAAGACA
  TGCTACAGTAAACACATATATTTGGTTTCTTCAGTCTTGGTTATATCTTATACAAAGGTG
  AAACCAAGAAACAATAAATTAGCATCATAATTGTCTACAGTTGAAAGTATGATATACAAT
  ATAAATACACTACTCAAAAGCCAGAAATATTTATGAAAAAATTAAGGGACTAGGAGATAC
  TTTTATTTTTTACAGTTTCTACAAAGTGATTGTGCCGCCGATAATCTCAAAAAAATTAAC
  GTCTAAATGAGATTCCCGTTTTTGATTTTAGTGTCTCACGACATAGAATAATTAAGTACT
  GTTAAAAATAATTACCAATCAGAAAAATAACATTAAGGAAATTCCCGCTTTTGCTATTTT
  GTTATATCACAGATCCCCCTGCCCCTCAGGACCTAAAGAATGTAAACACTATATCTTATT
  TGACTTTAAAGTATTAATACTTTATTTTAAACAAATGTTGATCCACATCTGCACAGCTAC
  CTTTTAAAATTGTGAAATAAGGCAAGACTTTCATACCTGTGGCATCGCATGTTAATAATA
  CTGATAATAGTCATTTGAAGAATTGCGAGTCTTTGTCCTCCCATATTGTTCTACAGCAGA
  CTTTGGTCTATGACTTAAAAGGCCAGNATCACTCTCTTCCTTTCCCCTTTGGGGAAGATT
  TGAATTACTTTAATATCTTTCCAGCACCATGGTAAAATCACCATTTCCACCCCACTCTTG
  G
  
• Restriction Sites: NotI-NotI
• ACCN: NM_003822
• Insert Size: 4100 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003822.3, NP_003813.1
• RefSeq Size: 4916 bp
• RefSeq ORF: 1488 bp
• Locus ID: 2494
• UniProt ID: O00482
• Cytogenetics: 1q32.1
• Domains: HOLI, zf-C4
• Protein Families: Druggable Genome, Nuclear Hormone Receptor, Transcription Factors
• Protein Pathways: Maturity onset diabetes of the young
• Gene Summary: The protein encoded by this gene is a DNA-binding zinc finger transcription factor and is a member of the fushi tarazu factor-1 subfamily of orphan nuclear receptors. The encoded protein is involved in the expression of genes for hepatitis B virus and cholesterol biosynthesis, and may be an important regulator of embryonic development. [provided by RefSeq, Jun 2016]
  Transcript Variant: This variant (2) lacks an alternate in-frame exon compared to variant 1. The resulting isoform (2) has the same N- and C-termini but is shorter compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.