WNT3A (NM_033131) Human Tagged ORF Clone
WNT3A (Myc-DDK-tagged)-Human wingless-type MMTV integration site family, member 3A (WNT3A)
|Product Name||WNT3A (NM_033131) Human Tagged ORF Clone|
|Type||Human Tagged ORF Clone|
|Synonyms||MGC119418; MGC119419; MGC119420|
|E. coli Selection||Kanamycin (25 ug/mL)|
>RC213639 representing NM_033131
Red=Cloning site Blue=ORF Green=Tags(s)
>RC213639 representing NM_033131
Red=Cloning site Green=Tags(s)
Sequencher program is needed, download here.
|Restriction Sites||SgfI-MluI Cloning Scheme for this gene Plasmid Map|
|ORF Size||1056 bp|
|OTI Disclaimer||Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at email@example.com or by calling 301.340.3188 option 3 for pricing and delivery.
The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
|OTI Annotation||This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.|
|Product Components||The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.|
|Reconstitution||1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
|RefSeq||NM_033131.1, NM_033131.2, NM_033131.3, NP_149122.1|
|RefSeq Size||2932 bp|
|RefSeq ORF||1059 bp|
|Protein Families||Adult stem cells, Cancer stem cells, Druggable Genome, ES Cell Differentiation/IPS, Induced pluripotent stem cells, Secreted Protein, Stem cell relevant signaling - Wnt Signaling pathway|
|Protein Pathways||Basal cell carcinoma, Hedgehog signaling pathway, Melanogenesis, Pathways in cancer, Wnt signaling pathway|
|Gene Summary||The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It encodes a protein which shows 96% amino acid identity to mouse Wnt3A protein, and 84% to human WNT3 protein, another WNT gene product. This gene is clustered with WNT14 gene, another family member, in chromosome 1q42 region. [provided by RefSeq, Jul 2008]|
|The use of this cDNA Clones has been cited in the following citations:|
Non-Canonical Wnt Predominates in Activated Rat Hepatic Stellate Cells, Influencing HSC Survival and Paracrine Stimulation of Kupffer Cells
,Corbett, L;Mann, J;Mann, DA;,
PLoS ONE Nov 2015
,PubMed ID 26566235
|cDNA Clone Resources|
|SC305570||WNT3A (untagged)-Human wingless-type MMTV integration site family, member 3A (WNT3A)||
|RG213639||WNT3A (GFP-tagged) - Human wingless-type MMTV integration site family, member 3A (WNT3A)||
|RC213639L1||Lenti ORF clone of Human wingless-type MMTV integration site family, member 3A (WNT3A), Myc-DDK-tagged||
|RC213639L2||Lenti ORF clone of Human wingless-type MMTV integration site family, member 3A (WNT3A), mGFP tagged||
|RC213639L3||Lenti ORF clone of Human wingless-type MMTV integration site family, member 3A (WNT3A), Myc-DDK-tagged||
|RC213639L4||Lenti ORF clone of Human wingless-type MMTV integration site family, member 3A (WNT3A), mGFP tagged||