PLA2R (PLA2R1) (NM_001007267) Human Tagged ORF Clone

CAT#: RC213576

PLA2R1 (Myc-DDK-tagged)-Human phospholipase A2 receptor 1, 180kDa (PLA2R1), transcript variant 2

Product Images

Circular map for RC213576

• 

  Western blot validation of overexpression lysate (Cat# LY423477) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC213576 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified PLA2R1 protein (Cat# TP313576). The protein was produced from HEK293T cells transfected with PLA2R1 cDNA clone (Cat# RC213576) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PLA2R
• Synonyms: CLEC13C; PLA2-R; PLA2G1R; PLA2IR; PLA2R
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC213576 representing NM_001007267
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGCTGTCGCCGTCGCTGCTGCTGCTGCTGCTGCTGGGGGCGCCGCGGGGCTGCGCCGAGGGTGTGG
  CGGCGGCGCTTACCCCCGAGCGGCTCCTGGAGTGGCAGGATAAAGGAATATTTGTTATCCAAAGTGAGAG
  TCTCAAGAAATGCATTCAAGCAGGTAAATCGGTTCTGACCCTGGAGAACTGCAAGCAAGCAAACAAGCAC
  ATGCTGTGGAAATGGGTTTCAAACCATGGCCTCTTTAACATAGGAGGCAGTGGTTGCCTGGGCCTGAATT
  TCTCCGCCCCAGAGCAGCCATTAAGCTTATATGAATGTGACTCCACCCTCGTTTCCTTACGGTGGCGCTG
  TAACAGGAAGATGATCACAGGCCCGCTGCAGTACTCTGTCCAGGTGGCGCATGACAACACAGTGGTGGCC
  TCACGGAAGTATATTCATAAGTGGATTTCTTATGGGTCAGGTGGTGGAGACATTTGTGAATATCTACACA
  AAGATTTGCATACAATCAAAGGGAACACCCACGGGATGCCGTGTATGTTTCCCTTCCAGTATAACCATCA
  GTGGCATCATGAATGTACCCGTGAAGGTCGGGAAGATGACTTACTGTGGTGTGCCACGACAAGCCGTTAT
  GAAAGAGATGAAAAGTGGGGATTTTGCCCTGATCCCACCTCTGCAGAAGTAGGTTGTGATACTATTTGGG
  AGAAGGACCTCAATTCACACATTTGCTACCAGTTCAACCTGCTTTCATCTCTCTCTTGGAGTGAGGCACA
  TTCTTCATGCCAGATGCAAGGAGGTACGCTGTTAAGTATTACAGATGAAACTGAAGAAAATTTCATAAGG
  GAGCACATGAGCAGTAAAACAGTGGAGGTGTGGATGGGCCTCAATCAGCTGGATGAACACGCTGGCTGGC
  AGTGGTCTGATGGAACGCCGCTCAACTATCTGAATTGGAGCCCAGAGGTAAATTTTGAGCCATTTGTTGA
  AGATCACTGTGGAACATTTAGTTCATTTATGCCAAGTGCCTGGAGGAGTCGGGATTGTGAGTCCACCTTG
  CCATATATATGTAAAAAATATCTAAACCACATTGATCATGAAATAGTTGAAAAAGATGCGTGGAAATATT
  ATGCTACCCACTGTGAGCCTGGCTGGAATCCCTACAATCGTAATTGCTACAAACTTCAGAAAGAAGAAAA
  GACCTGGCATGAGGCTCTGCGTTCTTGTCAGGCTGATAACAGTGCATTAATAGACATAACCTCATTAGCA
  GAGGTGGAGTTTCTTGTAACCCTCCTTGGAGATGAAAATGCATCAGAAACATGGATTGGTTTGAGCAGCA
  ATAAAATTCCAGTTTCCTTTGAATGGTCTAATGACTCTTCAGTCATCTTTACTAATTGGCACACACTTGA
  GCCCCACATTTTTCCAAATAGAAGCCAGCTGTGTGTCTCAGCAGAGCAGTCTGAGGGACACTGGAAAGTC
  AAAAATTGTGAAGAAAGACTTTTTTACATTTGTAAAAAAGCAGGCCATGTCCTCTCTGATGCTGAATCAG
  GATGTCAAGAGGGATGGGAGAGACATGGTGGATTCTGTTACAAAATTGACACAGTCCTTCGAAGCTTTGA
  CCAAGCTTCCAGCGGTTATTACTGTCCTCCTGCACTTGTAACCATTACAAACAGGTTTGAACAGGCTTTT
  ATTACCAGTTTGATCAGTAGTGTGGTAAAAATGAAGGACAGTTATTTTTGGATAGCTCTTCAGGACCAAA
  ATGATACGGGAGAATACACTTGGAAGCCAGTAGGGCAGAAACCCGAGCCGGTGCAGTACACACACTGGAA
  CACACACCAGCCGCGCTACAGTGGTGGCTGTGTTGCCATGCGAGGAAGGCATCCACTTGGTCGCTGGGAA
  GTGAAGCACTGTCGGCACTTTAAGGCAATGTCCTTGTGCAAGCAGCCAGTTGAAAATCAGGAAAAAGCAG
  AGTATGAAGAGAGATGGCCCTTTCACCCCTGCTATTTGGACTGGGAGTCAGAGCCTGGTCTGGCCAGTTG
  CTTCAAGGTATTTCATAGTGAAAAAGTTCTGATGAAAAGAACATGGAGAGAAGCTGAAGCATTTTGCGAA
  GAATTTGGAGCTCATCTTGCAAGCTTTGCCCATATTGAGGAAGAGAATTTTGTGAATGAGCTCTTACATT
  CAAAATTTAATTGGACAGAAGAAAGGCAGTTCTGGATTGGATTTAATAAAAGAAACCCACTGAATGCCGG
  CTCATGGGAGTGGTCTGATAGAACTCCTGTTGTCTCTTCGTTTTTAGACAACACTTATTTTGGAGAAGAT
  GCAAGAAACTGTGCTGTTTATAAGGCAAACAAAACATTGCTGCCCTTACACTGTGGTTCCAAACGTGAAT
  GGATATGCAAAATCCCAAGAGATGTGAAACCCAAGATTCCGTTCTGGTACCAGTACGATGTACCCTGGCT
  CTTTTATCAGGATGCAGAATACCTTTTTCATACCTTTGCCTCAGAATGGTTGAACTTTGAGTTTGTCTGT
  AGCTGGCTGCACAGTGATCTTCTCACAATTCATTCTGCACATGAGCAAGAATTCATCCACAGCAAAATAA
  AAGCGCTATCAAAGTATGGTGCAAGTTGGTGGATTGGACTTCAAGAAGAAAGAGCCAATGATGAATTTCG
  CTGGAGAGATGGAACACCAGTGATATACCAGAACTGGGACACAGGAAGAGAAAGAACTGTGAATAATCAG
  AGCCAGAGATGTGGCTTTATTTCTTCTATAACAGGACTCTGGGGTAGTGAAGAGTGTTCAGTTTCTATGC
  CTAGTATCTGTAAGCGAAAAAAGGTTTGGCTCATAGAGAAAAAGAAAGATACACCAAAACAACATGGAAC
  GTGTCCCAAAGGATGGCTATATTTTAACTATAAGTGCCTTCTGCTGAATATCCCCAAAGACCCAAGCAGT
  TGGAAGAACTGGACGCATGCTCAACATTTCTGTGCTGAAGAAGGGGGGACCCTGGTCGCCATTGAAAGTG
  AGGTGGAGCAAGCTTTCATTACTATGAATCTTTTTGGCCAGACCACCAGTGTGTGGATAGGTTTACAAAA
  TGATGATTATGAAACATGGCTAAATGGAAAGCCTGTGGTATATTCTAACTGGTCTCCATTTGATATAATA
  AATATTCCAAGTCACAATACCACTGAAGTTCAGAAACACATTCCTCTCTGTGCCTTACTCTCAAGTAATC
  CTAATTTTCATTTCACTGGAAAATGGTATTTTGAAGACTGTGGAAAGGAAGGCTATGGGTTTGTTTGTGA
  AAAAATGCAAGATACTTCTGGACACGGTGTAAATACATCTGATATGTATCCAATGCCCAATACCTTAGAA
  TATGGAAACAGAACTTACAAAATAATTAATGCAAATATGACTTGGTATGCAGCAATAAAAACCTGCCTGA
  TGCACAAAGCACAACTGGTCAGCATCACAGACCAGTATCACCAGTCCTTCCTCACTGTTGTCCTCAACCG
  GCTAGGATATGCCCACTGGATTGGACTGTTCACCACAGATAATGGTCTTAATTTTGACTGGTCTGATGGC
  ACCAAATCTTCTTTCACTTTTTGGAAAGATGAGGAGTCCTCCCTCCTTGGTGACTGCGTTTTTGCCGACA
  GCAACGGACGCTGGCATAGCACAGCCTGCGAGTCATTTCTGCAAGGTGCCATTTGTCATGTGCCACCTGA
  AACAAGACAATCTGAACACCCAGAGTTGTGCTCAGAAACATCTATTCCCTGGATAAAATTTAAAAGTAAT
  TGCTACAGTTTTTCTACAGTCCTAGACAGTATGAGTTTTGAGGCTGCTCATGAATTTTGCAAAAAGGAAG
  GTTCTAATCTTTTAACAATCAAGGATGAGGCTGAAAATGCATTTCTCCTAGAAGAGCTGTTTGCTTTTGG
  TTCTTCTGTCCAGATGGTTTGGTTGAATGCTCAATTTGATGGTAACAGTAAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC213576 representing NM_001007267
  Red=Cloning site Green=Tags(s)
  
  MLLSPSLLLLLLLGAPRGCAEGVAAALTPERLLEWQDKGIFVIQSESLKKCIQAGKSVLTLENCKQANKH
  MLWKWVSNHGLFNIGGSGCLGLNFSAPEQPLSLYECDSTLVSLRWRCNRKMITGPLQYSVQVAHDNTVVA
  SRKYIHKWISYGSGGGDICEYLHKDLHTIKGNTHGMPCMFPFQYNHQWHHECTREGREDDLLWCATTSRY
  ERDEKWGFCPDPTSAEVGCDTIWEKDLNSHICYQFNLLSSLSWSEAHSSCQMQGGTLLSITDETEENFIR
  EHMSSKTVEVWMGLNQLDEHAGWQWSDGTPLNYLNWSPEVNFEPFVEDHCGTFSSFMPSAWRSRDCESTL
  PYICKKYLNHIDHEIVEKDAWKYYATHCEPGWNPYNRNCYKLQKEEKTWHEALRSCQADNSALIDITSLA
  EVEFLVTLLGDENASETWIGLSSNKIPVSFEWSNDSSVIFTNWHTLEPHIFPNRSQLCVSAEQSEGHWKV
  KNCEERLFYICKKAGHVLSDAESGCQEGWERHGGFCYKIDTVLRSFDQASSGYYCPPALVTITNRFEQAF
  ITSLISSVVKMKDSYFWIALQDQNDTGEYTWKPVGQKPEPVQYTHWNTHQPRYSGGCVAMRGRHPLGRWE
  VKHCRHFKAMSLCKQPVENQEKAEYEERWPFHPCYLDWESEPGLASCFKVFHSEKVLMKRTWREAEAFCE
  EFGAHLASFAHIEEENFVNELLHSKFNWTEERQFWIGFNKRNPLNAGSWEWSDRTPVVSSFLDNTYFGED
  ARNCAVYKANKTLLPLHCGSKREWICKIPRDVKPKIPFWYQYDVPWLFYQDAEYLFHTFASEWLNFEFVC
  SWLHSDLLTIHSAHEQEFIHSKIKALSKYGASWWIGLQEERANDEFRWRDGTPVIYQNWDTGRERTVNNQ
  SQRCGFISSITGLWGSEECSVSMPSICKRKKVWLIEKKKDTPKQHGTCPKGWLYFNYKCLLLNIPKDPSS
  WKNWTHAQHFCAEEGGTLVAIESEVEQAFITMNLFGQTTSVWIGLQNDDYETWLNGKPVVYSNWSPFDII
  NIPSHNTTEVQKHIPLCALLSSNPNFHFTGKWYFEDCGKEGYGFVCEKMQDTSGHGVNTSDMYPMPNTLE
  YGNRTYKIINANMTWYAAIKTCLMHKAQLVSITDQYHQSFLTVVLNRLGYAHWIGLFTTDNGLNFDWSDG
  TKSSFTFWKDEESSLLGDCVFADSNGRWHSTACESFLQGAICHVPPETRQSEHPELCSETSIPWIKFKSN
  CYSFSTVLDSMSFEAAHEFCKKEGSNLLTIKDEAENAFLLEELFAFGSSVQMVWLNAQFDGNSK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001007267
• ORF Size: 3972 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001007267.2, NP_001007268.1
• RefSeq Size: 5160 bp
• RefSeq ORF: 3975 bp
• Locus ID: 22925
• UniProt ID: Q13018
• Cytogenetics: 2q24.2
• Protein Families: Druggable Genome, Transmembrane
• MW: 152.81 kDa
• Gene Summary: This gene represents a phospholipase A2 receptor. The encoded protein likely exists as both a transmembrane form and a soluble form. The transmembrane receptor may play a role in clearance of phospholipase A2, thereby inhibiting its action. Polymorphisms at this locus have been associated with susceptibility to idiopathic membranous nephropathy. Alternatively spliced transcript variants encoding different isoforms have been identified.[provided by RefSeq, Sep 2010]