PLA2R (PLA2R1) (NM_001007267) Human Untagged Clone

CAT#: SC301201

PLA2R1 (untagged)-Human phospholipase A2 receptor 1, 180kDa (PLA2R1), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PLA2R
• Synonyms: CLEC13C; PLA2-R; PLA2G1R; PLA2IR; PLA2R
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC301201 representing NM_001007267.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGCTGCTGTCGCCGTCGCTGCTGCTGCTGCTGCTGCTGGGGGCGCCGCGGGGCTGCGCCGAGGGTGTG
  GCGGCGGCGCTTACCCCCGAGCGGCTCCTGGAGTGGCAGGATAAAGGAATATTTGTTATCCAAAGTGAG
  AGTCTCAAGAAATGCATTCAAGCAGGTAAATCGGTTCTGACCCTGGAGAACTGCAAGCAAGCAAACAAG
  CACATGCTGTGGAAATGGGTTTCAAACCATGGCCTCTTTAACATAGGAGGCAGTGGTTGCCTGGGCCTG
  AATTTCTCCGCCCCAGAGCAGCCATTAAGCTTATATGAATGTGACTCCACCCTCGTTTCCTTACGGTGG
  CGCTGTAACAGGAAGATGATCACAGGCCCGCTGCAGTACTCTGTCCAGGTGGCGCATGACAACACAGTG
  GTGGCCTCACGGAAGTATATTCATAAGTGGATTTCTTATGGGTCAGGTGGTGGAGACATTTGTGAATAT
  CTACACAAAGATTTGCATACAATCAAAGGGAACACCCACGGGATGCCGTGTATGTTTCCCTTCCAGTAT
  AACCATCAGTGGCATCATGAATGTACCCGTGAAGGTCGGGAAGATGACTTACTGTGGTGTGCCACGACA
  AGCCGTTATGAAAGAGATGAAAAGTGGGGATTTTGCCCTGATCCCACCTCTGCAGAAGTAGGTTGTGAT
  ACTATTTGGGAGAAGGACCTCAATTCACACATTTGCTACCAGTTCAACCTGCTTTCATCTCTCTCTTGG
  AGTGAGGCACATTCTTCATGCCAGATGCAAGGAGGTACGCTGTTAAGTATTACAGATGAAACTGAAGAA
  AATTTCATAAGGGAGCACATGAGCAGTAAAACAGTGGAGGTGTGGATGGGCCTCAATCAGCTGGATGAA
  CACGCTGGCTGGCAGTGGTCTGATGGAACGCCGCTCAACTATCTGAATTGGAGCCCAGAGGTAAATTTT
  GAGCCATTTGTTGAAGATCACTGTGGAACATTTAGTTCATTTATGCCAAGTGCCTGGAGGAGTCGGGAT
  TGTGAGTCCACCTTGCCATATATATGTAAAAAATATCTAAACCACATTGATCATGAAATAGTTGAAAAA
  GATGCGTGGAAATATTATGCTACCCACTGTGAGCCTGGCTGGAATCCCTACAATCGTAATTGCTACAAA
  CTTCAGAAAGAAGAAAAGACCTGGCATGAGGCTCTGCGTTCTTGTCAGGCTGATAACAGTGCATTAATA
  GACATAACCTCATTAGCAGAGGTGGAGTTTCTTGTAACCCTCCTTGGAGATGAAAATGCATCAGAAACA
  TGGATTGGTTTGAGCAGCAATAAAATTCCAGTTTCCTTTGAATGGTCTAATGACTCTTCAGTCATCTTT
  ACTAATTGGCACACACTTGAGCCCCACATTTTTCCAAATAGAAGCCAGCTGTGTGTCTCAGCAGAGCAG
  TCTGAGGGACACTGGAAAGTCAAAAATTGTGAAGAAAGACTTTTTTACATTTGTAAAAAAGCAGGCCAT
  GTCCTCTCTGATGCTGAATCAGGATGTCAAGAGGGATGGGAGAGACATGGTGGATTCTGTTACAAAATT
  GACACAGTCCTTCGAAGCTTTGACCAAGCTTCCAGCGGTTATTACTGTCCTCCTGCACTTGTAACCATT
  ACAAACAGGTTTGAACAGGCTTTTATTACCAGTTTGATCAGTAGTGTGGTAAAAATGAAGGACAGTTAT
  TTTTGGATAGCTCTTCAGGACCAAAATGATACGGGAGAATACACTTGGAAGCCAGTAGGGCAGAAACCC
  GAGCCGGTGCAGTACACACACTGGAACACACACCAGCCGCGCTACAGTGGTGGCTGTGTTGCCATGCGA
  GGAAGGCATCCACTTGGTCGCTGGGAAGTGAAGCACTGTCGGCACTTTAAGGCAATGTCCTTGTGCAAG
  CAGCCAGTTGAAAATCAGGAAAAAGCAGAGTATGAAGAGAGATGGCCCTTTCACCCCTGCTATTTGGAC
  TGGGAGTCAGAGCCTGGTCTGGCCAGTTGCTTCAAGGTATTTCATAGTGAAAAAGTTCTGATGAAAAGA
  ACATGGAGAGAAGCTGAAGCATTTTGCGAAGAATTTGGAGCTCATCTTGCAAGCTTTGCCCATATTGAG
  GAAGAGAATTTTGTGAATGAGCTCTTACATTCAAAATTTAATTGGACAGAAGAAAGGCAGTTCTGGATT
  GGATTTAATAAAAGAAACCCACTGAATGCCGGCTCATGGGAGTGGTCTGATAGAACTCCTGTTGTCTCT
  TCGTTTTTAGACAACACTTATTTTGGAGAAGATGCAAGAAACTGTGCTGTTTATAAGGCAAACAAAACA
  TTGCTGCCCTTACACTGTGGTTCCAAACGTGAATGGATATGCAAAATCCCAAGAGATGTGAAACCCAAG
  ATTCCGTTCTGGTACCAGTACGATGTACCCTGGCTCTTTTATCAGGATGCAGAATACCTTTTTCATACC
  TTTGCCTCAGAATGGTTGAACTTTGAGTTTGTCTGTAGCTGGCTGCACAGTGATCTTCTCACAATTCAT
  TCTGCACATGAGCAAGAATTCATCCACAGCAAAATAAAAGCGCTATCAAAGTATGGTGCAAGTTGGTGG
  ATTGGACTTCAAGAAGAAAGAGCCAATGATGAATTTCGCTGGAGAGATGGAACACCAGTGATATACCAG
  AACTGGGACACAGGAAGAGAAAGAACTGTGAATAATCAGAGCCAGAGATGTGGCTTTATTTCTTCTATA
  ACAGGACTCTGGGGTAGTGAAGAGTGTTCAGTTTCTATGCCTAGTATCTGTAAGCGAAAAAAGGTTTGG
  CTCATAGAGAAAAAGAAAGATACACCAAAACAACATGGAACGTGTCCCAAAGGATGGCTATATTTTAAC
  TATAAGTGCCTTCTGCTGAATATCCCCAAAGACCCAAGCAGTTGGAAGAACTGGACGCATGCTCAACAT
  TTCTGTGCTGAAGAAGGGGGGACCCTGGTCGCCATTGAAAGTGAGGTGGAGCAAGCTTTCATTACTATG
  AATCTTTTTGGCCAGACCACCAGTGTGTGGATAGGTTTACAAAATGATGATTATGAAACATGGCTAAAT
  GGAAAGCCTGTGGTATATTCTAACTGGTCTCCATTTGATATAATAAATATTCCAAGTCACAATACCACT
  GAAGTTCAGAAACACATTCCTCTCTGTGCCTTACTCTCAAGTAATCCTAATTTTCATTTCACTGGAAAA
  TGGTATTTTGAAGACTGTGGAAAGGAAGGCTATGGGTTTGTTTGTGAAAAAATGCAAGATACTTCTGGA
  CACGGTGTAAATACATCTGATATGTATCCAATGCCCAATACCTTAGAATATGGAAACAGAACTTACAAA
  ATAATTAATGCAAATATGACTTGGTATGCAGCAATAAAAACCTGCCTGATGCACAAAGCACAACTGGTC
  AGCATCACAGACCAGTATCACCAGTCCTTCCTCACTGTTGTCCTCAACCGGCTAGGATATGCCCACTGG
  ATTGGACTGTTCACCACAGATAATGGTCTTAATTTTGACTGGTCTGATGGCACCAAATCTTCTTTCACT
  TTTTGGAAAGATGAGGAGTCCTCCCTCCTTGGTGACTGCGTTTTTGCCGACAGCAACGGACGCTGGCAT
  AGCACAGCCTGCGAGTCATTTCTGCAAGGTGCCATTTGTCATGTGCCACCTGAAACAAGACAATCTGAA
  CACCCAGAGTTGTGCTCAGAAACATCTATTCCCTGGATAAAATTTAAAAGTAATTGCTACAGTTTTTCT
  ACAGTCCTAGACAGTATGAGTTTTGAGGCTGCTCATGAATTTTGCAAAAAGGAAGGTTCTAATCTTTTA
  ACAATCAAGGATGAGGCTGAAAATGCATTTCTCCTAGAAGAGCTGTTTGCTTTTGGTTCTTCTGTCCAG
  ATGGTTTGGTTGAATGCTCAATTTGATGGTAACAGTAAGTGA
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001007267
• Insert Size: 3975 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001007267.2
• RefSeq Size: 5175 bp
• RefSeq ORF: 3975 bp
• Locus ID: 22925
• UniProt ID: Q13018
• Cytogenetics: 2q24.2
• Protein Families: Druggable Genome, Transmembrane
• MW: 152.8 kDa
• Gene Summary: This gene represents a phospholipase A2 receptor. The encoded protein likely exists as both a transmembrane form and a soluble form. The transmembrane receptor may play a role in clearance of phospholipase A2, thereby inhibiting its action. Polymorphisms at this locus have been associated with susceptibility to idiopathic membranous nephropathy. Alternatively spliced transcript variants encoding different isoforms have been identified.[provided by RefSeq, Sep 2010]
  Transcript Variant: This variant (2) uses an alternate splice site in the 3' coding region, compared to variant 1, resulting in a shorter protein. It encodes a secreted isoform (2), which lacks a C-terminal transmembrane region found in isoform 1.