BMP1 (NM_001199) Human Tagged ORF Clone

CAT#: RC212538

BMP1 (Myc-DDK-tagged)-Human bone morphogenetic protein 1 (BMP1), transcript variant 1

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY BMP1 (Cat# RC212538, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BMP1(Cat# TA505813). Positive lysates LY400480 (100ug) and LC400480 (20ug) can be purchased separately from OriGene.

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  Western blot validation of overexpression lysate (Cat# LY400480) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC212538 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: BMP1
• Synonyms: OI13; PCOLC; PCP; PCP2; TLD
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC212538 representing NM_001199
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCCGGCGTGGCCCGCCTGCCGCTGCTGCTCGGGCTGCTGCTGCTCCCGCGTCCCGGCCGGCCGCTGG
  ACTTGGCCGACTACACCTATGACCTGGCGGAGGAGGACGACTCGGAGCCCCTCAACTACAAAGACCCCTG
  CAAGGCGGCTGCCTTTCTTGGGGACATTGCCCTGGACGAAGAGGACCTGAGGGCCTTCCAGGTACAGCAG
  GCTGTGGATCTCAGACGGCACACAGCTCGTAAGTCCTCCATCAAAGCTGCAGTTCCAGGAAACACTTCTA
  CCCCCAGCTGCCAGAGCACCAACGGGCAGCCTCAGAGGGGAGCCTGTGGGAGATGGAGAGGTAGATCCCG
  TAGCCGGCGGGCGGCGACGTCCCGACCAGAGCGTGTGTGGCCCGATGGGGTCATCCCCTTTGTCATTGGG
  GGAAACTTCACTGGTAGCCAGAGGGCAGTCTTCCGGCAGGCCATGAGGCACTGGGAGAAGCACACCTGTG
  TCACCTTCCTGGAGCGCACTGACGAGGACAGCTATATTGTGTTCACCTATCGACCTTGCGGGTGCTGCTC
  CTACGTGGGTCGCCGCGGCGGGGGCCCCCAGGCCATCTCCATCGGCAAGAACTGTGACAAGTTCGGCATT
  GTGGTCCACGAGCTGGGCCACGTCGTCGGCTTCTGGCACGAACACACTCGGCCAGACCGGGACCGCCACG
  TTTCCATCGTTCGTGAGAACATCCAGCCAGGGCAGGAGTATAACTTCCTGAAGATGGAGCCTCAGGAGGT
  GGAGTCCCTGGGGGAGACCTATGACTTCGACAGCATCATGCATTACGCTCGGAACACATTCTCCAGGGGC
  ATCTTCCTGGATACCATTGTCCCCAAGTATGAGGTGAACGGGGTGAAACCTCCCATTGGCCAAAGGACAC
  GGCTCAGCAAGGGGGACATTGCCCAAGCCCGCAAGCTTTACAAGTGCCCAGCCTGTGGAGAGACCCTGCA
  AGACAGCACAGGCAACTTCTCCTCCCCTGAATACCCCAATGGCTACTCTGCTCACATGCACTGCGTGTGG
  CGCATCTCTGTCACACCCGGGGAGAAGATCATCCTGAACTTCACGTCCCTGGACCTGTACCGCAGCCGCC
  TGTGCTGGTACGACTATGTGGAGGTCCGAGATGGCTTCTGGAGGAAGGCGCCCCTCCGAGGCCGCTTCTG
  CGGGTCCAAACTCCCTGAGCCTATCGTCTCCACTGACAGCCGCCTCTGGGTTGAATTCCGCAGCAGCAGC
  AATTGGGTTGGAAAGGGCTTCTTTGCAGTCTACGAAGCCATCTGCGGGGGTGATGTGAAAAAGGACTATG
  GCCACATTCAATCGCCCAACTACCCAGACGATTACCGGCCCAGCAAAGTCTGCATCTGGCGGATCCAGGT
  GTCTGAGGGCTTCCACGTGGGCCTCACATTCCAGTCCTTTGAGATTGAGCGCCACGACAGCTGTGCCTAC
  GACTATCTGGAGGTGCGCGACGGGCACAGTGAGAGCAGCACCCTCATCGGGCGCTACTGTGGCTATGAGA
  AGCCTGATGACATCAAGAGCACGTCCAGCCGCCTCTGGCTCAAGTTCGTCTCTGACGGGTCCATTAACAA
  AGCGGGCTTTGCCGTCAACTTTTTCAAAGAGGTGGACGAGTGCTCTCGGCCCAACCGCGGGGGCTGTGAG
  CAGCGGTGCCTCAACACCCTGGGCAGCTACAAGTGCAGCTGTGACCCCGGGTACGAGCTGGCCCCAGACA
  AGCGCCGCTGTGAGGCTGCCTGTGGCGGATTCCTCACCAAGCTCAACGGCTCCATCACCAGCCCGGGCTG
  GCCCAAGGAGTACCCCCCCAACAAGAACTGCATCTGGCAGCTGGTGGCCCCCACCCAGTACCGCATCTCC
  CTGCAGTTTGACTTCTTTGAGACAGAGGGCAATGATGTGTGCAAGTACGACTTCGTGGAGGTGCGCAGTG
  GACTCACAGCTGACTCCAAGCTGCATGGCAAGTTCTGTGGTTCTGAGAAGCCCGAGGTCATCACCTCCCA
  GTACAACAACATGCGCGTGGAGTTCAAGTCCGACAACACCGTGTCCAAAAAGGGCTTCAAGGCCCACTTC
  TTCTCAGAAAAGAGGCCAGCTCTGCAGCCCCCTCGGGGACGCCCCCACCAGCTCAAATTCCGAGTGCAGA
  AAAGAAACCGGACCCCCCAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC212538 representing NM_001199
  Red=Cloning site Green=Tags(s)
  
  MPGVARLPLLLGLLLLPRPGRPLDLADYTYDLAEEDDSEPLNYKDPCKAAAFLGDIALDEEDLRAFQVQQ
  AVDLRRHTARKSSIKAAVPGNTSTPSCQSTNGQPQRGACGRWRGRSRSRRAATSRPERVWPDGVIPFVIG
  GNFTGSQRAVFRQAMRHWEKHTCVTFLERTDEDSYIVFTYRPCGCCSYVGRRGGGPQAISIGKNCDKFGI
  VVHELGHVVGFWHEHTRPDRDRHVSIVRENIQPGQEYNFLKMEPQEVESLGETYDFDSIMHYARNTFSRG
  IFLDTIVPKYEVNGVKPPIGQRTRLSKGDIAQARKLYKCPACGETLQDSTGNFSSPEYPNGYSAHMHCVW
  RISVTPGEKIILNFTSLDLYRSRLCWYDYVEVRDGFWRKAPLRGRFCGSKLPEPIVSTDSRLWVEFRSSS
  NWVGKGFFAVYEAICGGDVKKDYGHIQSPNYPDDYRPSKVCIWRIQVSEGFHVGLTFQSFEIERHDSCAY
  DYLEVRDGHSESSTLIGRYCGYEKPDDIKSTSSRLWLKFVSDGSINKAGFAVNFFKEVDECSRPNRGGCE
  QRCLNTLGSYKCSCDPGYELAPDKRRCEAACGGFLTKLNGSITSPGWPKEYPPNKNCIWQLVAPTQYRIS
  LQFDFFETEGNDVCKYDFVEVRSGLTADSKLHGKFCGSEKPEVITSQYNNMRVEFKSDNTVSKKGFKAHF
  FSEKRPALQPPRGRPHQLKFRVQKRNRTPQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001199
• ORF Size: 2190 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001199.4
• RefSeq Size: 2487 bp
• RefSeq ORF: 2193 bp
• Locus ID: 649
• UniProt ID: P13497
• Cytogenetics: 8p21.3
• Domains: CUB, Astacin, EGF_CA, ZnMc, EGF, EGF
• Protein Families: Druggable Genome, Protease
• MW: 82.9 kDa
• Gene Summary: This gene encodes a protein that is capable of inducing formation of cartilage in vivo. Although other bone morphogenetic proteins are members of the TGF-beta superfamily, this gene encodes a protein that is not closely related to other known growth factors. This gene is expressed as alternatively spliced variants that share an N-terminal protease domain but differ in their C-terminal region. [provided by RefSeq, Aug 2008]